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41.
42.
A soluble carotenoid protein involved in phycobilisome-related energy dissipation in cyanobacteria 总被引:9,自引:0,他引:9 下载免费PDF全文
Photosynthetic organisms have developed multiple protective mechanisms to survive under high-light conditions. In plants, one of these mechanisms is the thermal dissipation of excitation energy in the membrane-bound chlorophyll antenna of photosystem II. The question of whether or not cyanobacteria, the progenitor of the chloroplast, have an equivalent photoprotective mechanism has long been unanswered. Recently, however, evidence was presented for the possible existence of a mechanism dissipating excess absorbed energy in the phycobilisome, the extramembrane antenna of cyanobacteria. Here, we demonstrate that this photoprotective mechanism, characterized by blue light-induced fluorescence quenching, is indeed phycobilisome-related and that a soluble carotenoid binding protein, ORANGE CAROTENOID PROTEIN (OCP), encoded by the slr1963 gene in Synechocystis PCC 6803, plays an essential role in this process. Blue light is unable to quench fluorescence in the absence of phycobilisomes or OCP. The fluorescence quenching is not DeltapH-dependent, and it can be induced in the absence of the reaction center II or the chlorophyll antenna, CP43 and CP47. Our data suggest that OCP, which strongly interacts with the thylakoids, acts as both the photoreceptor and the mediator of the reduction of the amount of energy transferred from the phycobilisomes to the photosystems. These are novel roles for a soluble carotenoid protein. 相似文献
43.
Tidal salt marshes in the San Francisco Estuary region display heterogeneous vegetation patterns that influence wetland function and provide adequate habitat for native or endangered wildlife. In addition to analyzing the extent of vegetation, monitoring the dynamics of vegetation pattern within restoring wetlands can offer valuable information about the restoration process. Pattern metrics, derived from classified remotely sensed imagery, have been used to measure composition and configuration of patches and landscapes, but they can be unpredictable across scales, and inconsistent across time. We sought to identify pattern metrics that are consistent across spatial scale and time – and thus robust measures of vegetation and habitat configuration – for a restored tidal marsh in the San Francisco Bay, CA, USA. We used high-resolution (20 cm) remotely sensed color infrared imagery to map vegetation pattern over 2 years, and performed a multi-scale analysis of derived vegetation pattern metrics. We looked at the influence on metrics of changes in grain size through resampling and changes in minimum mapping unit (MMU) through smoothing. We examined composition, complexity, connectivity and heterogeneity metrics, focusing on perennial pickleweed (Sarcocornia pacifica), a dominant marsh plant. At our site, pickleweed patches grew larger, more irregularly shaped, and closely spaced over time, while the overall landscape became more diverse. Of the two scale factors examined, grain size was more consistent than MMU in terms of identifying relative change in composition and configuration of wetland marsh vegetation over time. Most metrics exhibited unstable behavior with larger MMUs. With small MMUs, most metrics were consistent across grain sizes, from fine (e.g. 0.16 m2) to relatively large (e.g. 16 m2) pixel sizes. Scale relationships were more variable at the landcover class level than at the landscape level (across all classes). This information may be useful to applied restoration practitioners, and adds to our general understanding of vegetation change in a restoring marsh. 相似文献
44.
Background
Uterine leiomyomas, or fibroids, represent the most common benign tumor of the female reproductive tract. Fibroids become symptomatic in 30% of all women and up to 70% of African American women of reproductive age. Epigenetic dysregulation of individual genes has been demonstrated in leiomyoma cells; however, the in vivo genome-wide distribution of such epigenetic abnormalities remains unknown.Principal Findings
We characterized and compared genome-wide DNA methylation and mRNA expression profiles in uterine leiomyoma and matched adjacent normal myometrial tissues from 18 African American women. We found 55 genes with differential promoter methylation and concominant differences in mRNA expression in uterine leiomyoma versus normal myometrium. Eighty percent of the identified genes showed an inverse relationship between DNA methylation status and mRNA expression in uterine leiomyoma tissues, and the majority of genes (62%) displayed hypermethylation associated with gene silencing. We selected three genes, the known tumor suppressors KLF11, DLEC1, and KRT19 and verified promoter hypermethylation, mRNA repression and protein expression using bisulfite sequencing, real-time PCR and western blot. Incubation of primary leiomyoma smooth muscle cells with a DNA methyltransferase inhibitor restored KLF11, DLEC1 and KRT19 mRNA levels.Conclusions
These results suggest a possible functional role of promoter DNA methylation-mediated gene silencing in the pathogenesis of uterine leiomyoma in African American women. 相似文献45.
Characterization of a model cyanobacterium Synechocystis sp. PCC 6803 autotrophic growth in a flat‐panel photobioreactor 下载免费PDF全文
Tomáš Zavřel Maria A. Sinetova Diana Búzová Petra Literáková Jan Červený 《Engineering in Life Science》2015,15(1):122-132
We characterized the photoautotrophic growth of glucose‐tolerant Synechocystis sp. PCC 6803 in a flat‐panel photobioreactor running on a semicontinuous regime under various lights, temperatures, and influx carbon dioxide concentrations. The maximum reached growth rate was 0.135 h?1, which corresponds to a doubling time of 5.13 h—a growth speed never reported for Synechocystis before. Saturating red light intensity for the strain was 220–360 μmol(photons) m?2 s?1, and we did not observe any photoinhibition up to 660 μmol(photons) m?2 s?1. Synechocystis was able to grow under red light only; however, photons of wavelengths 405–585 and 670–700 nm further improved its growth. Optimal growth temperature was 35°C. Below 32°C, the growth rates decreased linearly with temperature coefficient (Q10) 1.70. Semicontinuous cultivation is known to be efficient for growth characterization and optimization. However, the assumption of correct growth rates calculation—culture exponential growth—is often not fulfilled. The semicontinuous setup in this study was operated as a turbidostat. Accurate online OD measurements with high time‐resolution allowed fast and reliable growth rates determination. Repeating diluting frequencies (up to 18 dilutions per day) were essential for rapid growth stability evaluation. The presented setup provides improvement to previously published semicontinuous characterization strategies by decreasing experimental time requirements and maintaining the culture in exponential growth phase throughout the entire characterization procedure. 相似文献
46.
High-level production of 1,3-propanediol from crude glycerol by <Emphasis Type="Italic">Clostridium butyricum</Emphasis> AKR102a 总被引:1,自引:0,他引:1
Wilkens E Ringel AK Hortig D Willke T Vorlop KD 《Applied microbiology and biotechnology》2012,93(3):1057-1063
The aim of this study was to optimize a biotechnological process for the production of 1,3-propanediol (1,3-PD) based on low-quality
crude glycerol derived from biodiesel production. Clostridium butyricum AKR102a was used in fed-batch fermentations in 1-L and 200-L scale. The newly discovered strain is characterized by rapid
growth, high product tolerance, and the ability to use crude glycerol at the lowest purity directly gained from a biodiesel
plant side stream. Using pure glycerol, the strain AKR102 reached 93.7 g/L 1,3-PD with an overall productivity of 3.3 g/(L*h).
With crude glycerol under the same conditions, 76.2 g/L 1,3-PD was produced with a productivity of 2.3 g/(L*h). These are
among the best results published so far for natural producers. The scale up to 200 L was possible. Due to the simpler process
design, only 61.5 g/L 1,3-PD could be reached with a productivity of 2.1 g/(L*h). 相似文献
47.
48.
D. Matteuzzi Patrizia Brigidi Maddalena Rossi Diana Di 《Letters in applied microbiology》1990,11(4):220-223
The small cryptic plasmid pMB1 (1.9 kb), previously isolated from Bifidobacterium longum, has been characterized by physical mapping. Two cloning vectors, pMR3 and pDG7, carrying chloramphenicol and ampicillin resistances derived from pJH101, have been electroporated in Escherichia coli. 相似文献
49.
Diana Paola Gómez-Mendoza Ana Carolina Lara-Ribeiro Thiago Verano-Braga 《Biochimica et Biophysica Acta - Proteins and Proteomics》2021,1869(6):140622
Cardiac remodeling involves cellular and structural changes that occur as consequence of multifactorial events to maintain the homeostasis. The progression of pathological cardiac remodeling involves a transition from adaptive to maladaptive changes that eventually leads to impairment of ventricular function and heart failure. In this scenario, proteins are key elements that orchestrate molecular events as increased expression of fetal genes, neurohormonal and second messengers' activation, contractile dysfunction, rearrangement of the extracellular matrix and alterations in heart geometry. Mass spectrometry based-proteomics has emerged as a sound method to study protein dysregulation and identification of cardiac diseases biomarkers in plasma. In this review, we summarize the main findings related to large-scale proteome modulation of cardiac cells and extracellular matrix occurred during pathological cardiac remodeling. We describe the recent proteomic progresses in the selection of protein targets and introduce the renin-angiotensin system as an interesting target for the treatment of pathological cardiac remodeling. 相似文献
50.
Rogier M. Thurlings Carla A. Wijbrandts Roelof J. Bennink Serge E. Dohmen Carlijn Voermans Diana Wouters Elena S. Izmailova Danielle M. Gerlag Berthe L. F. van Eck-Smit Paul P. Tak 《PloS one》2009,4(11)