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51.
52.
Odor identification: perceptual and semantic dimensions 总被引:8,自引:8,他引:0
53.
S. C. J. Meskers Marcellus Ubbink Gerard W. Canters Harry P. J. M. Dekkers 《Journal of biological inorganic chemistry》1998,3(5):463-469
The pH dependence of the dynamic quenching of the luminescence from Tb(III) and Eu(III) tris(pyridine-2,6-dicarboxylate≡DPA)
chelates by the title proteins is studied. For Tb(DPA)3
3– also the quenching by the Lys 14→Glu and Lys99→Glu mutants of cytochrome c-550 (cytc-550) is investigated. The rate constants for quenching of the electronically excited Λ and Δ enantiomers of the
luminophore by equine cytochrome c show a sharp decrease upon increasing the pH from 7 to 10, which can be described phenomenologically by deprotonation of
a single acidic group with pK
a of 9.2±0.1 for Eu and 9.4±0.1 for Tb. These values are similar to that found for the alkaline transition of the protein.
The alkaline conformer(s) of the protein at pH>10 is found to be a very inefficient quencher of the lanthanide luminescence.
For Tb, but not for Eu, a significant lowering of the degree of enantioselectivity (E
q) in the quenching is found along with a reduction of the quenching rates. For cytc-550, the decrease of the quenching rate
constants with increasing pH is described by pK
a=9.8±0.1 and for the two mutants the same value is obtained. For the cytc-550 proteins the change of the quenching rates does
not correlate with the alkaline transition, for which a pK
a of 11.2 has been reported by other workers. For all proteins, the reduction of the quenching rates at high pH is ascribed
to a reduction of the binding affinity of the excited lanthanide complex to the surface area of the protein near the exposed
heme edge, caused by deprotonation of (presumably) several lysine residues.
Received: 3 April 1998 / Accepted: 15 June 1998 相似文献
54.
Jos M. J. Lamers Dick H. W. Dekkers Karel Bezstarosti Johanna T. A. Meij Han A. A. van Heugten 《Molecular and cellular biochemistry》1992,116(1-2):59-67
In the last decade a great deal of attention was awarded to a signal transduction pathway which is utilized primarily by Ca2+ mobilizing signal molecules and which involves the hydrolysis of a quantitatively minor phospholipid, phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) by a PtdIns-specific phospholipase C (PLC). The evidence for the existence of receptor-mediated GTP binding protein-coupled PLC in myocardium and its possible functions are briefly summarized. The minireview is concentrated on the following aspects: 1) cellular localization and synthesis of polyphospho-PtdIns from PtdIns, 2) desensitization of the 1-adrenergic agonist and endothelin-1 mediated PtdIns responses, 3) oscillatory Ca2+ transients initiated by Ptdlns(4,5)P2 hydrolysis, 4) polyunsaturated fatty acids as constituents of polyphospho-PtdIns and of the protein kinase C activator 1,2-diacylglycerol (DAG), 5) source other than Ptdlns(4,5)P2 contributing to the stimulated DAG, 6) role of the PtdIns pathway in cardiomyocyte growth and gene expression during the hypertrophic response. (Mol Cell Biochem116: 59–67, 1992)Abbreviations Phosphatidylinositol 4,5-bisphosphate
PtdIns(4,5)P2
- Phosphatidylinositol 4-monophosphate
PtdIns(4)P4
- Phosphatidylinositol
PtdIns
- Inositol 1,4,5-triphosphate
Ins(1,4,5)P3
- Inositol 1,3,4,5-tetrakisphosphate
Ins(1,3,4,5)P4
- Inositol 1-monophosphate
Ins(1)P
- Inositol 1,4-bisphosphate
Ins(1,4)P2
- Inositol
Ins
- Inositolphosphates
InsPn
- Guanine 5'-triphosphate
GTP
- GTP binding protein
G-protein
- Phosphatidylinositolspecific phospholipase C
PLC
- Protein kinase C
PKC
- 1,2-Diacylglycerol
DAG
- Monoacylglycerol
MAG
- cytidyldiphoshate-diacylglycerol
CDP-DAG
- Sarcolemma
SL
- Sarcoplasmic reticulum
SR
- Stearic acid
18:0
- Polyunsaturated fatty acids
PUFA
- Arachidonic acid
20:4n-6
- Linoleic acid
18:2n-6
- Eicosapentaenoic acid
20:5n-3
- Docosahexaenoic acid
22:6n-3
- Phosphatidic acid
PtdOH
- Phospholipase D
PLD
- Phosphatidylcholine
PtdChol 相似文献
55.
56.
N. J. Boddicker D. J. Garrick R. R. R. Rowland J. K. Lunney J. M. Reecy J. C. M. Dekkers 《Animal genetics》2014,45(1):48-58
Infectious diseases are costly to the swine industry; porcine reproductive and respiratory syndrome (PRRS) is the most devastating. In earlier work, a quantitative trait locus associated with resistance/susceptibility to PRRS virus was identified on Sus scrofa chromosome 4 using approximately 560 experimentally infected animals from a commercial cross. The favorable genotype was associated with decreased virus load and increased weight gain (WG). The objective here was to validate and further characterize the association of the chromosome 4 region with PRRS resistance using data from two unrelated commercial crossbred populations. The validation populations consisted of two trials each of approximately 200 pigs sourced from different breeding companies that were infected with PRRS virus and followed for 42 days post‐infection. Across all five trials, heritability estimates were 0.39 and 0.34 for viral load (VL; area under the curve of log‐transformed viremia from 0 to 21 days post‐infection) and WG to 42 days post‐infection respectively. Effect estimates of SNP WUR10000125 in the chromosome 4 region were in the same directions and of similar magnitudes in the two new trials as had been observed in the first three trials. Across all five trials, the 1‐Mb region on chromosome 4 explained 15 percent of genetic variance for VL and 11 percent for WG. The effect of the favorable minor allele at SNP WUR10000125 was dominant. Ordered genotypes for SNP WUR10000125 showed that the effect was present irrespective of whether the favorable allele was paternally or maternally inherited. These results demonstrate that selection for host response to PRRS virus infection could reduce the economic impact of PRRS. 相似文献
57.
IC14, an anti-CD14 antibody, inhibits endotoxin-mediated symptoms and inflammatory responses in humans 总被引:14,自引:0,他引:14
Verbon A Dekkers PE ten Hove T Hack CE Pribble JP Turner T Souza S Axtelle T Hoek FJ van Deventer SJ van der Poll T 《Journal of immunology (Baltimore, Md. : 1950)》2001,166(5):3599-3605
CD14 is a receptor for cell wall components of Gram-negative and Gram-positive bacteria that has been implicated in the initiation of the inflammatory response to sepsis. To determine the role of CD14 in LPS-induced effects in humans, 16 healthy subjects received an i.v. injection of LPS (4 ng/kg) preceded (-2 h) by i.v. IC14, a recombinant chimeric mAb against human CD14, at a dose of 1 mg/kg over 1 h, or placebo. In subjects receiving IC14, saturation of CD14 on circulating monocytes and granulocytes was >90% at the time of LPS injection. IC14 attenuated LPS-induced clinical symptoms and strongly inhibited LPS-induced proinflammatory cytokine release, while only delaying the release of the anti-inflammatory cytokines soluble TNF receptor type I and IL-1 receptor antagonist. IC14 also inhibited leukocyte activation, but more modestly reduced endothelial cell activation and the acute phase protein response. The capacity of circulating monocytes and granulocytes to phagocytose Escherichia coli was only marginally reduced after infusion of IC14. These data provide the first proof of principle that blockade of CD14 is associated with reduced LPS responsiveness in humans in vivo. 相似文献
58.
Dekkers CP 《TheScientificWorldJournal》2001,1(Z2):958-967
Emission trading is a new instrument in environmental policy. It is an alien notion in most European countries and it is often viewed with hesitation. The paper discusses the economic, legal, and perhaps more importantly, the cultural aspects to consider when one tries to explore the prospects for trading emissions of NOx and other substances in Europe. Issues to be addressed are the present legal framework in Europe in relation to the national emission ceilings on NOx and other substances on the basis of relevant EU directives and UNECE protocols. The paper will discuss the extent to which the legal framework within the EU imposes constraints on the design of a national emission trading scheme, and what options are available to fit emission trading into that legislative structure. The NOx emission trading programme developed in the Netherlands will be used to demonstrate the various aspects in a European context. 相似文献
59.
60.
James E. Koltes Eric Fritz-Waters Chris J. Eisley Igseo Choi Hua Bao Arun Kommadath Nick V. L. Ser?o Nicholas J. Boddicker Sam M. Abrams Martine Schroyen Hyelee Loyd Chris K. Tuggle Graham S. Plastow Leluo Guan Paul Stothard Joan K. Lunney Peng Liu Susan Carpenter Robert R. R. Rowland Jack C. M. Dekkers James M. Reecy 《BMC genomics》2015,16(1)