全文获取类型
收费全文 | 1847篇 |
免费 | 137篇 |
国内免费 | 2篇 |
专业分类
1986篇 |
出版年
2022年 | 15篇 |
2021年 | 52篇 |
2020年 | 33篇 |
2019年 | 23篇 |
2018年 | 45篇 |
2017年 | 39篇 |
2016年 | 47篇 |
2015年 | 75篇 |
2014年 | 88篇 |
2013年 | 113篇 |
2012年 | 144篇 |
2011年 | 129篇 |
2010年 | 68篇 |
2009年 | 56篇 |
2008年 | 92篇 |
2007年 | 65篇 |
2006年 | 71篇 |
2005年 | 65篇 |
2004年 | 57篇 |
2003年 | 62篇 |
2002年 | 41篇 |
2001年 | 39篇 |
2000年 | 32篇 |
1999年 | 25篇 |
1998年 | 14篇 |
1997年 | 17篇 |
1995年 | 10篇 |
1994年 | 13篇 |
1992年 | 36篇 |
1991年 | 24篇 |
1990年 | 23篇 |
1989年 | 22篇 |
1988年 | 15篇 |
1987年 | 23篇 |
1986年 | 13篇 |
1985年 | 24篇 |
1984年 | 29篇 |
1983年 | 16篇 |
1982年 | 16篇 |
1981年 | 18篇 |
1979年 | 24篇 |
1978年 | 14篇 |
1977年 | 13篇 |
1975年 | 12篇 |
1974年 | 14篇 |
1973年 | 10篇 |
1969年 | 13篇 |
1968年 | 10篇 |
1967年 | 9篇 |
1966年 | 10篇 |
排序方式: 共有1986条查询结果,搜索用时 15 毫秒
11.
In vitro protein synthesis, lysosomal hydrolases activity and peroxidase activity in the anterior pituitary were estimated in adult male rats treated with 50 micrograms of estradiol benzoate (EB) for 1 day or 7 days. Pituitary protein synthesis, protein and RNA content increased after 7 days. A significant increase in total and membrane-bound acid phosphatase was noted after 1 day or 7 days of EB treatment whereas total beta-glucuronidase activity decreased in both 1 and 7 day group. Cathepsin activity increased after 7 days and pituitary peroxidase system did not change by EB treatment. These findings suggest that immediate change in the enzyme milieu may be one of the first reactions by which EB expresses its feedback control. 相似文献
12.
13.
14.
In SDS-PAGE the immune complexes (IC) of kala-azar patient sera showed intense bands at 55 kDa and 20 kDa corresponding to heavy and light chains of immunoglobulins. In immunoblot experiment, kala-azar and normal IC after treatment with patient sera showed multiple bands of which the band at 55 kDa was most prominent in kala-azar IC. It is known that in kala-azar sera antihuman IgG is present, so the heavy band at 55 kDa region may be due to higher amount of IgG and/or other antigen(s) present at that region. Immunoblot experiments of kala-azar IC with anti gp63 also developed a major band at 55 kDa. It suggests that the antigen (55 kDa) and gp63 have common antigenic epitope (s). Normal IC did not react with anti gp63 indicating absence of this antigen in normal IC. Antigenic similarity between the IC antigen (55 kDa) and gp63 indicated that the former antigen may have been processed from gp63. In summary, identification of a parasite antigen (55 kDa) in IC of kala-azar patients sera may be useful in developing a serodiagnostic assay for visceral leishmaniasis. (Mol Cell Biochem130: 11–17, 1994)Abbreviations IC
Immune Complexes
- PEG
Polyethylene Glycol (Mol wt 8000)
- PBS
Phosphate Buffer Saline
- VL
Visceral Leishmaniasis
- AVL
American Visceral Leishmaniasis
- IgG
Immunoglobulin G
- TBS
Tris Buffer Saline
- SDS-PAGE
Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis
- gp63
A leishmanial surface glycoprotein of molecular mass 63,000
- TEMED
N,N,N,N-Tetramethylethylenediamine 相似文献
15.
Transport of bacteria in porous media: II. A model for convective Transport and growth 总被引:1,自引:0,他引:1
A model is presented for the coupled processes of bacterial growth and convective transport of bacteria has been modeled using a fractional flow approach. The various mechanisms of bacteria retention can be incorporated into the model through selection of an appropriate shape of the fractional flow curve. Permeability reduction due to pore plugging by bacteria was simulated using the effective medium theory. In porous media, the rates of transport and growth of bacteria, the generation of metabolic products, and the consumption of nutrients are strongly coupled processes. Consequently, the set of governing conservation equations form a set of coupled, nonlinear partial differential equations that were solved numerically. Reasonably good agreement between the model and experimental data has been obtained indicating that the physical processes incorporated in the model are adequate. The model has been used to predict the in situ transport and growth of bacteria, nutrient consumption, and metabolite production. It can be particularly useful in simulating laboratory experiments and in scaling microbial-enhanced oil recovery or bioremediation processes to the field. (c) 1994 John Wiley & Sons, Inc. 相似文献
16.
A general solution is presented of the problem of specifying all alternative, generally frequency-dependent, (absolute) fitness sets which give rise to the same allele frequency changes and population dynamics as a given fitness set. The one- and two-locus cases are analyzed in detail and the method is then extended to the n-locus case. It is shown that if biological constraints can be used to specify the mean fitness of the population and the relative fitnesses of the heterozygotes, then the allele frequency trajectories determine a unique fitness set. 相似文献
17.
Md. Aktar-Uz-Zaman Md. Ariful Islam Md. Shahin Iqbal Md. Jahangir Alam Debashish Sarkar Bander Albogami Ahmed Gaber Akbar Hossain 《Phyton》2023,92(3):883-899
Ensuring food security for the rapidly increasing population and changing climatic scenarios are requisites for
exploiting the genetic divergence of food crops. A study was undertaken to sort out an early maturing chickpea
variety for fitting easily between rice-rice cropping systems in the Eastern Indo-Gangetic Plain of Bangladesh. The
trial was comprised of eight elite lines of chickpea and executed at various localities in Bangladesh from 2014–
15 to 2017–18. The result explored the chickpea genotype, BARI Chola-11 remained superior to the rest of
the elite genotypes for having a short maturity period (100–106 days), and lesser days to 50% flowering (47–
55 days). The same genotype was recorded to have robust vegetative and reproductive yield attributes including
plant height (49–57 cm), podsplant−1 (37–50), and optimum 100 seed weight (19.5–20.6 g). Owing to better yield
attributes, BARI Chola-11 resulted in the maximum seed yield (1200–1500 kg ha−1
) of chickpea and might be
recommended for general adoption in the region for boosting nutritional security status through improved productivity under changing climate. 相似文献
18.
William R. Trumble Paul V. Viitanen Hemanta K. Sarkar Mohindar S. Poonian H. Ronald Kaback 《Biochemical and biophysical research communications》1984,119(3):860-867
The lac y gene of Escherichia coli which encodes the lac carrier protein has been modified by oligonucleotide-directed, site-specific mutagenesis such that cys148 is converted to a glycine residue. Cells bearing the mutated lac y gene exhibit initial rates of lactose transport that are about 4-fold lower than cells bearing the wild type gene on a recombinant plasmid. Furthermore, transport activity is less sensitive to inactivation by N-ethylmaleimide, and strikingly, galactosyl 1-thio-β-D-galactopyranoside affords no protection against inactivation. The findings suggest that although cys148 is essential for substrate protection against sulfhydryl inactivation, it is not obligatory for lactose:proton symport and that another sulfhydryl group elsewhere within the lac carrier protein may be required for full activity. 相似文献
19.
20.
S. Sarkar 《Genetics》1991,127(2):257-261