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31.
Arbuscular mycorrhizal fungi enhance fruit growth and quality of chile ancho (Capsicum annuum L. cv San Luis) plants exposed to drought 总被引:1,自引:0,他引:1
Mena-Violante HG Ocampo-Jiménez O Dendooven L Martínez-Soto G González-Castañeda J Davies FT Olalde-Portugal V 《Mycorrhiza》2006,16(4):261-267
The effect of arbuscular mycorrhizal fungi (AMF) and drought on fruit quality was evaluated in chile ancho (Capsicum annuum L. cv San Luis). AMF treatments were (1) Glomus fasciculatum (AMFG), (2) a fungal species consortium from the forest “Los Tuxtla” in Mexico (AMFT), (3) a fungal species consortium from the Sonorian desert in Mexico (AMFD), and (4) a noninoculated control (NAMF). Plants were exposed to a 26-day drought cycle. Fruit quality was determined by measuring size (length, width, and pedicel length), color, chlorophyll, and carotenoid concentration. Under nondrought conditions, AMFG produced fruits that were 13% wider and 15% longer than the NAMF treatment. Under nondrought conditions, fruit fresh weight was 25% greater in the AMFG treatment compared to the NAMF. Under drought, fruits in the AMFT and AMFD treatments showed fresh weights similar to those in the NAMF treatment not subjected to drought. Fruits of the AMFG treatment subjected to drought showed the same color intensity and chlorophyll content as those of the nondroughted NAMF treatment and carotenoid content increased 1.4 times compared to that in the NAMF not exposed to drought. It is interesting to note that fruits in the AMFD treatment subjected to drought and the NAMF treatment not exposed to drought reached the same size. AMFD treatment increased the concentration of carotenes (1.4 times) under nondrought conditions and the concentration of xanthophylls (1.5 times) under drought when compared to the nondroughted NAMF treatment. 相似文献
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33.
Alferso C. Abrahams Sayed M. Habib Amélie Dendooven Bruce L. Riser Jan Willem van der Veer Raechel J. Toorop Michiel G. H. Betjes Marianne C. Verhaar Christopher J. E. Watson Tri Q. Nguyen Walther H. Boer 《PloS one》2014,9(11)
Introduction
Encapsulating peritoneal sclerosis (EPS) is a devastating complication of peritoneal dialysis (PD). The pathogenesis is not exactly known and no preventive strategy or targeted medical therapy is available. CCN2 has both pro-fibrotic and pro-angiogenic actions and appears an attractive target. Therefore, we studied peritoneal expression of CCN2, as well as TGFβ1 and VEGF, in different stages of peritoneal fibrosis.Materials and methods
Sixteen PD patients were investigated and compared to 12 hemodialysis patients and four pre-emptively transplanted patients. Furthermore, expression was investigated in 12 EPS patients in comparison with 13 PD and 12 non-PD patients without EPS. Peritoneal tissue was taken during kidney transplantation procedure or during EPS surgery. In a subset of patients, CCN2 protein levels in peritoneal effluent and plasma were determined. Samples were examined by qPCR, histology, immunohistochemistry, and ELISA.Results
Peritoneal CCN2 expression was 5-fold higher in PD patients compared to pre-emptively transplanted patients (P<0.05), but did not differ from hemodialysis patients. Peritoneal expression of TGFβ1 and VEGF were not different between the three groups; neither was peritoneal thickness. Peritoneum of EPS patients exhibited increased expression of CCN2 (35-fold, P<0.001), TGFβ1 (24-fold, P<0.05), and VEGF (77-fold, P<0.001) compared to PD patients without EPS. In EPS patients, CCN2 protein was mainly localized in peritoneal endothelial cells and fibroblasts. CCN2 protein levels were significantly higher in peritoneal effluent of EPS patients compared to levels in dialysate of PD patients (12.0±4.5 vs. 0.91±0.92 ng/ml, P<0.01), while plasma CCN2 levels were not increased.Conclusions
Peritoneal expression of CCN2, TGFβ1, and VEGF are significantly increased in EPS patients. In early stages of peritoneal fibrosis, only CCN2 expression is slightly increased. Peritoneal CCN2 overexpression in EPS patients is a locally driven response. The potential of CCN2 as biomarker and target for CCN2-inhibiting agents to prevent or treat EPS warrants further study. 相似文献34.
Betancur-Galvis LA Carrillo H Luna-Guido M Marsch R Dendooven L 《International journal of phytoremediation》2012,14(8):741-753
Remediation of polycyclic aromatic hydrocarbons (PAHs) contaminated alkaline saline soil with phreatophyte or "water loving plants" was investigated by spiking soil from the former lake Texcoco with 100 mg phenanthrene (Phen) kg(-1) soil, 120 mg anthracene (Ant)kg(-1) soil and 45 mg benzo(a)pyrene (BaP) kg(-1) soil and vegetating it with Athel tamarisk (Tamarix aphylla L Karst.). The growth of the Athel tamarisk was not affected by the PAHs. In soil cultivated with Athel tamarisk, the leaching of PAHs to the 32-34 cm layer decreased 2-fold compared to the uncultivated soil. The BaP concentration decreased to 39% of the initial concentration at a distance smaller than 3 cm from the roots and to 45% at a distance larger than 3cm, but 59% remained in unvegetated soil after 240 days. Dissipation of Ant and Phen decreased with depth, but not BaP. The biodegradation of PAHs was affected by their chemical properties and increased in the presence of T. aphylla, but decreased with depth. 相似文献
35.
Vázquez Núñez E García Gaytán A Luna-Guido M Marsch R Dendooven L 《Biodegradation》2009,20(2):191-198
In a previous study, remediation of anthracene from soil was faster in the top 0–2 cm layer than in the lower soil layers.
It was not clear whether this faster decrease was due to biotic or abiotic processes. Anthracene-contaminated soil columns
were covered with black or transparent perforated polyethylene so that aeration occurred but that fluctuations in water content
were minimal and light could reach (LIGHT treatment) or not reach the soil surface (DARK treatment), or left uncovered so
that soil water content fluctuate and light reached the soil surface (OPEN treatment). The amount of anthracene, microbial
biomass C, and microbial activity as reflected by the amount of CO2 produced within 3 days were determined in the 0–2 cm, 2–8 cm, and 8–15 cm layer after 0, 3, 7, 14, and 28 days. In the 0–2 cm
layer of the OPEN treatment, 17% anthracene remained, 48% in the LIGHT treatment and 61% in the DARK treatment after 28 days.
In the 2–8 cm and 8–15 cm layer, treatment had no significant effect on the dissipation of anthracene from soil after 14 and
28 days. It was found that light and fluctuations in water content stimulated the removal of anthracene from the top 0–2 cm
soil layer, but not from the lower soil layers. It can be speculated that covering contaminated soil or pilling it up will
inhibit the dissipation of the contaminant. 相似文献
36.
Aggregation and C and N contents of soil organic matter fractions in a permanent raised-bed planting system in the Highlands of Central Mexico 总被引:2,自引:0,他引:2
Kelly Lichter Bram Govaerts Johan Six Ken D. Sayre Jozef Deckers Luc Dendooven 《Plant and Soil》2008,305(1-2):237-252
Permanent raised bed planting with crop residue retention is a form of conservation agriculture that has been proposed as
an alternative to conventional tillage for wheat production systems in the Central Highlands of Mexico. A field experiment
comparing permanent and tilled raised beds with different residue management under rainfed conditions was started at El Batán
(State of Mexico, Mexico) in 1999. The percentage of small and large macroaggregates and mean weight diameter (MWD) was significantly
larger in permanent raised beds compared to conventionally tilled raised beds both with full crop residue retention (average
for maize and wheat), while the percentages free microaggregates was lower. The percentages of small and large macroaggregates
and mean weight diameter (MWD) was significantly larger in permanent raised beds with residue retention compared to permanent
raised beds with removal of the residue (average for maize and wheat), while the percentages free microaggregates and silt
and clay fraction was lower. Cultivation of maize significantly reduced the large macroaggregates, while wheat reduced the
silt and clay fraction (average over all systems). Cultivation of maize reduced the C and N content of the free microaggregates
compared to soil cultivated with wheat, while removal of plant residue reduced the C and N content of the silt and clay fraction
compared to soil where residue was retained. The C and N content of the coarse particulate organic matter (cPOM) and microaggregates
within the macroaggregates was significantly larger in permanent raised beds compared to conventionally tilled raised beds
both with full residue retention, while C and N content of the cPOM was significantly lower when residue was removed or partially
removed compared to the soil where the residue was retained. The δ
13C ‰ signatures of the macroaggregates, microaggregates, the silt and clay fraction, cPOM and microaggregates within the macroaggregates
were not affected by tillage or residue management when wheat was the last crop, but removal of residue reduced the δ
13C ‰ signatures of the macro-, microaggregates and microaggregates within the macroaggregates significantly compared to soil
where the residue was retained. Retaining only 30–50% of the organic residue still improved the soil structure considerably
compared to plots where it was removed completely. Permanent raised beds without residue retention, however, is a practice
leading to soil degradation.
Kelly Lichter and Bram Govaerts contributed equally to this publication. 相似文献
37.
Selective production of bikaverin in a fluidized bioreactor with immobilized Gibberella fujikuroi 总被引:1,自引:0,他引:1
Escamilla-Silva Eleazar Poggi-Varaldo Héctor De la Torre-Martínez M. Mayra Sanchez Cornejo M.A. Guadalupe Dendooven Luc 《World journal of microbiology & biotechnology》2001,17(5):469-474
The best culture medium composition for the production of bikaverin by Gibberella fujikuroi in shake-flasks, i.e. 100 g glucose l–1; 1 g NH4Cl l–1; 2 g rice flour l–1; 5 g KH2PO4 l–1 and 2.5 g MgSO4 l–1, was obtained through a fractional factorial design and then scaled-up to a fluidized bioreactor. The effects of carbon and nitrogen concentrations, inoculum size, aeration, flow rate and bead sizes on batch bikaverin production using immobilized G. fujikuroi in a fluidized bioreactor were determined by an orthogonal experimental design. Concentrations of up to 6.83 g bikaverin l–1 were obtained when the medium contained 100 g glucose l–1 and 1 g NH4Cl l–1 with an inoculum ratio of 10% v/v, an aeration rate of 3 volumes of air per volume of medium min–1, and a bead size of 3 mm. Based on dry weight, the bikaverin production was 30–100 times larger than found in submerged culture and approximately three times larger than reported for solid substrate fermentation. 相似文献
38.
Silva EM Gutierrez GF Dendooven L Jiménez I H Ochoa-Tapia JA 《Biotechnology progress》2001,17(1):95-103
Several models have been developed simulating O2 transfer in bioreactors, but three limitations are often found: (i) an inadequate kinetic representation of O2 consumption or wrong boundary conditions, (ii) unrealistic parameter values, and (iii) inadequate experimental systems. In our study we minimized those possible sources of error. Oxygen uptake rate, void fraction of the pellet, and external O2 mass transfer coefficient were experimentally obtained from bioreactor studies in which pellets of Gibberella fujikuroi were naturally formed. Michaelis-Menten kinetics and diffusion equations were used to describe the O2 consumption rate and to evaluate the effectiveness factor in dynamic mode. The nonlinear mathematical model proposed was solved by the orthogonal collocation technique. The O2 consumption rate in pellets of G. fujikuroi of 1.7-2.0 mm is only marginally inhibited by diffusion constraints under conditions tested. Simulation analysis showed that the effectiveness factor decreased as the Thiele modulus and pellet diameter increased. The proposed model was applied to experimental data reported for other fungal pellets and allowed to predict optimal conditions for O2 transfer into mycelial pellets. 相似文献
39.
Characteristics,and carbon and nitrogen dynamics in soil irrigated with wastewater for different lengths of time 总被引:17,自引:0,他引:17
Ramirez-Fuentes E Lucho-constantino C Escamilla-Silva E Dendooven L 《Bioresource technology》2002,85(2):179-187
Irrigation of agricultural land with wastewater will increase crop production, but also heavy metal concentrations and the rate of infection of farmers with pathogens. The risks associated with the use of wastewater are reduced by treating the wastewater, but treatment also reduces organic material, phosphorus and inorganic N for crops. We investigated characteristics, e.g. heavy metal concentrations, of soils of the valley of the Mezquital (Mexico) irrigated with waste from Mexico City water since 1912, 1925, 1965, 1976, 1996 or 1997, or not irrigated at all, and dynamics of C and N when soil was amended with wastewater or drainage water. Concentrations of total Mg, Hg, Mo, Ca, Cu and Cr, available concentrations of Pb, Cd and Cu increased significantly with length of irrigation (P < 0.05), but were not at hazardous concentrations. Although organic C, total N, microbial biomass C and N, and microbial activity, as witnessed by CO2 production, increased with length of irrigation, N mineralization did not. Oxidation of NO2- was inhibited and could be due to increases in salinity, toxic compounds or heavy metals. We found that N mineralization was low or absent so it will not compensate for the loss of N when the wastewater is treated and application of N fertilizer will be required to maintain the same level of crop production. The characteristics of the soils appear not to have deteriorated after years of application of wastewater, but further irrigation even with treated wastewater might increase sodicity and salinity and pose a threat to future crop production. 相似文献
40.
Bram Govaerts Nele Verhulst Ken D. Sayre Pieter De Corte Bart Goudeseune Kelly Lichter Josse Crossa Jozef Deckers Luc Dendooven 《Plant and Soil》2007,297(1-2):29-42
Subsoil constraints to root growth exacerbate frequent water and nutrient limitations to crop yields in Mediterranean-type
environments. Amelioration of subsoil constraints can relieve these limitations by opening root-access to subsoil water and
nutrients. However, decisions in subsoil amelioration are hampered by seasonally variable yield responses in these environments.
We used the APSIM model to analyse the impact of subsoil constraints on yield and yield variability. The simulated yield data
were used to calculate the financial benefits of subsoil amelioration across several scenarios. There was a strong yield-dependence
on accessible soil water governed by root depth. Root depth development was limited to a minimum of either the effect of subsoil
constraints or the weather-dependent depth of the soil wetting front. Insufficient rainfall in dry years or in a drier region
often resulted in shallow soil wetting fronts and correspondingly shallow roots even in the absence of subsoil compaction.
In these situations, there is little response to subsoil amelioration. Positive yield responses and positive financial returns
to subsoil amelioration are therefore greater in good rainfall years and are more likely in a wetter region. A yield response
to amelioration is also greater in coarser textured sand than finer textured sandy loam in an average rainfall season because
the same amount of rainfall results in a deeper wetting front in sand than in sandy loam. Hence, roots in a sand are required
to grow deeper compared to a sandy loam to access the same amount of water and therefore benefited more from subsoil amelioration
in an average rainfall year. In wet years, sands leach more nitrate than sandy loam, which decreases yields and the response
to subsoil amelioration in sands is more than in the sandy loam. Environmental threats occur along with yield loss when roots
cannot access subsoil water. These include increased nitrate leaching and deep drainage due to unused water remaining in the
soil profile. By allowing roots to access deep soil water, ameliorating subsoil is expected to yield financial gains in average
to good rainfall seasons and decrease the environmental risk of drainage and leaching loss. The financial gains are expected
to offset potential financial losses in dry and dry finish seasons especially in coarser textured soils and wetter environment.
Responsible Editor: Jan Vos. 相似文献