全文获取类型
收费全文 | 1404篇 |
免费 | 123篇 |
出版年
2023年 | 8篇 |
2022年 | 15篇 |
2021年 | 30篇 |
2020年 | 20篇 |
2019年 | 24篇 |
2018年 | 26篇 |
2017年 | 23篇 |
2016年 | 43篇 |
2015年 | 85篇 |
2014年 | 91篇 |
2013年 | 103篇 |
2012年 | 154篇 |
2011年 | 121篇 |
2010年 | 114篇 |
2009年 | 84篇 |
2008年 | 88篇 |
2007年 | 94篇 |
2006年 | 92篇 |
2005年 | 72篇 |
2004年 | 74篇 |
2003年 | 57篇 |
2002年 | 60篇 |
2001年 | 10篇 |
2000年 | 3篇 |
1999年 | 8篇 |
1998年 | 9篇 |
1997年 | 2篇 |
1996年 | 7篇 |
1994年 | 3篇 |
1993年 | 1篇 |
1992年 | 2篇 |
1991年 | 1篇 |
1982年 | 1篇 |
1980年 | 1篇 |
1971年 | 1篇 |
排序方式: 共有1527条查询结果,搜索用时 375 毫秒
331.
Inactivation of host Rho GTPases is a widespread strategy employed by bacterial pathogens to manipulate mammalian cellular functions and avoid immune defenses. Some bacterial toxins mimic eukaryotic Rho GTPase-activating proteins (GAPs) to inactivate mammalian GTPases, probably as a result of evolutionary convergence. An intriguing question remains whether eukaryotic pathogens or parasites may use endogenous GAPs as immune-suppressive toxins to target the same key genes as bacterial pathogens. Interestingly, a RhoGAP domain-containing protein, LbGAP, was recently characterized from the parasitoid wasp Leptopilina boulardi, and shown to protect parasitoid eggs from the immune response of Drosophila host larvae. We demonstrate here that LbGAP has structural characteristics of eukaryotic RhoGAPs but that it acts similarly to bacterial RhoGAP toxins in mammals. First, we show by immunocytochemistry that LbGAP enters Drosophila immune cells, plasmatocytes and lamellocytes, and that morphological changes in lamellocytes are correlated with the quantity of LbGAP they contain. Demonstration that LbGAP displays a GAP activity and specifically interacts with the active, GTP-bound form of the two Drosophila Rho GTPases Rac1 and Rac2, both required for successful encapsulation of Leptopilina eggs, was then achieved using biochemical tests, yeast two-hybrid analysis, and GST pull-down assays. In addition, we show that the overall structure of LbGAP is similar to that of eukaryotic RhoGAP domains, and we identify distinct residues involved in its interaction with Rac GTPases. Altogether, these results show that eukaryotic parasites can use endogenous RhoGAPs as virulence factors and that despite their differences in sequence and structure, eukaryotic and bacterial RhoGAP toxins are similarly used to target the same immune pathways in insects and mammals. 相似文献
332.
Transcript abundance profiles reveal larger and more complex responses of grapevine to chilling compared to osmotic and salinity stress 总被引:1,自引:0,他引:1
333.
Gene expression profiling of <Emphasis Type="Italic">Hfe</Emphasis><Superscript>-/-</Superscript>liver and duodenum in mouse strains with differing susceptibilities to iron loading: identification of transcriptional regulatory targets of Hfe and potential hemochromatosis modifiers 下载免费PDF全文
Coppin H Darnaud V Kautz L Meynard D Aubry M Mosser J Martinez M Roth MP 《Genome biology》2007,8(10):R221
334.
335.
336.
337.
Delphine Durieux Bérénice Fassotte Jean‐Louis Deneubourg Yves Brostaux Axel Vandereycken Emilie Joie Eric Haubruge François J. Verheggen 《Insect Science》2015,22(5):670-678
The invasive multicolored Asian ladybeetle, Harmonia axyridis (Pallas) (Coleoptera: Coccinellidae), aggregates inside dwellings during winter to avoid cold weather. This adaptive behavior disturbs homeowners, because of the large numbers of individuals that aggregate, which induces allergic reactions. The migratory flight patterns of this species have been well documented, with individuals preferentially moving toward prominent and high color contrast elements. However, the factors involved in the selection of aggregation sites by this species have yet to be elucidated. Here, we evaluated the influence of (i) the density of individuals and (ii) the type of available shelters on decisions by H. axyridis to settle and aggregate under shelters. A dual choice bioassay conducted in the laboratory demonstrated the presence of mutual attraction to conspecifics. We also found that individuals preferentially settled under red covered shelters compared to transparent shelters, and that the type of shelter outweighed the effect of social interactions among conspecifics. Moreover, this experiment was performed under non‐wintering conditions, providing the first evidence that aggregative behavior in this species can also occur under those specific conditions. 相似文献
338.
339.
340.
Arrighi JF Cartieaux F Brown SC Rodier-Goud M Boursot M Fardoux J Patrel D Gully D Fabre S Chaintreuil C Giraud E 《Molecular plant-microbe interactions : MPMI》2012,25(7):851-861
Research on the nitrogen-fixing symbiosis has been focused, thus far, on two model legumes, Medicago truncatula and Lotus japonicus, which use a sophisticated infection process involving infection thread formation. However, in 25% of the legumes, the bacterial entry occurs more simply in an intercellular fashion. Among them, some Aeschynomene spp. are nodulated by photosynthetic Bradyrhizobium spp. that do not produce Nod factors. This interaction is believed to represent a living testimony of the ancestral state of the rhizobium-legume symbiosis. To decipher the mechanisms of this Nod-independent process, we propose Aeschynomene evenia as a model legume because it presents all the characteristics required for genetic and molecular analysis. It is a short-perennial and autogamous species, with a diploid and relatively small genome (2n=20; 460 Mb/1C). A. evenia 'IRFL6945' is nodulated by the well-characterized photosynthetic Bradyrhizobium sp. strain ORS278 and is efficiently transformed by Agrobacterium rhizogenes. Aeschynomene evenia is genetically homozygous but polymorphic accessions were found. A manual hybridization procedure has been set up, allowing directed crosses. Therefore, it should be relatively straightforward to unravel the molecular determinants of the Nod-independent process in A. evenia. This should shed new light on the evolution of rhizobium-legume symbiosis and could have important agronomic implications. 相似文献