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51.
Beneficial effects of n-hexane bark extract of Onosma echioides L. on diabetic peripheral neuropathy
Ambreen Shoaib Badruddeen Rakesh Kumar Dixit Magdah Ganash George Barreto Ghulam Md Ashraf Hefazat Hussain Siddiqui 《Journal of cellular biochemistry》2019,120(10):16524-16532
Onosma echioides Linn (Boraginaceae) is the most frequently used curative herb widely used for kidney obstruction, sciatic pain, and gout. The present study was designed to investigate the therapeutic effects of n-hexane bark extract of O. echioides (OE) L. root in vivo against Streptozotocin-induced diabetic neuropathy in SD rats. For in vivo activity, the experiment was categorized into five different groups (n = 5). Group-I was considered as nondiabetic/normal control (NC) treated with 0.5% carboxymethyl cellulose (CMC), Group II as diabetic control, Group-III, IV, and V served as diabetic treated with OE 50, OE 100, and pregabalin at a dose of 50, 100, and 10 mg/kg body weight, orally, respectively. Body weight, blood glucose, oral glucose tolerance test, behavioral studies (motor coordination test, thermal hyperalgesia, cold allodynia, locomotor activity, oxidative biomarkers (thio barbituric acid reactive substances [TBARS], superoxide dismutase [SOD], glutathione [GSH], and catalase), and histopathology of the sciatic nerve were performed. Treatment with OE showed a dose-dependent increase in neuroprotective activity by improving the myelination and decreasing the axonal swelling of nerve fibers. The verdicts of behavioral activities showed a remarkable effect on animals after the treatment of extract and standard drug pregabalin. In conclusion, our findings supported the traditional application of OE and explored its importance in the management of diabetic neuropathy. Additional clinical experiments may provide novel therapeutic drugs for diabetes and its complications. 相似文献
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A simple method for determination of glutaraldehyde 总被引:1,自引:0,他引:1
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Growth of B95-8 cells and expression of Epstein-Barr virus lytic phase in serum-free medium. 总被引:2,自引:2,他引:0
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Epstein-Barr virus (EBV)-transformed tamarin (Saguinus oedipus) cells (B95-8) were selected for growth in medium with reduced serum and then transferred to serum-free medium which consisted of RPMI 1640 supplemented with insulin, transferrin, and selenium. Serum-free cells in continuous passage for 1 year had a morphology, growth rate, and culture density which approached those of B95-8 cells grown with serum. The cells expressed virus-induced antigens, including the EBV-associated DNA polymerase. Cells exposed to EBV-inducing agents, n-butyric acid and phorbol 12-myristate-13-acetate, produced transforming virus with titers comparable to those of cultures grown with serum. These findings demonstrate that serum is neither required for the growth of B95-8 cells nor necessary for induction or full expression of the EBV lytic phase in these cells. 相似文献
59.
Dipak Kumar Banerjee 《Journal of biosciences》1987,11(1-4):311-319
Radio-labelled amphomycin (3H-amphomycin) forms a complex with dolichylmonophosphate in presence of Ca2+. Complex formation has also been documented with retinylmonophosphate and perhydromonoeneretinylmonophosphate. Analysis of
the space-filling model suggested both fatty acylated aspartic acid residue at the N-terminus of the lipopeptide and phosphate
head group of dolichylmonophosphate are necessary for the complex formation. The binding ability of amphomycin is then utilized
to localize dolichylmonophosphate in the microsomal membrane. Studies with microsomal membranes from hen oviduct suggested
that dolichylmonophosphate is located in the cytoplasmic side of the membrane. 相似文献
60.
S. K. Podder A. Chakraborti K. Vijayalakshmi P. Lalit Kumar Singh 《Journal of biosciences》1987,11(1-4):495-502
To examine how surface Potential controls the reactivity of glycoconjugates at cell surface, the interaction of galactose-sPecific
lectinse.g. peanut agglutinin,Ricinus cummunis agglutinin with liPosomes bearing asialo GM1 were studied in the Presence of varying amount of ganglioside mixture, GMn. The Presence of 5% GMn causes comPlete slowing down of PreciPitin reaction and thereby make carbohydrate moiety of asialo GM1 comPletely inaccessiblei.e. ‘cryPtic’. In contrast the Presence of 1–2% GMn enhances the aPParent rate and amPlitude of the PreciPitin reaction as surface Potential becomes more negative. The relevance
of the findings has been discussed in relation to the exPression and involvement of the cell-surface sialic acid residues
during develoPment and differentiation. 相似文献