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11.
B R Deshpande V L Kallapur K Venkatesh 《Archives internationales de physiologie et de biochimie》1984,92(2):65-72
The quantitative determination of succinic dehydrogenase (SDH), hexokinase (HK), phosphorylase, phosphofructokinase (PFK), glycerol-3-phosphate dehydrogenase (G-3-PDH) and lactate dehydrogenase (LDH) was carried out in the homologous leg muscles of two aquatic Birds. It appears that the leg muscle fibres of the coot, a surface swimmer are more oxidative in nature and appear to utilize glucose as source of energy. The leg muscles of the dabchick, a diving Bird, on the other hand, seem to depend on glycogen as source of energy. The relative activity levels of HK, phosphorylase and PFK support the accepted r?le of glycogen as primary substrate of carbohydrate catabolism in the leg muscles. The ratio of G-3-PDH/LDH in the leg muscles revealed that glycerol 3-phosphate cycle appears to be insufficient to account for the major part of NADH oxidation. However, the LDH activity is quite high in all the muscles. These results led us to believe that glycerol 3-phosphate cycle may function during rest, when the rate of glycolysis will be low. 相似文献
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V. K. Sudhakaran B. S. Deshpande J. G. Shewale S. R. Naik 《Biotechnology letters》1992,14(10):913-918
Summary A recirculated packed bed batch reactor has been designed for the production of 6-aminopenicillanic acid. It was observed that the flow rate of penicillin G solution is a rate limiting step for its hydrolysis. Under the conditions used, the maximum rate of hydrolysis of penicillin G was observed at a flow rate of 3.0 L/min. 相似文献
13.
Separation and identification of prostaglandins in canine bile was performed by extraction and thin layer chromatography. The system provided tentative identification of the prostaglandin F compounds as the major prostaglandin subgroup present in bile. The prostaglandin was subsequently purified on silicic acid columns and quantitated by radioimmunoassay with tritiated PGF2α and anti PGF2α antibody employing the double antibody technique. Basal levels in hepatic bile were found to be 1028 ± 98 pg/ml. 相似文献
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Culture conditions are described for the production of extracellular β-d-xylosidase (xylobiase, exo-1,4-β-d-xylosidase, 1,4-β-d-xylan xylohydrolase, EC 3.2.1.37) in shake flasks by Sclerotium rolfsii. At the 1% cellulose level, a maximum activity of 0.82 U ml?1is obtained in media containing either 1% corn steep liquor or 1% defatted coconut cake. The β-d-xylosidase has a molecular weight of 170 000 and catalyses the hydrolysis of 4-nitrophenyl-β-d-xylopyranoside optimally at pH 4.5 and 50°C. The energy of activation is 44 kJ mol?1and the pI and Kmare 6.8 and 0.038 mm, respectively. 相似文献
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Naidu S. Chowdari Chin Pan Chetana Rao David R. Langley Prasanna Sivaprakasam Bilal Sufi Daniel Derwin Yichong Wang Eilene Kwok David Passmore Vangipuram S. Rangan Shrikant Deshpande Pina Cardarelli Gregory Vite Sanjeev Gangwar 《Bioorganic & medicinal chemistry letters》2019,29(3):466-470
Uncialamycin analogs were evaluated as potential cytotoxic agents in an antibody-drug conjugate (ADC) approach to treating human cancer. These analogs were synthesized using Hauser annulations of substituted phthalides as a key step. A highly potent uncialamycin analog 3c with a valine-citrulline dipeptide linker was conjugated to an anti-mesothelin monoclonal antibody (mAb) through lysines to generate a meso-13 conjugate. This conjugate demonstrated subnanomolar potency (IC50?=?0.88?nM, H226 cell line) in in vitro cytotoxicity experiments with good immunological specificity to mesothelin-positive lung cancer cell lines. The potency and mechanism of action of this uncialamycin class of enediyne antitumor antibiotics make them attractive payloads in ADC-based cancer therapy. 相似文献
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Biological nitrification inhibition (BNI) activity in sorghum and its characterization 总被引:5,自引:0,他引:5
G. V. Subbarao K. Nakahara T. Ishikawa H. Ono M. Yoshida T. Yoshihashi Yiyong Zhu H. A. K. M. Zakir S. P. Deshpande C. T. Hash K. L. Sahrawat 《Plant and Soil》2013,366(1-2):243-259