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331.
332.
D. Piesik A. Wenda‐Piesik A. Krasińska D. Wrzesińska K. J. Delaney 《Journal of Applied Entomology》2016,140(4):308-316
We report in this study large induction of volatile organic compounds (VOCs) from a single inflorescence of mossy sorrel (Rumex confertus Willd., Polygonaceae), by herbivory of the weevil (Hypera rumicis L., Coleoptera: Curculionidae). VOCs blend induced by the weevil herbivory included 1 green leaf volatiles (GLVs) ((Z)‐3‐hexen‐1‐yl acetate), five terpenes ((Z)‐β‐ocimene, linalool, geranyl acetate, β‐caryophyllene and (E)‐β‐farnesene), three esters (benzyl acetate, methyl salicylate and methyl anthranilate) and one aromatic heterocyclic organic compound (indole). Uninjured plants produced only detectable amounts of VOCs. A Y‐tube experiment revealed that both females and males of H. rumicis were not attracted to any of tested concentrations (1, 5, 25, 125 ng/min). Also both females and males were significantly repelled by the highest concentrations (25 and 125 ng/min). Additionally, concentration of 5 ng/min proved to be repellent for females of H. rumicis. 相似文献
333.
Ruwanthi N. Gunawardane Preston Fordstrom Derek E. Piper Stephanie Masterman Sophia Siu Dongming Liu Mike Brown Mei Lu Jie Tang Richard Zhang Janet Cheng Andrew Gates David Meininger Joyce Chan Tim Carlson Nigel Walker Margrit Schwarz John Delaney Mingyue Zhou 《The Journal of biological chemistry》2016,291(6):2799-2811
Drug discovery opportunities where loss-of-function alleles of a target gene link to a disease-relevant phenotype often require an agonism approach to up-regulate or re-establish the activity of the target gene. Antibody therapy is increasingly recognized as a favored drug modality due to multiple desirable pharmacological properties. However, agonistic antibodies that enhance the activities of the target enzymes are rarely developed because the discovery of agonistic antibodies remains elusive. Here we report an innovative scheme of discovery and characterization of human antibodies capable of binding to and agonizing a circulating enzyme lecithin cholesterol acyltransferase (LCAT). Utilizing a modified human LCAT protein with enhanced enzymatic activity as an immunogen, we generated fully human monoclonal antibodies using the XenoMouseTM platform. One of the resultant agonistic antibodies, 27C3, binds to and substantially enhances the activity of LCAT from humans and cynomolgus macaques. X-ray crystallographic analysis of the 2.45 Å LCAT-27C3 complex shows that 27C3 binding does not induce notable structural changes in LCAT. A single administration of 27C3 to cynomolgus monkeys led to a rapid increase of plasma LCAT enzymatic activity and a 35% increase of the high density lipoprotein cholesterol that was observed up to 32 days after 27C3 administration. Thus, this novel scheme of immunization in conjunction with high throughput screening may represent an effective strategy for discovering agonistic antibodies against other enzyme targets. 27C3 and other agonistic human anti-human LCAT monoclonal antibodies described herein hold potential for therapeutic development for the treatment of dyslipidemia and cardiovascular disease. 相似文献
334.
Integrative genomics positions MKRN1 as a novel ribonucleoprotein within the embryonic stem cell gene regulatory network
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Paul A Cassar Richard L Carpenedo Payman Samavarchi‐Tehrani Jonathan B Olsen Chang Jun Park Wing Y Chang Zhaoyi Chen Chandarong Choey Sean Delaney Huishan Guo Hongbo Guo R Matthew Tanner Theodore J Perkins Scott A Tenenbaum Andrew Emili Jeffrey L Wrana Derrick Gibbings William L Stanford 《EMBO reports》2015,16(10):1334-1357
In embryonic stem cells (ESCs), gene regulatory networks (GRNs) coordinate gene expression to maintain ESC identity; however, the complete repertoire of factors regulating the ESC state is not fully understood. Our previous temporal microarray analysis of ESC commitment identified the E3 ubiquitin ligase protein Makorin‐1 (MKRN1) as a potential novel component of the ESC GRN. Here, using multilayered systems‐level analyses, we compiled a MKRN1‐centered interactome in undifferentiated ESCs at the proteomic and ribonomic level. Proteomic analyses in undifferentiated ESCs revealed that MKRN1 associates with RNA‐binding proteins, and ensuing RIP‐chip analysis determined that MKRN1 associates with mRNAs encoding functionally related proteins including proteins that function during cellular stress. Subsequent biological validation identified MKRN1 as a novel stress granule‐resident protein, although MKRN1 is not required for stress granule formation, or survival of unstressed ESCs. Thus, our unbiased systems‐level analyses support a role for the E3 ligase MKRN1 as a ribonucleoprotein within the ESC GRN. 相似文献
335.
Hirst M Delaney A Rogers SA Schnerch A Persaud DR O'Connor MD Zeng T Moksa M Fichter K Mah D Go A Morin RD Baross A Zhao Y Khattra J Prabhu AL Pandoh P McDonald H Asano J Dhalla N Ma K Lee S Ally A Chahal N Menzies S Siddiqui A Holt R Jones S Gerhard DS Thomson JA Eaves CJ Marra MA 《Genome biology》2007,8(6):R113-12
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337.
Genome-wide profiles of STAT1 DNA association using chromatin immunoprecipitation and massively parallel sequencing 总被引:2,自引:0,他引:2
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339.
Neeley WL Delaney S Alekseyev YO Jarosz DF Delaney JC Walker GC Essigmann JM 《The Journal of biological chemistry》2007,282(17):12741-12748
Reactive oxygen and nitrogen radicals produced during metabolic processes, such as respiration and inflammation, combine with DNA to form many lesions primarily at guanine sites. Understanding the roles of the polymerases responsible for the processing of these products to mutations could illuminate molecular mechanisms that correlate oxidative stress with cancer. Using M13 viral genomes engineered to contain single DNA lesions and Escherichia coli strains with specific polymerase (pol) knockouts, we show that pol V is required for efficient bypass of structurally diverse, highly mutagenic guanine oxidation products in vivo. We also find that pol IV participates in the bypass of two spiroiminodihydantoin lesions. Furthermore, we report that one lesion, 5-guanidino-4-nitroimidazole, is a substrate for multiple SOS polymerases, whereby pol II is necessary for error-free replication and pol V for error-prone replication past this lesion. The results spotlight a major role for pol V and minor roles for pol II and pol IV in the mechanism of guanine oxidation mutagenesis. 相似文献
340.
Thu-Ha Dinh Kevin P. Delaney Ameena Goga Debra Jackson Carl Lombard Selamawit Woldesenbet Mary Mogashoa Yogan Pillay Nathan Shaffer 《PloS one》2015,10(5)