全文获取类型
收费全文 | 24722篇 |
免费 | 2287篇 |
国内免费 | 2394篇 |
专业分类
29403篇 |
出版年
2024年 | 77篇 |
2023年 | 363篇 |
2022年 | 845篇 |
2021年 | 1318篇 |
2020年 | 953篇 |
2019年 | 1120篇 |
2018年 | 1032篇 |
2017年 | 782篇 |
2016年 | 1133篇 |
2015年 | 1549篇 |
2014年 | 1977篇 |
2013年 | 1903篇 |
2012年 | 2242篇 |
2011年 | 1989篇 |
2010年 | 1239篇 |
2009年 | 1159篇 |
2008年 | 1369篇 |
2007年 | 1194篇 |
2006年 | 976篇 |
2005年 | 801篇 |
2004年 | 745篇 |
2003年 | 651篇 |
2002年 | 581篇 |
2001年 | 426篇 |
2000年 | 383篇 |
1999年 | 381篇 |
1998年 | 264篇 |
1997年 | 242篇 |
1996年 | 213篇 |
1995年 | 154篇 |
1994年 | 163篇 |
1993年 | 113篇 |
1992年 | 162篇 |
1991年 | 142篇 |
1990年 | 93篇 |
1989年 | 80篇 |
1988年 | 75篇 |
1987年 | 71篇 |
1986年 | 49篇 |
1985年 | 63篇 |
1984年 | 44篇 |
1983年 | 35篇 |
1982年 | 22篇 |
1981年 | 16篇 |
1980年 | 22篇 |
1979年 | 23篇 |
1978年 | 16篇 |
1977年 | 16篇 |
1974年 | 16篇 |
1970年 | 16篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
141.
Ding M Shen K 《BioEssays : news and reviews in molecular, cellular and developmental biology》2008,30(11-12):1075-1083
The ubiquitin proteasome system is a potent regulatory mechanism used to control protein stability in numerous cellular processes, including neural development. Many neurodegenerative diseases are featured by the accumulation of UPS-associated proteins, suggesting the UPS dysfunction may be crucial for pathogenesis. Recent experiments have highlighted the UPS as a key player during synaptic development. Here we summarize recent discoveries centered on the role of the UPS in synapse remodeling and draw attention to the potential link between the synaptic UPS dysfunction and the pathology of neurodegenerative diseases. 相似文献
142.
Jeemeng Lao Ai Oikawa Jennifer R. Bromley Peter McInerney Anongpat Suttangkakul Andreia M. Smith‐Moritz Hector Plahar Tsan‐Yu Chiu Susana M. González Fernández‐Niño Berit Ebert Fan Yang Katy M. Christiansen Sara F. Hansen Solomon Stonebloom Paul D. Adams Pamela C. Ronald Nathan J. Hillson Masood Z. Hadi Miguel E. Vega‐Sánchez Dominique Loqué Henrik V. Scheller Joshua L. Heazlewood 《The Plant journal : for cell and molecular biology》2014,79(3):517-529
The glycosyltransferases (GTs) are an important and functionally diverse family of enzymes involved in glycan and glycoside biosynthesis. Plants have evolved large families of GTs which undertake the array of glycosylation reactions that occur during plant development and growth. Based on the Carbohydrate‐Active enZymes (CAZy) database, the genome of the reference plant Arabidopsis thaliana codes for over 450 GTs, while the rice genome (Oryza sativa) contains over 600 members. Collectively, GTs from these reference plants can be classified into over 40 distinct GT families. Although these enzymes are involved in many important plant specific processes such as cell‐wall and secondary metabolite biosynthesis, few have been functionally characterized. We have sought to develop a plant GTs clone resource that will enable functional genomic approaches to be undertaken by the plant research community. In total, 403 (88%) of CAZy defined Arabidopsis GTs have been cloned, while 96 (15%) of the GTs coded by rice have been cloned. The collection resulted in the update of a number of Arabidopsis GT gene models. The clones represent full‐length coding sequences without termination codons and are Gateway® compatible. To demonstrate the utility of this JBEI GT Collection, a set of efficient particle bombardment plasmids (pBullet) was also constructed with markers for the endomembrane. The utility of the pBullet collection was demonstrated by localizing all members of the Arabidopsis GT14 family to the Golgi apparatus or the endoplasmic reticulum (ER). Updates to these resources are available at the JBEI GT Collection website http://www.addgene.org/ . 相似文献
143.
144.
Cell death induced by Pteris semipinnata L. is associated with p53 and oxidant stress in gastric cancer cells 总被引:4,自引:0,他引:4
In this study, we demonstrated that Ent-11alpha-hydroxy-15-oxo-kaur-16-en-19-oic-acid (5F) had stronger cytotoxicity against MKN-45, a gastric cancer cell line bearing wild-type p53 than MKN-28, another gastric cancer cell line containing missense mutation in p53. The rapid increase of ROS level was involved in the mechanism of cytotoxicity. Classical features of apoptosis induced by 5F were observed in MKN-45 cells only or more significant in MKN-45 cells than MKN-28 cells. Translocation of Bax from cytosol to mitochondria, reduction of delta psi m and DNA fragmentation were induced by 5F in the p53-dependent manner. We conclude that the expression of Bax and its downstream molecules requires the presentation of a wild-type p53 in the cells treated by 5F. 相似文献
145.
146.
Dragon's blood may have radioprotective effects in radiation-induced rat brain injury 总被引:2,自引:0,他引:2
147.
148.
149.
Yafei Tian Yongping Zhang Shaoyan Hu Lilan Yao Yijian Zhu Shenglong Qiao Daru Lu Junjie Fan 《Blood and Genomics》2021,664(1):63-67
MYH9-related diseases (MYH9-RD) are a group of autosomal dominant diseases caused by mutations in the MYH9 gene, which are featured by thrombocytopenia, giant platelets and granulocyte cytoplasmic inclusion bodies. MYH9-RD patients generally suffer from bleeding syndromes, progressive kidney disease, deafness, or cataracts. Here, we reported on a case of MYH9-RD. A novel heterozygous mutation of MYH9 (c.2344-2345delGTinsTA, p.T782Y) was discovered by targeted sequencing technology. Immunofluorescence analysis of neutrophils confirmed abnormal aggregation of MYH9 protein. The results of this study should expand the MYH9 gene mutation spectrum and provide reference for subsequent researchers and genetic counseling. 相似文献
150.