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121.
122.
Deshmukh R Latchumanadhas K Mullasari AS Pandurangi UM 《Indian pacing and electrophysiology journal》2008,8(3):211-217
We report two cases of patients of cardiac resynchronization therapy (CRT) whose ECGs, during follow up, showed different paced QRS morphology as compared to those of immediate post-device implantation. Parameters of leads, including sensitivity and capture thresholds, were unchanged. There was no lead dislodgement confirmed on fluoroscopy. The ECGs obtained in device off mode showed different intrinsic QRS morphology as compared to those of pre-implant morphology. These changes were attributable to electrolyte imbalance in one patient and progressive intraventricular conduction defect in the other. These cases demonstrate that intrinsic myocardial conduction pattern influences paced QRS morphology. Irreversible change in paced QRS morphology may indicate poor prognosis. 相似文献
123.
Rebecca S LaRue Stefán R Jónsson Kevin AT Silverstein Mathieu Lajoie Denis Bertrand Nadia El-Mabrouk Isidro Hötzel Valgerdur Andrésdóttir Timothy PL Smith Reuben S Harris 《BMC molecular biology》2008,9(1):104
Background
APOBEC3 (A3) proteins deaminate DNA cytosines and block the replication of retroviruses and retrotransposons. Each A3 gene encodes a protein with one or two conserved zinc-coordinating motifs (Z1, Z2 or Z3). The presence of one A3 gene in mice (Z2–Z3) and seven in humans, A3A-H (Z1a, Z2a-Z1b, Z2b, Z2c-Z2d, Z2e-Z2f, Z2g-Z1c, Z3), suggests extraordinary evolutionary flexibility. To gain insights into the mechanism and timing of A3 gene expansion and into the functional modularity of these genes, we analyzed the genomic sequences, expressed cDNAs and activities of the full A3 repertoire of three artiodactyl lineages: sheep, cattle and pigs. 相似文献124.
Seventy-eight soil samples were collected from the various locations in the vicinity of Kaziranga National Park (Assam), India, during April to October 2009 and screened for the presence of keratinophilic fungi using the hair baiting techniques for isolation. Thirty-nine isolates were recovered and identified by recognition of their macro- and micromorphological features. Their identification was also confirmed by the BLAST search of sequences of the ITS1-5.8S-ITS2 rDNA region against the NCBI/GenBank data and compared with deposited sequences for identification purpose. Eleven species related to seven genera were recorded viz. Aphanoascus durus (1.28%), Arthroderma tuberculatum (3.84%), Arthroderma corniculatum (1.28%), Chrysosporium indicum (16.66%), C. tropicum (3.84%), Ctenomyces serratus (5.12%), Keratinophyton punsolae (1.28%), Microsporum appendiculatum (1.28%), Microsporum gypseum complex (11.53%), Trichophyton mentagrophytes (11.28%) and T. terrestre (2.56%). 相似文献
125.
Introgression of Pi2 and Pi5 Genes for Blast (Magnaporthe oryzae) Resistance in Rice and Field Evaluation of Introgression Lines for Resistance and Yield Traits
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Prashanthi Sagar KrishnaMurthy Dnyaneshwar B. Deshmukh Kakanur Jagadeesh Yashvanth Kumar Santosh Patil Shridevi Jakkeral Gangappa Hanamaratti Nemappa Uday D. Singh Mukund Variar Rajeev Rathour Gopalakrishnan Subbaiyan Ashok K. Singh Tilak Raj Sharma 《Journal of Phytopathology》2017,165(6):397-405
Blast caused by Magnaporthe oryzae is the most devastating disease causing significant loss in rice production. The destructive nature of the disease is mainly due to the genetic plasticity of M. oryzae which complicates the breeding strategies. Blast can be effectively managed by the deployment of R genes. In this study, broad‐spectrum blast resistance genes Pi2 and Pi5 were introgressed independently into popular but blast susceptible rice variety, Samba Mahsuri (BPT5204) by applying marker‐assisted backcross breeding approach. Tightly linked markers AP5930 for Pi2 and 40N23r for Pi5 gene were used in foreground selection. Background selection helped to identify the lines with maximum recovery of recurrent parent genome (RPG). The RPG recovery in Pi2 introgression lines was up to 90.17 and 91.46% in Pi5 lines. Homozygous introgression lines in BC3F4 generation carrying Pi2 and Pi5 gene were field evaluated for blast resistance, yield per se and yield‐related traits. The lines showed resistance to leaf and neck blast in multilocation field evaluation. Improved BPT5204 lines with improvement for blast resistance were on par with original BPT5204 in terms of grain yield and grain features. 相似文献
126.
Nup358 binds to AGO proteins through its SUMO‐interacting motifs and promotes the association of target mRNA with miRISC
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127.
A thermophilic bacterial strain, Streptomyces thermonitrificans, produced high levels of extracellular deoxyribonuclease (DNase) when grown on NBG medium (containing 1% peptone, 0.3% beef
extract, 1% glucose and 0.5% NaCl). Maximum DNase activity (140 U ml−1) was obtained, in 24 h, when the culture was grown on modified NBG medium (containing 1.3% beef extract, 1% glucose, 0.5%
NaCl and 50 μM Mn2+ at 45°C. The crude enzyme showed higher activity on native DNA than on sonicated and heat denatured DNA. Moreover, addition
of Mn2+ in the assay mixture resulted in a significant stimulation (10–15 fold) of the enzyme activity.
Received 24 November 1998/ Accepted in revised form 25 April 1999 相似文献
128.
Potential of DNA markers in detecting divergence and in analysing heterosis in Indian elite chickpea cultivars 总被引:2,自引:0,他引:2
V. J. Sant A. G. Patankar N. D. Sarode L. B. Mhase M. N. Sainani R. B. Deshmukh P. K. Ranjekar V. S. Gupta 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,98(8):1217-1225
Molecular markers such as RAPDs and microsatellites were used to study genetic diversity in 29 elite Indian chickpea genotypes.
In general, microsatellites were more efficient than the RAPD markers in detecting polymorphism in these genotypes. Among
the various microsatellites, (AAC)5, (ACT)5, (AAG)5 and (GATA)4 were able to differentiate all 29 chickpea cultivars. The mean value of probability of identical match by chance was 2.32×10-25 using DraI-(ACT)5, TaqI-(AAC)5, TaqI-(AAG)5 and TaqI-(GATA)4 enzyme-probe combinations. The dendrogram, constructed on the basis of similarity index values, grouped the chickpea genotypes
into five main clusters with 8 cultivars genetically distant and outgrouped from the main clusters. To investigate if DNA
markers are useful in predicting F1 performance and heterosis in chickpea, we crossed 8 genotypes having important agronomic characters in a diallel set. The
F1s and their parents in the diallel set were analysed for agronomic traits for better parent and midparent heterosis. Heterosis
was found to be much higher for yield than for yield components that fit a multiplicative model. The analysis of genetic divergence
using D2 statistics clustered the 8 cultivars into two groups. Although molecular marker-based genetic distance did not linearly correlate
to heterosis, two heterotic groups could be identified on the basis of the general marker heterozygosity.
Received: 1 August 1998 / Accepted: 29 September 1998 相似文献
129.
Synthesis and degradation of polyphosphoinositides in a rat brain synaptosome preparation were depressed by phenobarbital. Phosphatidylinositol-4-phosphate kinase (PIP-kinase), the enzyme which synthesizes phosphatidylinositol-4,5-bisphosphate (PIP2) was most strongly affected (50% inhibition at 3 mM phenobarbital); phosphatidylinositol (PI-kinase) followed (50% at 15 mM). The phosphoesterases were less sensitive: PIP-monoesterase (50% at 39 mM), PIP2-monoesterase (at 47 mM), and, least inhibited, PIP-diesterase (50% at 65 mM) and PIP2-diesterase (at 68 mM). Phenobarbital by inhibiting PIP-kinase may reduce the membrane concentration of PIP2 and thus dampen the stimulus-response which leads to the hydrolysis of PIP2 and the formation of the second messenger, inositol-1,4,5-trisphosphate (IP3), involved in mobilization of intracellular Ca2+. 相似文献
130.
D Joshi-Banka S Deshmukh S P Modak 《The International journal of developmental biology》2001,45(5-6):767-770
In the area pellucida of the chick gastrula, the Hensen's node (HN) graft must contact the competent ectoblast for at least 4 h to promote neural induction. When we removed the grafted HN after 1 to 3 h and replaced it by a non-inducing post nodal (PN) fragment, a 1-2 h contact with HN was found to be sufficient to promote neural induction. When HN graft was removed after 3 or 4 h and replaced by PN, the neural inductive response was substantially improved towards formation of archencephalic structures. Thus, our results indicate that neural induction takes place in two steps. In the first step, a contact with HN for 1-2 h is sufficient to transferthe inductive signal which is stabilized through a second step involving continued cell-cell contact with even non-inducing PN mesoblast. 相似文献