A Comparative study on the reactions for protein sulphydryl,carboxyl and tyrosine in plant nuclei

V této práci byly aplikovány na rostlinných objektech reakce s β-oxynaftyl-merkurichloridem a p-nitrobromacetofenonem, popsané k pr?kazu bílkovinných SH na materiálu ?ivo?i?ném. V rostlinných meristematických buňkách obě tyto techniky stejně jako DDD¹ a RSR¹ jeví vět?inou intensivněj?í zbarvení jader ne? cytoplasmy. Rovně? po aplikaci reakcí na bílkovinné karboxyly a tyrosin je jádro intensivněji zbarveno ne? cytoplasma. Z toho lze soudit, ?e rozdíly v intensitě zbarvení jádra a cytoplasmy v meristematických buňkách rostlinných technikami k pr?kazu bílkovinných SH jsou zp?sobeny spí? rozdílem v celkové koncentraci bílkovin ne? zv??ením podílu cysteinu v jaderných bílkovinách.     

Influence of Some Ions on the Membrane Potential of Ascaris Muscle

The influence of several ions on the membrane potential of the somatic muscle of Ascaris has been investigated by changing their concentration in the surrounding solution. When [K]_o is increased at the expense of [Na]_o leaving [Cl]_o constant, the membrane potential is first seen to increase. [K]_o higher than 45 mM reduces the membrane potential with a slope of 23 mv for a tenfold change in [K]_o. However, when [K]_o is increased keeping [Na]_o and [Cl]_o low and constant, the line relating the membrane potential with log [K]_o has a slope of almost 50 mv. If [Cl]_o is reduced in the absence of external Na, after the [K]_o is increased to 45 mM, the membrane potential decreases with a slope of 59 mv per tenfold change in [Cl]_o in close agreement with the Nernst equation. If Cl^- is replaced by SO₄ ^2-, a depolarization is produced, while chloride replacement by NO₃ ^-, Br^-, and I^- results in a hyperpolarization of the membrane. Removal of the external Na⁺ ions increases the average membrane potential by 17 mv.     

Calcium dependence of the thrombin-induced increase in endothelial albumin permeability

We examined whether the increase in endothelial albumin permeability induced by alpha-thrombin is dependent on extracellular Ca2+ influx. Permeability of 125I-albumin across confluent monolayers of cultured bovine pulmonary artery endothelial cells was measured before and after the addition of 0.1 microM alpha-thrombin. In the presence of normal extracellular Ca2+ concentration ([Ca2+]o, 1000 microM), alpha-thrombin produced a 175 +/- 10% increase in 125I-albumin permeability. At lower [Ca2+]o (100, 10, 1, or less than 1 microM), alpha-thrombin caused a 140% increase in permeability (P less than 0.005). LaCl3 (1 mM), which competes for Ca2+ entry, blunted 38% of the increase in permeability. Preloading endothelial monolayers with quin2 to buffer cytosolic Ca2+ (Cai2+) produced a dose-dependent inhibition of the increase in 125I-albumin permeability. Preincubation with nifedipine or verapamil was ineffective in reducing the thrombin-induced permeability increase. A 60 mM K+ isosmotic solution did not alter base-line endothelial permeability. alpha-Thrombin increased [Ca2+]i in a dose-dependent manner and the 45Ca2+ influx rate. Extracellular medium containing 60 mM K+ did not increase 45Ca2+ influx, and nifedipine did not block the rise in 45Ca2+ influx caused by alpha-thrombin. Ca2+ flux into endothelial cells induced by alpha-thrombin does not occur through voltage-sensitive channels but may involve receptor-operated channels. In conclusion, the increase in endothelial albumin permeability caused by alpha-thrombin is dependent on Ca2+ influx and intracellular Ca2+ mobilization.     

Distribution of diaphragmatic lymphatic stomata

In seven anesthetized rabbits we measured the size, shape, and density of lymphatic stomata on the peritoneal and pleural sides of the diaphragm. The diaphragm was fixed in situ and processed for scanning electron microscopy. Results are from 2,902 peritoneal and 3,086 pleural fields (each 1,620 microns 2) randomly chosen from the various specimens. Stomata were seen in 9% of the fields examined, and in 30% of the cases they appeared grouped in clusters with 2-14 stomata/field. Stoma density was 250 +/- 242 and 72 +/- 57 (SD) stomata/mm2 on peritoneal and pleural sides, respectively, and it was similar over the muscular and tendinous portion of the two surfaces. The maximum diameter ranged from less than 1 to approximately 30 microns, with an average value of 1.2 +/- 3.1 micron. The ratio of the maximum to the minimum diameter and the surface area averaged 2 +/- 1.4 and 0.7 +/- 2.4 micron 2, respectively. The maximum and minimum diameter and surface area values followed a lognormal frequency distribution, suggesting that stomata geometry is affected by diaphragmatic tension.