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91.
Predation on Protozoa: its importance to zooplankton 总被引:34,自引:0,他引:34
Protozoa are an important component of both the nano- and microplanktonin marine and freshwater environments and are preyed upon byzooplankton, including suspension-feeding cope pods, some gelatinouszoopiankters and some first-feeding fish larvae. The clearancerates of suspension-feeding zooplankton for ciliates, in particular,are higher than for most phytoplankton. For at least some suspension-feedingzooplankton, protozoans are calculated to be quantitativelyan important component of the diet during certain seasons. Inlaboratory studies, protozoan components in the diet appearto enhance growth and survival of certain life-history stagesor enhance fecundity. These data suggest that protozoans arequalitatively as well as quantitatively important in the dietsof marine zooplankton. Most studies of predation on Protozoahave focused on the euphotic zone in nearshore waters. Predationon Protozoa is expected, however, to be particularly importantboth quantitatively and qualitatively in marine environmentsand seasons in which primary production is dominated by cells<5 µm in size, such as nearshore environments afterthe spring phytoplankton bloom, in oligotrophic waters, andin environments dominated by detritus-dominated food webs, suchas the deep sea. In detritus-dominated food webs, Protozoa maybe a source of essential nutrients and may thus facilitate utilizationof bacterial and detrital carbon by metazoan plankton. 相似文献
92.
Diane R. Campbell 《Evolution; international journal of organic evolution》1996,50(4):1442-1453
Genetic variances, heritabilities, and genetic correlations of floral traits were measured in the monocarpic perennial Ipomopsis aggregata (Polemoniaceae). A paternal half-sib design was employed to generate seeds in each of four years, and seeds were planted back in the field near the parental site. The progeny were followed for up to eight years to estimate quantitative genetic parameters subject to natural levels of environmental variation over the entire life cycle. Narrow-sense heritabilities of 0.2–0.8 were detected for the morphometric traits of corolla length, corolla width, stigma position, and anther position. The proportion of time spent by the protandrous flowers in the pistillate phase (“proportion pistillate”) also exhibited detectable heritability of near 0.3. In contrast, heritability estimates for nectar reward traits were low and not significantly different from zero, due to high environmental variance between and within flowering years. The estimates of genetic parameters were combined with phenotypic selection gradients to predict evolutionary responses to selection mediated by the hummingbird pollinators. One trait, corolla width, showed the potential for a rapid response to ongoing selection through male function, as it experienced both direct selection, by influencing pollen export, and relatively high heritability. Predicted responses were lower for proportion pistillate and corolla length, even though these traits also experienced direct selection. Stigma position was expected to respond positively to indirect selection of proportion pistillate but negatively to selection of corolla length, with the net effect sensitive to variation in the selection estimates. Anther position also was not directly selected but could respond to indirect selection of genetically correlated traits. 相似文献
93.
Dissolved organic carbon (DOC) in Lake Fryxell, 10 streams flowing into the lake, and the moat surrounding the lake was studied to determine the influence of sources and biogeochemical processes on its distribution and chemical nature. Lake Fryxell is an amictic, permanently ice-covered lake in the McMurdo Dry Valleys which contains benthic and planktonic microbial populations, but receives essentially no input of organic material from the ahumic soils of the watershed. Biological activity in the water column does not appear to influence the DOC depth profile, which is similar to the profiles for conservative inorganic constituents. DOC values for the streams varied with biomass in the stream channel, and ranged from 0.2 to 9.7 mg C/L. Fulvic acids in the streams were a lower percentage of the total DOC than in the lake. These samples contain recent carbon and appear to be simpler mixtures of compounds than the lake samples, indicating that they have undergone less humification. The fulvic acids from just above the sediments of the lake have a high sulfur content and are highly aliphatic. The main transformations occurring as these fractions diffuse upward in the water column are 1) loss of sulfur groups through the oxycline and 2) decrease in aliphatic carbon and increase in the heterogeneity of aliphatic moieties. The fraction of modem14C content of the lake fulvic acids range from a minimum of 0.68 (approximately 3000 years old) at 15m depth to 0.997 (recent material) just under the ice. The major processes controlling the DOC in the lake appear to be: 1) The transport of organic matter by the inflow streams resulting in the addition of recent organic material to the moat and upper waters of the lake; 2) The diffusion of organic matter composed of relict organic material and organic carbon resulting from the degradation of algae and bacteria from the bottom waters or sediments of the lake into overlying glacial melt water, 3) The addition of recent organic matter to the bottom waters of the lake from the moat. 相似文献
94.
95.
Bioluminescent Salmonella typhimurium provides a rapid assay for measuring the efficacy of freeze-drying suspension media 总被引:1,自引:1,他引:0
A strain of Salmonella typhimurium , transformed to a bioluminescent phenotype, was used to compare three freeze-drying suspension media: inositol serum broth with and without added gelatin and sterile skimmed milk. Recovery and growth studies performed by measuring changes in bioluminescence demonstrated that of the three media tested, the routinely used inositol serum broth was the most effective freeze-drying suspension medium. 相似文献
96.
Simon Lovestone C. Hugh Reynolds Donna Latimer Daniel R. Davis Brian H. Anderton Jean-Marc Gallo Diane Hanger Sandrine Mulot Betina Marquardt Silvia Stabel James R. Woodgett Christopher C. J. Miller 《Current biology : CB》1994,4(12)
Background: Paired helical filaments (PHFs) are a characteristic pathological feature of Alzheimer's disease; their principal component is the microtubule-associated protein tau. The tau in PHFs (PHF-tau) is hyperphosphorylated, but the cellular mechanisms responsible for this hyperphosphorylation have yet to be elucidated. A number of kinases, including mitogen-activated protein (MAP) kinase, glycogen synthase kinase (GSK)-3α, GSK-3β and cyclin-dependent kinase-5, phosphorylate recombinant tau in vitro so that it resembles PHF-tau as judged by its reactivity with a panel of antibodies capable of discriminating between normal tau and PHF-tau, and by a reduced electrophoretic mobility that is characteristic of PHF-tau. To determine whether MAP kinase, GSK-3α and GSK-3β can also induce Alzheimer's disease-like phosphorylation of tau in mammalian cells, we studied the phosphorylation status of tau in primary neuronal cultures and transfected COS cells following changes in the activities of MAP kinase and GSK-3.Results Activating MAP kinase in cultures of primary neurons or transfected COS cells expressing tau isoforms did not increase the level of phosphorylation for any PHF-tau epitope investigated. But elevating GSK-3 activity in the COS cells by co-transfection with GSK-3α or GSK-3β decreased the electrophoretic mobility of tau so that it resembled that of PHF-tau, and induced reactivity with eight PHF-tau-selective monoclonal antibodies.Conclusion Our data indicate that GSK-3α and/or GSK-3β, but not MAP kinase, are good candidates for generating PHF-type phosphorylation of tau in Alzheimer's disease. The involvement of other kinases in the generation of PHFs cannot, however, be eliminated. Our results suggest that aberrant regulation of GSK-3 may be a pathogenic mechanism in Alzheimer's disease. 相似文献
97.
Frédérique Tihy Nicolas Vogt Dominique Recan Bernard Malfoy France Leturcq Michelle Coquet Françoise Serville Daniel Fontan Jean-Michel Guillard Jean-Claude Kaplan Bernard Dutrillaux Nicole Lemieux 《Human genetics》1994,93(5):563-567
A girl with severe Becker muscular dystrophy and apparently normal chromosomes had a heterozygous deletion for exons 51, 52, and 53 of the dystrophin gene. This deletion was transmitted by her mother, who was unaffected. To differentiate the normal and the deleted X chromosomes, fluorescence in situ hybridization (FISH) was applied to metaphase chromosomes, using probes for both exons 51 and 52, which are only 388 and 113 base pairs long, respectively. FISH signals were observed in one or both chromatids of one chromosome, but never on both chromosomes, suggesting the lack of hybridization on the deleted X chromosome. Using 5-bromodeoxyuridine incorporation to differentiate the late (inactive) and the early replicating (active) X chromosomes, 77% of the signals were observed on the active X chromosomes in the mother. This percentage was only 18% in the daughter, suggesting that skewed inactivation of the X chromosomes was responsible for the phenotypic differences. 相似文献
98.
Diane Wagner 《Oecologia》1993,96(2):276-281
The transfer of nutrients between organisms is a common feature of mutualism. The production of these food rewards is often assumed to be costly. Estimation of the costs of producing food rewards is important for understanding the overall effects of the interaction on fitness. When food rewards are harvested by several species differing in foraging behavior, costs to the producer may differ. The larvae of many species in the butterfly family Lycaenidae produce secretions consumed by tending ants. Here I report that three North American ant species, Formica perpilosa, Dorymyrmex sp. (smithi complex), and Forelius foetida, had no negative effect on the duration of development and adult size of the lycaenid Hemiargus isola. Moreover, tending by the ant Formica perpilosa significantly enhanced larval growth, resulting in butterflies that were 20% heavier than their untended counterparts. Tending by the ants Dorymyrmex sp. (smithi complex) and Forelius foetida had no effect on butterfly weight. Tended, nonfeeding larvae lost 69% more weight than untended, nonfeeding larvae. Taken together, the results suggest that, although ant tending imposes a physiological cost, H. isola larvae use behavioral or physiological mechanisms to compensate or overcompensate for nutrients lost to ants. 相似文献
99.
Summary Ubiquinone-10 (Q10) production was measured in batch cultures of Paracoccus denitrificans grown for 8 h at increasing oxygen concentrations (0–21 % O2 in the sparging gas). Whereas the cellular level of Q10 decreased monotonically from 1.2 to 0.5 mol/g d.w., the total yield of Q10 was maximal at 2.5 % O2 and amounted to 350 nmol (0.3 mg) per L of culture. 相似文献
100.
Diane E. Eyer 《Human nature (Hawthorne, N.Y.)》1994,5(1):69-94
A study of the research on postpartum mother-infant bonding shows that results from poorly constructed research programs were
published in major journals and became a part of hospital policy because the bonding concept was politically useful in the
struggle between advocates of natural childbirth and managers of the medical model of birth. The concept was also uncritically
accepted because it was consistent with a longstanding ideology of motherhood that sees women as the prime architects of their
children’s personalities.
Diane Eyer earned her Ph.D. in developmental psychology from the University of Pennsylvania. She is currently writing a book
on the ways in which the concepts of bonding and attachment have affected our understanding of appropriate early childcare. 相似文献