排序方式: 共有39条查询结果,搜索用时 109 毫秒
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Bart Van Puyvelde Sander Willems Ralf Gabriels Simon Daled Laura De Clerck Sofie Vande Casteele An Staes Francis Impens Dieter Deforce Lennart Martens Sven Degroeve Maarten Dhaenens 《Proteomics》2020,20(3-4)
Data‐independent acquisition (DIA) generates comprehensive yet complex mass spectrometric data, which imposes the use of data‐dependent acquisition (DDA) libraries for deep peptide‐centric detection. Here, it is shown that DIA can be redeemed from this dependency by combining predicted fragment intensities and retention times with narrow window DIA. This eliminates variation in library building and omits stochastic sampling, finally making the DIA workflow fully deterministic. Especially for clinical proteomics, this has the potential to facilitate inter‐laboratory comparison. 相似文献
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Briand E Salmain M Herry JM Perrot H Compère C Pradier CM 《Biosensors & bioelectronics》2006,22(3):440-448
Immunosensors, based on the immobilization of a model rabbit antibody on mixed self-assembled monolayers and Protein A as a linking agent on gold transducers, were elaborated and characterized at each step by modulated polarization-infrared spectroscopy (PM-IRRAS) and occasionally by atomic force microscopy (AFM) and quartz crystal microbalance (QCM). By testing two different mixed SAMs comprising 11-mercaptoundecanoic acid (MUA), together with either decanethiol (C9CH3) or mercaptohexanol (C6OH), the role of the chemical composition and structure of the antibody attachment layer upon the sensor performance was demonstrated. 相似文献
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Dana Ghanem Hélène Tran Claire-Marie Dhaenens Suzanna Schraen-Maschke Bernard Sablonnière Luc Buée Nicolas Sergeant Marie-Laure Caillet-Boudin 《FEBS letters》2009,583(4):675-679
Among the different mechanisms underlying the etiopathogenesis of myotonic dystrophy type 1 (DM1), a backward reprogramming to a foetal splicing machinery is an interesting hypothesis. To address this possibility, Tau splicing, which is regulated during development and modified in DM1, was analyzed. Indeed, a preferential expression of the foetal Tau isoform, instead of the six normally found, is observed in adult DM1 brains. By using two cell lines, we show here that the cis-regulating elements necessary to generate the unique foetal Tau isoform are dispensable to reproduce the trans-dominant effect induced by DM1 mutation on Tau exon 2 inclusion. Our results suggest that the mis-splicing of Tau in DM1 is resulting from a disease-associated mechanism. 相似文献
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Elisabeth Govaert Katleen Van Steendam Ellen Scheerlinck Liesbeth Vossaert Paulien Meert Martina Stella Sander Willems Laura De Clerck Maarten Dhaenens Dieter Deforce 《Proteomics》2016,16(23):2937-2944
Extracting histones from cells is the first step in studies that aim to characterize histones and their post‐translational modifications (hPTMs) with MS. In the last decade, label‐free quantification is more frequently being used for MS‐based histone characterization. However, many histone extraction protocols were not specifically designed for label‐free MS. While label‐free quantification has its advantages, it is also very susceptible to technical variation. Here, we adjust an established histone extraction protocol according to general label‐free MS guidelines with a specific focus on minimizing sample handling. These protocols are first evaluated using SDS‐PAGE. Hereafter, a selection of extraction protocols was used in a complete histone workflow for label‐free MS. All protocols display nearly identical relative quantification of hPTMs. We thus show that, depending on the cell type under investigation and at the cost of some additional contaminating proteins, minimizing sample handling can be done during histone isolation. This allows analyzing bigger sample batches, leads to reduced technical variation and minimizes the chance of in vitro alterations to the hPTM snapshot. Overall, these results allow researchers to determine the best protocol depending on the resources and goal of their specific study. Data are available via ProteomeXchange with identifier PXD002885. 相似文献
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Citrullination is a posttranslational modification of arginine. It plays both a physiological role, for instance during apoptosis and epigenetics, and a pathological role in cancer or diseases of the central nervous system. Most research on citrullination to date focuses on its role in auto-immune diseases such as multiple sclerosis and rheumatoid arthritis. In this context, the exact knowledge of citrullination sites in a protein can provide invaluable information about the etiological importance of these citrullinated proteins. However, few techniques exist that can accurately detect citrullination on the peptide level. This review aims to give an overview of the different methods available to date for the detection of citrullinated proteins and peptides. These include 2D-SDS-PAGE and immunodetection, as well as specific mass spectrometry (MS) approaches, both labeled and unlabeled. These MS approaches have been developed to pinpoint the exact location of citrullination on the peptide level. Improving the currently existing detection strategies while focusing on the role of citrullinated proteins will be invaluable to elucidate the importance of this posttranslational modification in vivo. 相似文献
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Guido van Mierlo René A.M. Dirks Laura De Clerck Arie B. Brinkman Michelle Huth Susan L. Kloet Nehmé Saksouk Leonie I. Kroeze Sander Willems Matthias Farlik Christoph Bock Joop H. Jansen Dieter Deforce Michiel Vermeulen Jérôme Déjardin Maarten Dhaenens Hendrik Marks 《Cell Stem Cell》2019,24(1):123-137.e8
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IJk van Hattum Marta Costas-Rodríguez Kasper Hobin Frank Vanhaecke Hans Vandendriessche Hélène Collet Pierre Cattelain Michel Toussaint Quentin Goffette Maarten Dhaenens Jessica L. A. Palmer Simon Daled Philippe Crombé Isabelle De Groote 《American journal of physical anthropology》2023,181(2):231-249
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