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101.
Ramesh N. Patel Mark Liu Amit Banerjee Laszlo J. Szarka 《Applied microbiology and biotechnology》1992,37(2):180-183
Summary A key chiral intermediate lactol(3)[3aS (3a,4,7,7a)]-hexahydro-4,7-epoxy-isobenzofuran-1 (3H)-one was prepared for the total synthesis of a new thromboxane antagonist. The stereoselective hydrolysis of (exo,exo)-7-oxabicyclo[2.2.1]heptane-2,3-dimethanol, diacetate ester (1) to the corresponding chiral monoacetate ester (2) was carried out with lipases, among which Amano P-30 lipase from Pseudomonas sp. was most effective since it gave the desired enantiomer of monoacetate ester. A yield of 75 mol% and optical purity of >99% was obtained when the reaction was conducted in a biphasic system with 10% toluene at 5 g/l of the substrate. Lipase P-30 was immobilized on Accurel polypropylene (PP) and the immobilized enzyme was reused (five cycles) without loss of enzyme activity, productivity or optical purity. The reaction process was scaled-up to 80 1 (400 g substrate) and monoacetate (2) was isolated in 80 mol% yield with 99.3% optical purity as determined by chiral HPLC and nuclear magnetic resonance (NMR) analysis. A gas chromatography of 99.5% and specific rotation, []D of -7.6° was obtained. The chiral monoacetate ester (2) was oxidized to its corresponding aldehyde and subsequently hydrolyzed to give lactol (3). 相似文献
102.
103.
104.
Bagchi D Roy S Patel V He G Khanna S Ojha N Phillips C Ghosh S Bagchi M Sen CK 《Molecular and cellular biochemistry》2006,281(1-2):197-209
Edible berry extracts rich in anthocyanins possess a broad spectrum of therapeutic, pharmacologic and anti-carcinogenic properties.
Six berry extracts (wild blueberry, bilberry, cranberry, elderberry, raspberry seeds and strawberry), singly and in combination,
were studied in our laboratories for antioxidant efficacy, cytotoxic potential, cellular uptake and anti-angiogenic properties.
Combinations of edible berry extracts were evaluated to develop a synergistic formula, OptiBerry, which exhibited high oxygen
radical absorbance capacity (ORAC) value, low cytotoxicity and superior anti-angiogenic properties compared to the other combinations
tested. The current study sought to determine the broad spectrum safety and antioxidant potential of OptiBerry in vivo. Acute oral LD50 of OptiBerry was greater than 5 g/kg in rats. Acute dermal LD50 of OptiBerry was greater than 2 g/kg. No changes in the body weight or adverse effects were observed following necropsy.
Primary skin and eye irritation studies were conducted in New Zealand albino rabbits. OptiBerry was classified as slightly
irritating to the skin (primary skin irritation index 0.3) and minimally irritating to the eye (maximum mean total score 6.0).
The antioxidant potential of OptiBerry was investigated in rats and mice by assessing GSH redox status in tissues as well
as by a unique state-of-the-art electron paramagnetic resonance (EPR) imaging of whole-body redox status. A clinically relevant
hyperbaric oxygen (HBO) exposure system (2 atm, 2 h) was employed to study the antioxidant properties of OptiBerry. OptiBerry
feeding (8 weeks) significantly prevented HBO-induced GSH oxidation in the lung and liver of vitamin E-deficient Sprague Dawley
rats. Furthermore, OptiBerry-fed mice, when exposed to HBO, demonstrated significant protection in whole-body HBO-induced
oxidation compared to the unfed controls by EPR imaging. Taken together, these results indicate that OptiBerry is reasonably
safe and possess antioxidant properties. 相似文献
105.
106.
A new 3,7-dioxabicyclo[3,3,O]octane lignan, named justisolin, and a new lignan O-glucoside, named simplexoside, were isolated from the whole plant of Justicia simplex D. Don. (Acanthaceae), collected at fruiting. The structure of the free lignan was established as 2e-(3,4-methylenedioxy-6-hydroxy)-phenyl-6e- Piperonyl-3,7-dioxabicyclo[3,3,0]octane (1) and that of the glucoside as 2e-(3-methoxy-4-O-β-d- glucopyranosyl)-phenyl-6e-piperonyl-3,7-dioxabicyclo[3,3,0]octane (2) on the basis of chemical transformation and spectral evidence. The biological functions of these and related lignans are appraised. 相似文献
107.
Aditi Banerjee Aloke K. Ghoshal 《International biodeterioration & biodegradation》2011,65(7):1052-1060
Phenol degradation by Bacillus cereus AKG1 MTCC9817 and AKG2 MTCC 9818 was investigated and degradation kinetics are reported for the free and Ca-alginate gel-immobilized systems. The optimal pH for maximum phenol degradation by immobilized AKG1 and AKG2 was found to be 6.7 and 6.9, respectively, while 3% alginate was optimum for both the strains. The degradation of phenol by free as well as immobilized cells was comparable at lower concentrations of phenol (100–1000 mg l−1). However, the degradation efficiency of the immobilized strains was higher than that of the free strains at higher phenol concentrations (1500–2000 mg l−1), indicating the improved tolerance of the immobilized cells toward phenol toxicity. More than 50% of 2000 mg l−1 phenol was degraded by immobilized AKG1 and AKG2 within 26 and 36 days, respectively. Degradation kinetics of phenol by free and immobilized cells are well represented by the Haldane and Yano model. 相似文献
108.
Annapurna Kumari Paramita Mahapatra Vijay Kumar Garlapati Rintu Banerjee 《Biotechnology for biofuels》2009,2(1):1-7
Background
Transesterification of Jatropha oil was carried out in t-butanol solvent using immobilized lipase from Enterobacter aerogenes. The presence of t-butanol significantly reduced the negative effects caused by both methanol and glycerol. The effects of various reaction parameters on transesterification of Jatropha oil were studied. 相似文献109.
Manju Shri Arti Rai Pankaj Kumar Verma Prashant Misra Sonali Dubey Smita Kumar Sikha Verma Neelam Gautam Rudra Deo Tripathi Prabodh Kumar Trivedi Debasis Chakrabarty 《Protoplasma》2013,250(2):631-636
Agrobacterium-mediated transformation of indica rice varieties has been quite difficult as these are recalcitrant to in vitro responses. In the present study, we established a high-efficiency Agrobacterium tumefaciens-mediated transformation system of rice (Oryza sativa L. ssp. indica) cv. IR-64, Lalat, and IET-4786. Agrobacterium strain EHA-101 harboring binary vector pIG121-Hm, containing a gene encoding for β-glucuronidase (GUS) and hygromycin resistance, was used in the transformation experiments. Manipulation of different concentrations of acetosyringone, days of co-culture period, bacterial suspension of different optical densities (ODs), and the concentrations of l-cysteine in liquid followed by solid co-culture medium was done for establishing the protocol. Among the different co-culture periods, 5 days of co-culture with bacterial cells (OD600 nm?=?0.5–0.8) promoted the highest frequency of transformation (83.04 %) in medium containing l-cysteine (400 mg l?1). Putative transformed plants were analyzed for the presence of a transgene through genomic PCR and GUS histochemical analyses. Our results also suggest that different cultural conditions and the addition of l-cysteine in the co-culture medium improve the Agrobacterium-mediated transformation frequencies from an average of 12.82 % to 33.33 % in different indica rice cultivars. 相似文献