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61.
Logan J. Pallin Nick M. Kellar Debbie Steel Natalia Botero-Acosta C. Scott Baker Jack A. Conroy Daniel P. Costa Chris M. Johnson David W. Johnston Ross C. Nichols Doug P. Nowacek Andrew J. Read Oksana Savenko Oscar M. Schofield Sharon E. Stammerjohn Deborah K. Steinberg Ari S. Friedlaender 《Global Change Biology》2023,29(8):2108-2121
The krill surplus hypothesis of unlimited prey resources available for Antarctic predators due to commercial whaling in the 20th century has remained largely untested since the 1970s. Rapid warming of the Western Antarctic Peninsula (WAP) over the past 50 years has resulted in decreased seasonal ice cover and a reduction of krill. The latter is being exacerbated by a commercial krill fishery in the region. Despite this, humpback whale populations have increased but may be at a threshold for growth based on these human-induced changes. Understanding how climate-mediated variation in prey availability influences humpback whale population dynamics is critical for focused management and conservation actions. Using an 8-year dataset (2013–2020), we show that inter-annual humpback whale pregnancy rates, as determined from skin-blubber biopsy samples (n = 616), are positively correlated with krill availability and fluctuations in ice cover in the previous year. Pregnancy rates showed significant inter-annual variability, between 29% and 86%. Our results indicate that krill availability is in fact limiting and affecting reproductive rates, in contrast to the krill surplus hypothesis. This suggests that this population of humpback whales may be at a threshold for population growth due to prey limitations. As a result, continued warming and increased fishing along the WAP, which continue to reduce krill stocks, will likely impact this humpback whale population and other krill predators in the region. Humpback whales are sentinel species of ecosystem health, and changes in pregnancy rates can provide quantifiable signals of the impact of environmental change at the population level. Our findings must be considered paramount in developing new and more restrictive conservation and management plans for the Antarctic marine ecosystem and minimizing the negative impacts of human activities in the region. 相似文献
62.
Maura Francolini Marialuisa Lavitrano Carla Lora Lamia Deborah French Luigi Frati Franco Cotelli Corrado Spadafora 《Molecular reproduction and development》1993,34(2):133-139
Mature sperm cells have the spontaneous capacity to take up exogenous DNA. Such DNA specifically interacts with the subacrosomal segment of the sperm head corresponding to the nuclear area. Part of the sperm-bound foreign DNA is further internalized into nuclei. Using end-labelled plasmid DNA we have found that 15–22% of the total sperm bound DNA is associated with nuclei as determined on isolated nuclei. On the basis of autoradiographic analysis, nuclear permeability to exogenous DNA seems to be a wide phenomenon involving the majority of the sperm nuclei. In fact, the foreign DNA, incubated with sperm cells for different lengths of time, is found in 45% (10 min) to 65% (2 hr) of the sperm nuclei. Ultrastructural autoradiography on thin sections of mammalian spermatozoa, preincubated with end-labelled plasmid DNA, shows that the exogenous DNA is internalized into the nucleus. This conclusion is further supported by ultrastructural autoradiographic analysis on thin sections of nuclei isolated from spermatozoa preincubated with end-labelled DNA. © 1993 Wiley-Liss, Inc. 相似文献
63.
Deborah M. Gordon 《Evolution & development》2023,25(6):430-438
Collective behavior operates without central control, using local interactions among participants to adjust to changing conditions. Many natural systems operate collectively, and by specifying what objectives are met by the system, the idea of agency helps to describe how collective behavior is embedded in the conditions it deals with. Ant colonies function collectively, and the enormous diversity of more than 15K species of ants, in different habitats, provides opportunities to look for general ecological patterns in how collective behavior operates. The foraging behavior of harvester ants in the desert regulates activity to manage water loss, while the trail networks of turtle ants in the canopy tropical forest respond to rapidly changing resources and vegetation. These examples illustrate some broad correspondences in natural systems between the dynamics of collective behavior and the dynamics of the surroundings. To outline how interactions among participants, acting in relation with changing surroundings, achieve collective outcomes, I focus on three aspects of collective behavior: the rate at which interactions adjust to conditions, the feedback regime that stimulates and inhibits activity, and the modularity of the network of interactions. To characterize the dynamics of the surroundings, I consider gradients in stability, energy flow, and the distribution of resources and demands. I then propose some hypotheses that link how collective behavior operates with changing environments. 相似文献
64.
65.
Jeffery B. Press James J. McNally Pauline J. Sanfilippo Michael F. Addo Deborah Loughney Edward Giardino Laurence B. Katz Robert Falotico Barbara J. Haertlein 《Bioorganic & medicinal chemistry》1993,1(6):423-435
The syntheses and antihypertensive activity of the thieno[3,4-b]pyran and thieno[2,3-b]pyran isosteres of the potassium channel opener (PCO) RWJ 26629 (± 2a) are reported. While the unsubstituted thiophene derivatives were active at 20 mg/kg, introduction of a strong electron withdrawing group in the 2-position of the thieno[3,2-b] series increased potency. Similar substitution on the thieno[3,4-b] series significantly lowered potency. Compounds 26 and 30 are approximately 5-fold more potent than the prototypic PCO cromakalim (± 1). 相似文献
66.
Marissa L. Miller Deborah M. Brown Tadeusz A. Wysocki 《Biotechnology and bioengineering》2023,120(2):562-571
Influenza A viruses (IAV) have been the cause of several influenza pandemics in history and are a significant threat for the next global pandemic. Hospitalized influenza patients often have excess interferon production and a dysregulated immune response to the IAV infection. Obtaining a better understanding of the mechanisms of IAV infection that induce these harmful effects would help drug developers and health professionals create more effective treatments for IAV infection and improve patient outcomes. IAV stimulates viral sensors and receptors expressed by alveolar epithelial cells, like RIG-I and toll-like receptor 3 (TLR3). These two pathways coordinate with one another to induce expression of type III interferons to combat the infection. Presented here is a queuing theory-based model of these pathways that was designed to analyze the timing and amount of interferons produced in response to IAV single stranded RNA and double-stranded RNA detection. The model accurately represents biological data showing the necessary coordination of the RIG-I and TLR3 pathways for effective interferon production. This model can serve as the framework for future studies of IAV infection and identify new targets for potential treatments. 相似文献
67.
Stephanie S. Mayer Deborah Charlesworth 《Evolution; international journal of organic evolution》1992,46(1):207-215
Dioecy is unusually common in the Hawaiian Islands, yet little is known about the evolutionary biology of this breeding system. A native shrub, Wikstroemia, has an unusually diverse array of breeding systems: two forms of dioecy, cryptic and morphological dioecy, as well as hermaphroditism (perfect flowers). The existence of two forms of dioecy is significant for three reasons: 1) the presence of cryptic unisexuals that are functionally unisexual, but retain the appearance of hermaphroditism in both sexes, is strong evidence for the ancestral status of hermaphroditism; 2) the production of nonfunctional pollen, by female cryptic unisexuals, is a new instance of a phenomenon which has previously been reported for a few other species; 3) the two forms of dioecy are morphological markers which are useful in hybridization studies for tracing the genetic basis of their inheritance. Crosses were made between cryptically unisexual individuals (C), between morphologically unisexual individuals (M), and between the two types of unisexuality. The offspring of crosses between individuals with the same sex type usually resulted in offspring with that sex type, but most of the progeny of between-sex type crosses were, unexpectedly, perfect-flowered hermaphrodites. These results show that genetic control of sex determination is not homologous in all populations, suggesting that dioecy has evolved at least twice in Hawaiian Wikstroemia. The genetic data further suggest that males are the heterozygous sex. 相似文献
68.
The following nucleoside diphosphate monosaccharides (sugar nucleotides) were identified by HPLC from Pterocladia capillacea Born and Thur.: ADP-glucose, UDP-glucose, UDP-d -galactose, and GDP-glucose + mannose. GDP-l -galactose was not identified due to the lack of a standard. Several extraction methods were evaluated for their efficacy. A freeze/ thaw (liquid N2) step fallowed by formic acid (1 M) extraction, reduced pressure evaporation, and solubilization in water was the preferred method. Differences in media nitrate that resulted in different tissue-N levels (1.8, 2.3, and 3.5% dry wt) and agar yields (34, 31, and 28% dry wt, respectively) also resulted in a marked difference in UDP-d -galactose and ADP-glucose tissue levels (decrease with increasing tissue-N) while the levels of the other sugar nucleotide agar precursors remained unchanged. Activities of UDP-glucose, GDP-glucose, and GDP-mannose pyrophosphorylases, and UDP-D-glucose-4-epimerase were detected in cell-free extracts using unlabeled and 14C-labeled substrates. This study-strongly supports the proposition that the d -galactose component of agar is synthesized via G-1-P → UDP-glucose→ UDP-d -galactose and that, the l -galactoae component is produced via mannose-1-P → GDP-mannose → GDP-l -galactose. 相似文献
69.
Deborah M. Briercheck Timothy J. Allison John P. Richardson Jeffery F. Ellena Todd C. Wood Gordon S. Rule 《Journal of biomolecular NMR》1996,8(4):429-444
Summary Protein fragments containing the RNA-binding domain of Escherichia coli rho protein have been over-expressed in E. coli. NMR spectra of the fragment containing residues 1–116 of rho protein (Rho116) show that a region of this protein is unfolded in solution. Addition of (dC)10 to this fragment stabilizes the folded form of the protein. The fragment comprising residues 1–130 of rho protein (Rho130) is found to be stably folded, both in the absence and presence of nucleic acid. NMR studies of the complex of Rho 130 with RNA and DNA oligonucleotides indicate that the binding-site size, affinity, and specificity of Rho 130 are similar to those of intact rho protein; therefore, Rho 130 is a suitable model of the RNA-binding domain of rho protein. NMR line widths as well as titration experiments of Rho130 complexed with oligonucleotides of various lengths suggest that Rho130 forms oligomers in the presence of longer oligonucleotides. 1H, 15N and 13C resonance assignments were facilitated by the utilization of two pulse sequences, CN-NOESY and CCH-TOCSY. The secondary structure of unliganded Rho130 has been determined by NMR techniques, and it is clear that the RNA-binding domain of rho is more structurally similar to the cold shock domain than to the RNA recognition motif.Abbreviations Rho116, Rho130
protein containing the first 116 (130) residues of rho
- CSD
cold shock domain
- RRM
RNA recognition motif
- RBD
RNA-binding domain
- IPTG
isopropyl -D-thiogalactopyranoside
- EDTA
ethylenediaminetetraacetic acid
- NOE
nuclear Overhauser enhancement 相似文献
70.