皱纹盘鲍外套膜、鳃和足粘液细胞的类型与分布

以阿新兰和过碘酸雪夫氏反应(AB．PAS)染色法显微观察皱纹盘鲍(1taliotis discus hannai)的外套膜、鳃和足的粘液细胞。根据所显示颜色的不同,可将粘液细胞分为Ⅰ～Ⅳ4种类型：分别呈红色、蓝色、紫红色和蓝紫色。外套触手和外套膜上皮的粘液细胞以Ⅱ型为主,Ⅳ型较少,密度不均,多为近圆形细胞,大型和小型细胞均有分布。鳃轴和鳃叶上皮的粘液细胞密度较大,以Ⅱ型和Ⅰ型为主,Ⅲ型和Ⅳ型较少,形态有杯形、近圆形或棒状等,多为中型及小型细胞。足的上皮粘液细胞较少,均为Ⅱ型,但局部上皮的细胞含有许多棕色颗粒。     

有丝分裂细胞死亡

细胞死亡有坏死、凋亡、裂亡、自体吞噬等多种方式。细胞裂亡指细胞经过一次有丝分裂后才开始死亡的现象。本文综述了对于细胞裂亡这种新型细胞死亡方式的初步认识。     

Chromatography studies on bio-affinity of nine ligands of a1-adrenoceptor to a1D subtypes overexpressed in cell membrane

Copyright by Science in China Press 2004 The a1-AR is present in many tissues including heart, blood vessels, liver, brain, kidney, prostate and spleen. In these tissues, the a1-AR mediates a variety of physiological effects such as neurotransmission, vasoconstriction, cardiacinotropy, chronotropy and glycogenolysis[1]. In 1986, Morrow and Greese pro-posed that a1-AR should be subclassified into two subtypes. Later this hypothesis was proved by Q.D Han in 1987. He not only verified a1-A…     

我国蝗虫暴发成灾的现状及其持续控制对策

概述并分析了我国蝗虫暴发成灾的现状及原因 ,认为全球气候变暖和区域性气候异常、人类活动的影响、监测预警技术手段落后、成灾蝗种增多和发生为害类型复杂、蝗虫研究队伍锐减及基础理论与高新技术研究滞后导致了近年来蝗虫的暴发成灾。对此 ,作者从蝗虫灾害的规律性、监测预警高新技术和综合治理技术等方面提出了实现蝗灾可持续控制的对策     

马尾松诱导抗性对马尾松毛虫幼虫生长发育的影响

利用不同受害方式和受害程度的马尾松 (PinusmassonianaLamb.)针饲养马尾松毛DendrolimuspunctarusWalker虫幼虫 ,研究受害松针对幼虫生长发育的影响。结果表明 :马尾松毛虫取食受害松针后 ,幼虫的取食量下降 ,排粪量减少 ,对食物的消耗和利用指数降低 ,幼虫发育速率缓慢 ,死亡率增加 ,从而影响马尾松毛虫的种群动态     

胸腺素α原融合蛋白基因表达、纯化与生物学活性

胸腺素α原及胸腺九肽在体内免疫方面有重要作用 .根据大肠杆菌遗传密码的偏爱性 ,设计并人工合成了胸腺素α原及胸腺九肽融合蛋白的特异引物 .以胸腺素α原cDNA为模板 ,应用PCR及分子克隆技术构建了该融合蛋白的编码基因 ,将其插入pBV2 2 0质粒 ,转化大肠杆菌 ,通过温度诱导使该融合蛋白高效表达 ,并对表达产物进行了高效特异性纯化 .运用MTT法初步测定了该融合蛋白的活性 .结果表明 ,该融合蛋白对氢化可的松诱导的胸腺细胞损伤有一定的保护作用 ,为今后进一步研究和应用该融合蛋白奠定了基础     

Dynamics of Aboveground and Soil Carbon and Nitrogen Stocks and Cycling of Available Nitrogen along a Land-use Gradient in Rondônia,Brazil

High rates of deforestation in the Brazilian Amazon have the potential to alter the storage and cycling of carbon (C) and nitrogen (N) across this region. To investigate the impacts of deforestation, we quantified total aboveground biomass (TAGB), aboveground and soil pools of C and N, and soil N availability along a land-use gradient in Rondônia, Brazil, that included standing primary forest, slashed primary and secondary forest, shifting cultivation, and pasture sites. TAGB decreased substantially with increasing land use, ranging from 311 and 399 Mg ha^–1 (primary forests) to 63 Mg ha^–1 (pasture). Aboveground C and N pools declined in patterns and magnitudes similar to those of TAGB. Unlike aboveground pools, soil C and N concentrations and pools did not show consistent declines in response to land use. Instead, C and N concentrations were strongly related to percent clay content of soils. Concentrations of NO₃-N and NH₄-N generally increased in soils following slash-and-burn events along the land-use gradient and decreased with increasing land use. Increasing land use resulted in marked declines in NO₃-N pools relative to NH₄-N pools. Rates of net nitrification and N-mineralization were also generally higher in postfire treatments relative to prefire treatments along the land-use gradient and declined with increasing land use. Results demonstrate the linked responses of aboveground C and N pools and soil N availability to land use in the Brazilian Amazon; steady reductions in aboveground pools along the land-use gradient were accompanied by declines in inorganic soil N pools and transformation rates.     

An outbreak of dengue virus at Rio de Janeiro--1986

Dengue virus type 1 has been isolated in Aedes albopictus cell strain, from sera of patients living in the Nova Igua?u county, by Rio de Janeiro. The clinical picture was characterized by fever, headache, retrobulbar pain, backache, pains in the muscles and the joints and prostration. Studies in paired sera confirmed the presence of recent infection by dengue virus type 1. The outbreak reached adjacent areas, including Rio de Janeiro city (May, 1986).     

LncRNA OIP5-AS1 regulates radioresistance by targeting DYRK1A through miR-369-3p in colorectal cancer cells

Object

This study aimed to investigate the role of lncRNA OIP5-AS1 in regulating radioresistance of colorectal cancer (CRC) cells.