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31.
32.
Using the non-ionic detergent dodecyl--D-maltoside we have developed a preparative method for the isolation of the 43 kDa, 47 kDa and D1-D2-Cyt b
559 species directly from thylakoid membranes. In contrast to previous procedures the photosynthetic membrane was exposed only to one mild detergent and that resulted in more stable preparations. The isolated species were examined spectroscopically and it was found that even under these mild conditions the D1-D2-Cyt b
559 did not retain the primary quinone QA.Abbreviations PS II
Photosystem II
- CF
Coupling Factor
- LHC
Light Harvesting Complex
- MES
4-morpholine-ethanesulfonic acid
- BIS-TRIS
2-[Bis (2-hydroxyethyl) amino]-2-hydroxymethyl-propane-1,3-diol
- SMN
0.4 M sucrose/50 mM MES (pH6)/10 mM NaCl 相似文献
33.
We have investigated the levels of protein encoded by the ras oncogene in normal mouse tissues using an immunoblotting technique. We have found that heart from young or adult NIH or Balb C strains of mice contain high levels of ras protein as compared to lung, liver, spleen, kidney, brain and skeletal muscle tissues from the same animal. Our results indicate that cellular ras expression does not in every case correlate with cell proliferation. 相似文献
34.
Demetrios G. Voyiatzis 《Physiologia plantarum》1995,95(3):444-448
Olive seeds do not germinate promptly when placed under favourable conditions, which is a problem in raising young plants for breeding or experimental purposes. In a series of experiments an investigation of the role of temperature in the germination of olive embryos was conducted. Naked, unchilled olive embryos ( Olea europaea L. cv. Chalkidikis), cultured in vitro at 20°C, had a germination capacity of 73%, whereas that of embryos which had previously been chilled at 10°C for 2 or more weeks reached 96%. Intact seeds did not germinate at 20°C unless they had previously been subjected to 10°C for 3 or 4 weeks. Embryos chilled while in the intact seed and excised just before transfer to 20°C, reacted in a similar way to naked embryos, but reached their maximum germination capacity after 4 weeks at 10°C. Under constant temperature conditions the highest germination percentage of embryos was observed at 10 and 15°C and the highest germination rate at 15°C, while a moderate capacity and rate of germination occurred at 20°C, and a very low percentage and rate at 25 and 30°C. Prechilling at 10°C did not affect germination at 15°C, but improved the percentage and the rate of germination at 20, 25 and 30°C. The germination percentages of embryos chilled for 1 or 2 weeks at 10°C and then transferred to 25°C were lower than those of similarly chilled embryos transferred to 20°C. The chilling effect could not be reversed at 25°C when the embryos had been chilled for 3 or more weeks. The results show that olive seeds exhibit a state of dormancy that is caused by factors residing partly in the endosperm and partly within the embryo. 相似文献
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Georgia Demsia Dimitris Vlastos Marina Goumenou Demetrios P. Matthopoulos 《Mutation Research - Genetic Toxicology and Environmental Mutagenesis》2007,634(1-2):32-39
Imidacloprid and metalaxyl are two pesticides that are widely used in agriculture, either separately, or in combination. These agents were studied for their possible genotoxic effects with respect to the following cytogenetic end-points: (1) in vitro micronucleus (MN) formation and sister-chromatid exchange (SCE) induction in human lymphocytes and (2) in vivo micronucleus induction in polychromatic erythrocytes (PCEs) of the rat bone-marrow. The results of the MN analysis indicate that MN frequencies after treatment with both pesticides, separately or as a mixture, do not significantly differ from those in the controls except after treatment with metalaxyl alone at 50 μg/ml (p < 0.05). The results of the SCE analysis show that SCE frequencies after treatment with imidacloprid do not differ significantly from those in the controls. A statistically significant increase (p < 0.05) in SCE frequency resulted from treatments with metalaxyl at 5, 10 and 100 μg/ml and with the combination of imidacloprid and metalaxyl at 100 and 200 μg/ml. Finally, the in vivo micronucleus assay with rat bone-marrow polychromatic erythrocytes showed a statistically significant effect upon separate treatments with imidacloprid and metalaxyl at doses of 300 mg/kg body weight (b.w.) (p < 0.01) or upon combined treatment with 200 mg/Kg b.w. (p < 0.001) and 400 mg/kg b.w. (p < 0.05). 相似文献
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39.
Analysis of Expressed Sequence Tag Data and Gene Expression Profiles Involved in Conidial Germination of Fusarium oxysporum 下载免费PDF全文
40.
Kiriakos Kotzabasis Constantina Fotinou Kalliopi A. Roubelakis-Angelakis Demetrios Ghanotakis 《Photosynthesis research》1993,38(1):83-88
The three main polyamines putrescine (Put), spermidine (Spd) and spermine (Spm) were characterized by HPLC in intact spinach leaf cells, intact chloroplasts, thylakoid membranes, Photosystem II membranes, the light-harvesting complex and the PS II complex. All contain the three polyamines in various ratios; the HPLC polyamine profiles of highly resolved PS II species (a Photosystem II core and the rection center) suggest an enrichment in the polyamine Spm.Abbreviations Chl
chlorophyll
- HPLC
high performance liquid chromatography
- LHC
light-harvesting complex
- PS II
Photosystem II
- PS II-RC
Photosystem II reaction center
- Put
putrescine
- Spd
spermidine
- Spm
spermine
- 10%S-core
D1-D2-Cyt b559-47 kD-43 kD complex 相似文献