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141.
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143.
Denisa Blažková 《Folia Geobotanica》1971,6(3):271-279
Junco inflexi-Menthetum longifoliae stellt auf dem Gebiet der Tschechoslowakei eine sowohl floristisch als auch ökologisch zeimlich gut ausgeprägte Assoziation dar. Es trägt den kennzeichnenden Charakter der Kontaktgesellschaften mit Bindung an nasse bis wechselfeuchtige Böden, deren Sorptionskomplex mit Ionen zweiwertiger Basen gesättigt ist. Es sind zwei Subassoziationen angeführt:Junco inflexi-Menthetum longifoliae typicum undJunco inflexi-Menthetum longifoliae caricetosum. An analogen Standorten mit saureren Böden und nicht völlig gesättigtem Sorptionskomplex entwickelt sich die AssoziationEpilobio-Juncetum (effusi). 相似文献
144.
Denisa Harvanová Ján Rosocha Du?an Bako? Róbert ?vihla Gabriel Va?ko Slavomír Horňák Valent Ledecky Sylvia Gromo?ová Peter Cibur Richard Ra?i 《Biologia》2009,64(5):1032-1038
Rabbit chondrocytes were cultivated in vitro using the collagen/hyaluronan membrane. The membrane did not show any adverse effects on chondrocyte viability during in vitro cultivation. The inoculated cells grew without any negative changes. According to the histochemical analyses: (i) hematoxylin
and eosin; (ii) safranin O; and (iii) rabbit anti-human collagen type II staining, the rabbit chondrocytes maintained their
morphology and phenotype during in vitro cultivation. The collagen/hyaluronan membrane became more stable and stiffer after long time cultivation. The proliferation
of the chondrocytes stabilised the structure of the membrane. The collagen/hyaluronan membrane is suitable material for the
chondrocyte growth and could provide functional tissue-engineered scaffold for cartilage repair. 相似文献
145.
Effect of PRP and PPP on proliferation and migration of human chondrocytes and synoviocytes in vitro
Judita Amrichová Tímea Špaková Ján Rosocha Denisa Harvanová Darina Bačenková Marek Lacko Slavomír Horňák 《Central European Journal of Biology》2014,9(2):139-148
Cartilage tissue engineering can provide substantial relief to people suffering from degenerative cartilage disease, such as osteoarthritis. The autologous platelet-rich plasma (PRP) application appears to improve cartilage healing due to its ability to positively influence cellular mechanisms, mainly in cells from synovium and cartilage. Primary cultures of human synovial fluid stem cells (synoviocytes, SCs) and chondrocytes (CCs) were exposed to various concentrations of non-activated PRP and plateletpoor plasma (PPP) prepared by apheresis. Cell proliferation and migration were evaluated in real-time with the non-invasive xCELLigence System. It was found that PRP had a similar effect on the growth of cells as fetal bovine serum (FBS). Surprisingly, our proliferation assay results indicated that 50% PPP had the largest effect on both cell types, with a statistically significant increase in cell number (P<0.001) compared to the (0% FBS) in vitro control. The migratory ability of SCs was significantly enhanced with 10% PRP and 0.8% hyaluronic acid (HA). HA also augmented migration of CCs. In summary, these results demonstrate that directed cell proliferation and migration are inducible in human articular CCs and SCs, and that both platelet-derived fractions may exert a positive effect and modulate several cell responses that are potentially involved in tissue integration during cartilage repair. 相似文献