Junco inflexi-Menthetum longifoliae Lohm. 1953 undEpilobio-Juncetum (effusi) Oberd. 1957 in der Tschechoslowakei

Junco inflexi-Menthetum longifoliae stellt auf dem Gebiet der Tschechoslowakei eine sowohl floristisch als auch ökologisch zeimlich gut ausgeprägte Assoziation dar. Es trägt den kennzeichnenden Charakter der Kontaktgesellschaften mit Bindung an nasse bis wechselfeuchtige Böden, deren Sorptionskomplex mit Ionen zweiwertiger Basen gesättigt ist. Es sind zwei Subassoziationen angeführt:Junco inflexi-Menthetum longifoliae typicum undJunco inflexi-Menthetum longifoliae caricetosum. An analogen Standorten mit saureren Böden und nicht völlig gesättigtem Sorptionskomplex entwickelt sich die AssoziationEpilobio-Juncetum (effusi).     

Collagen/hyaluronan membrane as a scaffold for chondrocytes cultivation

Rabbit chondrocytes were cultivated in vitro using the collagen/hyaluronan membrane. The membrane did not show any adverse effects on chondrocyte viability during in vitro cultivation. The inoculated cells grew without any negative changes. According to the histochemical analyses: (i) hematoxylin and eosin; (ii) safranin O; and (iii) rabbit anti-human collagen type II staining, the rabbit chondrocytes maintained their morphology and phenotype during in vitro cultivation. The collagen/hyaluronan membrane became more stable and stiffer after long time cultivation. The proliferation of the chondrocytes stabilised the structure of the membrane. The collagen/hyaluronan membrane is suitable material for the chondrocyte growth and could provide functional tissue-engineered scaffold for cartilage repair.     

Effect of PRP and PPP on proliferation and migration of human chondrocytes and synoviocytes in vitro

Cartilage tissue engineering can provide substantial relief to people suffering from degenerative cartilage disease, such as osteoarthritis. The autologous platelet-rich plasma (PRP) application appears to improve cartilage healing due to its ability to positively influence cellular mechanisms, mainly in cells from synovium and cartilage. Primary cultures of human synovial fluid stem cells (synoviocytes, SCs) and chondrocytes (CCs) were exposed to various concentrations of non-activated PRP and plateletpoor plasma (PPP) prepared by apheresis. Cell proliferation and migration were evaluated in real-time with the non-invasive xCELLigence System. It was found that PRP had a similar effect on the growth of cells as fetal bovine serum (FBS). Surprisingly, our proliferation assay results indicated that 50% PPP had the largest effect on both cell types, with a statistically significant increase in cell number (P<0.001) compared to the (0% FBS) in vitro control. The migratory ability of SCs was significantly enhanced with 10% PRP and 0.8% hyaluronic acid (HA). HA also augmented migration of CCs. In summary, these results demonstrate that directed cell proliferation and migration are inducible in human articular CCs and SCs, and that both platelet-derived fractions may exert a positive effect and modulate several cell responses that are potentially involved in tissue integration during cartilage repair.