全文获取类型
收费全文 | 524篇 |
免费 | 64篇 |
专业分类
588篇 |
出版年
2022年 | 3篇 |
2021年 | 5篇 |
2020年 | 4篇 |
2019年 | 6篇 |
2018年 | 11篇 |
2017年 | 9篇 |
2016年 | 10篇 |
2015年 | 19篇 |
2014年 | 18篇 |
2013年 | 24篇 |
2012年 | 28篇 |
2011年 | 36篇 |
2010年 | 20篇 |
2009年 | 24篇 |
2008年 | 19篇 |
2007年 | 14篇 |
2006年 | 17篇 |
2005年 | 23篇 |
2004年 | 21篇 |
2003年 | 12篇 |
2002年 | 24篇 |
2001年 | 22篇 |
2000年 | 27篇 |
1999年 | 18篇 |
1998年 | 9篇 |
1997年 | 8篇 |
1996年 | 4篇 |
1995年 | 8篇 |
1994年 | 6篇 |
1993年 | 4篇 |
1992年 | 13篇 |
1991年 | 12篇 |
1990年 | 11篇 |
1989年 | 8篇 |
1988年 | 17篇 |
1987年 | 5篇 |
1986年 | 6篇 |
1985年 | 8篇 |
1984年 | 3篇 |
1982年 | 4篇 |
1977年 | 5篇 |
1976年 | 4篇 |
1974年 | 2篇 |
1971年 | 2篇 |
1970年 | 2篇 |
1969年 | 8篇 |
1967年 | 2篇 |
1930年 | 3篇 |
1928年 | 4篇 |
1926年 | 3篇 |
排序方式: 共有588条查询结果,搜索用时 0 毫秒
471.
J. G. Mina Y. Okada N. K. Wansadhipathi-Kannangara S. Pratt H. Shams-Eldin R. T. Schwarz P. G. Steel T. Fawcett P. W. Denny 《Plant molecular biology》2010,73(4-5):399-407
Sphingolipids are key components of eukaryotic plasma membranes that are involved in many functions, including the formation signal transduction complexes. In addition, these lipid species and their catabolites function as secondary signalling molecules in, amongst other processes, apoptosis. The biosynthetic pathway for the formation of sphingolipid is largely conserved. However, unlike mammalian cells, fungi, protozoa and plants synthesize inositol phosphorylceramide (IPC) as their primary phosphosphingolipid. This key step involves the transfer of the phosphorylinositol group from phosphatidylinositol (PI) to phytoceramide, a process catalysed by IPC synthase in plants and fungi. This enzyme activity is at least partly encoded by the AUR1 gene in the fungi, and recently the distantly related functional orthologue of this gene has been identified in the model plant Arabidopsis. Here we functionally analysed all three predicted Arabidopsis IPC synthases, confirming them as aureobasidin A resistant AUR1p orthologues. Expression profiling revealed that the genes encoding these orthologues are differentially expressed in various tissue types isolated from Arabidopsis. 相似文献
472.
Peethambaran Divya Bijesh Puthusseri Denny Joseph Kollareth Manual Mohammed Azharuddin Savanur 《Molecular and cellular biochemistry》2017,434(1-2):105-111
Gastrin-releasing peptide (GRP) plays a major role in the development and maintenance of lung epithelial cells by promoting cell division, whereas its suppression causes growth arrest and apoptosis. The present study shows that human bronchial epithelial BEAS-2B cells challenged with lipopolysaccharide (LPS), an endotoxin from gram-negative bacteria, downregulated GRP expression and induced apoptosis via upregulation of p53 and active caspase-3, signifying the importance of GRP in lung epithelial cell survival. However, in the presence of epigallocatechin-3-gallate (EGCG), a polyphenol in green tea, BEAS-2B cells resisted LPS-induced apoptosis and restored the expression of GRP and its downstream effectors such as epidermal growth factor receptor and NF-κB, as analysed by immunoblotting and qPCR. Based on our findings, we objectify that cytoprotective functions of EGCG, via upregulation of GRP in cells challenged with LPS, are novel and can be further explored in a therapeutic point of view for diseases such as septic shock. 相似文献
473.
474.
Laboratory soil bioassays were performed at economic field rates for in-furrow (3.85 x 10(6)spores/g dry soil) and broadcast (3.85 x 10(5)spores/g dry soil) applications with three isolates of Metarhizium anisopliae (F52, ATCC62176, and ARSEF5520) and one isolate of Beauveria bassiana (GHA). All isolates tested were infective to second instar Delia radicum (L.). The conditionally registered M. anisopliae isolate (F52) performed best killing an average of 85 and 72% of D. radicum larvae at the high and low concentration, respectively. The mean LC50 and LC95 of F52 against second instar D. radicum was 2.7 x 10(6) and 1.8 x 10(8)spores/g dry soil, respectively. The use of F52 in an integrated management program is discussed. 相似文献
475.
476.
Justin Pollara Erin McGuire Genevieve G. Fouda Wes Rountree Josh Eudailey R. Glenn Overman Kelly E. Seaton Aaron Deal R. Whitney Edwards Gerald Tegha Deborah Kamwendo Jacob Kumwenda Julie A. E. Nelson Hua-Xin Liao Christie Brinkley Thomas N. Denny Christina Ochsenbauer Sascha Ellington Caroline C. King Denise J. Jamieson Charles van der Horst Athena P. Kourtis Georgia D. Tomaras Guido Ferrari Sallie R. Permar 《Journal of virology》2015,89(19):9952-9961
477.
Anna C. Giddens Swarna A. Gamage Jackie D. Kendall Woo-Jeong Lee Bruce C. Baguley Christina M. Buchanan Stephen M.F. Jamieson James M.J. Dickson Peter R. Shepherd William A. Denny Gordon W. Rewcastle 《Bioorganic & medicinal chemistry》2019,27(8):1529-1545
Replacing one of the morpholine groups of the phosphatidylinositol 3-kinase (PI3K) inhibitor ZSTK474 with a variety of sulfonamide-linked solubilizing substituents produced a new class of active and potent PI3Kα inhibitors, with several derivatives demonstrating high PI3Kα enzyme potency and good cellular potency in two human derived cell lines. The overall results suggest a preference for linear and somewhat flexible solubilizing functions. From this series, compound 16, also known as SN32976, was selected for advanced preclinical evaluation. 相似文献
478.
Song-Can Chen Jiaheng Ji Denny Popp Ulrike Jaekel Hans-Hermann Richnow Stefan M. Sievert Florin Musat 《Environmental microbiology》2022,24(4):1964-1976
The metabolic potential of the sulfate-reducing bacterium Desulfosarcina sp. strain BuS5, currently the only pure culture able to oxidize the volatile alkanes propane and butane without oxygen, was investigated via genomics, proteomics and physiology assays. Complete genome sequencing revealed that strain BuS5 encodes a single alkyl-succinate synthase, an enzyme which apparently initiates oxidation of both propane and butane. The formed alkyl-succinates are oxidized to CO2 via beta oxidation and the oxidative Wood–Ljungdahl pathways as shown by proteogenomics analyses. Strain BuS5 conserves energy via the canonical sulfate reduction pathway and electron bifurcation. An ability to utilize long-chain fatty acids, mannose and oligopeptides, suggested by automated annotation pipelines, was not supported by physiology assays and in-depth analyses of the corresponding genetic systems. Consistently, comparative genomics revealed a streamlined BuS5 genome with a remarkable paucity of catabolic modules. These results establish strain BuS5 as an exceptional metabolic specialist, able to grow only with propane and butane, for which we propose the name Desulfosarcina aeriophaga BuS5. This highly restrictive lifestyle, most likely the result of habitat-driven evolutionary gene loss, may provide D. aeriophaga BuS5 a competitive edge in sediments impacted by natural gas seeps. Etymology: Desulfosarcina aeriophaga, aério (Greek): gas; phágos (Greek): eater; D. aeriophaga: a gas eating or gas feeding Desulfosarcina. 相似文献
479.
480.