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161.
Ligand binding to a linear lattice composed of N sites, under general conditions of cooperativity and number of sites covered upon binding, m, is approached in terms of the theory of contracted partition functions. The partition function of the system obeys a recursion relation leading to a generating function that provides an exact analytical solution for any case of interest. Site-specific properties of the lattice are derived from simple transformations of the analytical expressions. The McGhee-von Hippel model is obtained as a special case in the limit N --> infinity. The derivation is straightforward and involves no combinatorial arguments. Partition functions and site-specific properties are also derived for the case of non-cooperative binding to a two-dimensional torus of length N, containing s sites in its section for a total of sN sites. The torus provides a relevant model for ligand binding to double-stranded DNA (s = 2) or protein helices (s = 3,4). It is proved that non-cooperative binding to the two-dimensional torus can mimic cooperative binding to a one-dimensional linear lattice when m = s. The dimensional embedding of the lattice and the geometry of interaction of its sites play a crucial role in defining the binding properties of the system accessible to experimental measurements. Hence, caution must be exercised in the interpretation of Scatchard plots in terms of the one-dimensional McGhee-von Hippel model, especially when m < or = 4 and the geometry of the system is clearly two-dimensional. 相似文献
162.
The distribution of Keratella species from 15 different lakes in North Patagonia (Argentina) was analysed. The genus was not present at altitudes above 1000 m. K. tropica was restricted to Patagonian Plateau lakes with a comparatively high conductivity. A morphometric analysis of the widely distributed K. cochlearis was performed. Results showed three groups of K. cochlearis corresponding to Andean lakes, Patagonian Plateau lakes and a Patagonian Reservoir. 相似文献
163.
We present a list of Italian freshwater and marine Oligochaeta in the families Lumbriculidae, Haplotaxidae, Tubificidae, Naididae, Propappidae, Criodrilidae, and Lumbricidae, representing 57 genera and 130 species. Published data reflect the incomplete knowledge of the Italian oligochaete fauna, restricted to certain geographical areas. Subterranean aquatic and marine fauna are of particular interest as these have been studied the least. We provide a comprehensive review of the Naididae including, for the first time, southern Italy and the islands of Sicily and Sardinia. The distribution of species is discussed and taxonomic problems arising from the morphological variability of Italian material are examined. 相似文献
164.
Emilio Fernandez 《Plant Molecular Biology Reporter》1996,14(4):295-295
165.
166.
Prof. Renato Meduri Emilio Campos Lucia Scorolli Caterina De Vinci Giancarlo Pizza Dimitri Viza 《Biotherapy》1996,9(1-3):61-66
Recurrent ocular herpes is an insoluble problem for the clinician. As cellular immunity plays an important role in controlling
herpes relapses, and other studies have shown the efficacy of HSV-specific transfer factor (TF) for the treatment of herpes
patients, an open clinical trial was undertaken in 134 patients (71 keratitis, 29 kerato-uveitis, 34 uveitis) suffering from
recurrent ocular herpetic infections. The mean duration of the treatment was 358 days, and the entire follow-up period 189121
before, and 64062 days after TF treatment. The cell-mediated immune response to the viral antigens, evaluated by the lymphocyte
stimulation test (LST) and the leucocyte migration test (LMT) (P<0.001), was significantly increased by the TF treatment.
The total number of relapses was decreased significantly during/after TF treatment, dropping from 832 before, to 89 after
treatment, whereas the cumulative relapse index (RI) dropped, during the same period, from 13.2 to 4.17 (P<0.0001). No side
effects were observed. It is concluded that patients with relapsing ocular herpes can benefit from treatment with HSV-specific
TF. 相似文献
167.
A组轮状病毒SA11VP6基因的克隆和表达 总被引:4,自引:0,他引:4
从SA11VP6基因全序列克隆开始,设计一对两端带有酶切位点的引物,逆转录PCR扩增出VP6全基因CDNA。经酶切后插入PUC19,构建了VP6全基因克隆PRA6。再经酶切后插入痘苗病毒载休质凿PJSA1175中。利用Lipofectin导入TK143细胞,利用TK基因和Lac基因作为重组病毒的筛选标记。表达产物用单克隆抗体ELISA法检测,发现细胞培养上清和细胞裂解液都是阳性。Western b 相似文献
168.
G. Bestetti P. Di Gennaro E. Galli B. Leoni F. Pelizzoni G. Sello D. Bianchi 《Applied microbiology and biotechnology》1994,40(6):791-793
The Pseudomonas fluorescens N3 was isolated from soil for its ability to utilize naphthalene as a carbon source. The strain transforms 2,3-dimethyl-, 2-methoxy-, 1- and 2-ethylnaphthalenes to the corresponding salicylic acids competitively with chemical synthesis. The identification of 2-hydroxy-2-carboxy-7-ethylchromane by biotransformation of 2-ethylnaphthalene, contributes to elucidating the steps involved in the catabolic pathways of naphthalenes to salicylaldehydes.
Correspondence to: F. Pelizzoni 相似文献
169.
Compartmentalization of SHC, GRB2 and mSOS, and hyperphosphorylation of Raf-1 by EGF but not insulin in liver parenchyma. 总被引:15,自引:1,他引:14 下载免费PDF全文
Rat liver parenchyma harbors equal numbers of epidermal growth factor (EGF) and insulin receptors. Following administration of a saturating dose of EGF (10 micrograms/100 g body weight), there was a rapid (t1/2 approximately 1.1 min) internalization of receptor coincident with its tyrosine phosphorylation at residue 1173 and receptor recruitment of the adaptor protein SHC, its tyrosine phosphorylation and its association with GRB2 and the Ras guanine nucleotide exchange factor, mSOS, largely in endosomes. This led to a cytosolic pool of a complex of tyrosine-phosphorylated SHC, GRB2 and mSOS. It was demonstrated that these constituents were linked to Ras activation by the characteristic decrease in Raf-1 mobility on SDS-PAGE, which was maintained for 60 min after a single bolus of administered EGF. While insulin administration (15 micrograms/100 g body weight) led to insulin receptor beta-subunit tyrosine phosphorylation and internalization, there was little detectable tyrosine phosphorylation of SHC, recruitment of GRB2, association of a complex with mSOS or any detectable change in the mobility of Raf-1. Therefore, in normal physiological target cells in vivo, distinct signaling pathways are realized after EGF or insulin receptor activation, with regulation of this specificity most probably occurring at the locus of the endosome. 相似文献
170.