全文获取类型
收费全文 | 4509篇 |
免费 | 631篇 |
国内免费 | 6篇 |
出版年
2022年 | 38篇 |
2021年 | 60篇 |
2020年 | 45篇 |
2019年 | 45篇 |
2018年 | 50篇 |
2017年 | 55篇 |
2016年 | 74篇 |
2015年 | 141篇 |
2014年 | 160篇 |
2013年 | 228篇 |
2012年 | 226篇 |
2011年 | 268篇 |
2010年 | 168篇 |
2009年 | 142篇 |
2008年 | 241篇 |
2007年 | 245篇 |
2006年 | 217篇 |
2005年 | 219篇 |
2004年 | 210篇 |
2003年 | 246篇 |
2002年 | 212篇 |
2001年 | 109篇 |
2000年 | 118篇 |
1999年 | 118篇 |
1998年 | 78篇 |
1997年 | 65篇 |
1996年 | 59篇 |
1995年 | 61篇 |
1994年 | 51篇 |
1993年 | 45篇 |
1992年 | 68篇 |
1991年 | 62篇 |
1990年 | 70篇 |
1989年 | 63篇 |
1988年 | 54篇 |
1987年 | 56篇 |
1986年 | 50篇 |
1985年 | 66篇 |
1984年 | 59篇 |
1983年 | 47篇 |
1982年 | 47篇 |
1981年 | 40篇 |
1980年 | 51篇 |
1979年 | 33篇 |
1978年 | 31篇 |
1977年 | 33篇 |
1976年 | 23篇 |
1974年 | 39篇 |
1971年 | 27篇 |
1970年 | 19篇 |
排序方式: 共有5146条查询结果,搜索用时 156 毫秒
71.
A soluble 2,3-oxidosqualene sterol cyclase 总被引:5,自引:0,他引:5
P D Dean P R Ortiz de Montellano K Bloch E J Corey 《The Journal of biological chemistry》1967,242(12):3014-3015
72.
Metabolism of [1-14C]glyoxylate, [1-14C]-glycollate, [1-14C]glycine and [2-14C]-glycine by homogenates of kidney and liver tissue from hyperoxaluric and control subjects 总被引:1,自引:1,他引:0
下载免费PDF全文
![点击此处可从《The Biochemical journal》网站下载免费的PDF全文](/ch/ext_images/free.gif)
1. The metabolism of [1-(14)C]glyoxylate to carbon dioxide, glycine, oxalate, serine, formate and glycollate was investigated in hyperoxaluric and control subjects' kidney and liver tissue in vitro. 2. Only glycine and carbon dioxide became significantly labelled with (14)C, and this was less in the hyperoxaluric patients' kidney tissue than in the control tissue. 3. Liver did not show this difference. 4. The metabolism of [1-(14)C]glycollate was also studied in the liver tissue; glyoxylate formation was demonstrated and the formation of (14)CO(2) from this substrate was likewise unimpaired in the hyperoxaluric patients' liver tissue in these experiments. 5. Glycine was not metabolized by human kidney, liver or blood cells under the conditions used. 6. These observations show that glyoxylate metabolism by the kidney is impaired in primary hyperoxaluria. 相似文献
73.
The wrestler's ear 总被引:2,自引:0,他引:2
J C Kelleher J G Sullivan G J Baibak R K Dean 《Plastic and reconstructive surgery》1967,40(6):540-546
74.
1. 26-Hydroxycholesterol was obtained by reducing the methyl ester of (±)-3β-hydroxycholest-5-en-26-oic acid, which was synthesized from 25-oxonorcholesterol. 2. Methods for preparing 7α-hydroxycholesterol and 7-dehydrocholesterol were modified to allow the micro-scale preparation of these [14C]sterols from [26-14C]-cholesterol. 3. 26-Hydroxycholesterol was oxidized more readily than 7α-hydroxycholesterol, 7-dehydrocholesterol or cholesterol by mitochondrial preparations from livers of mice, rats, guinea pigs, common toads (Bufo vulgaris) and Caiman crocodylus. 4. (±)-3β-Hydroxy[26-14C]cholest-5-en-26-oic acid was oxidized very rapidly to 14CO2 by mouse and guinea-pig mitochondria without evident discrimination between the two optical isomers. 5. An enzyme system that oxidizes 26-hydroxycholesterol to 3β-hydroxycholest-5-en-26-oic acid was identified in the soluble extract of rat-liver mitochondria. This enzyme could use NADP in place of NAD but was not identical with liver alcohol dehydrogenase (EC 1.1.1.1). 6. [26-14C]Cholesteryl 3β-sulphate was not oxidized by fortified mouse-liver preparations that oxidized [26-14C]cholesterol to 14CO2. 相似文献
75.
76.
Geoffrey Dean 《BMJ (Clinical research ed.)》1959,2(5156):852-857
77.
78.
79.
Sequences 5'' to translation start regulate expression of petunia rbcS genes. 总被引:5,自引:4,他引:1
下载免费PDF全文
![点击此处可从《The Plant cell》网站下载免费的PDF全文](/ch/ext_images/free.gif)
The promoter sequences that contribute to quantitative differences in expression of the petunia genes (rbcS) encoding the small subunit of ribulose bisphosphate carboxylase have been characterized. The promoter regions of the two most abundantly expressed petunia rbcS genes, SSU301 and SSU611, show sequence similarity not present in other rbcS genes. We investigated the significance of these and other sequences by adding specific regions from the SSU301 promoter (the most strongly expressed gene) to equivalent regions in the SSU911 promoter (the least strongly expressed gene) and assaying the expression of the fusions in transgenic tobacco plants. In this way, we characterized an SSU301 promoter region (either from -285 to -178 or -291 to -204) which, when added to SSU911, in either orientation, increased SSU911 expression 25-fold. This increase was equivalent to that caused by addition of the entire SSU301 5'-flanking region. Replacement of SSU911 promoter sequences between -198 and the start codon with sequences from the equivalent region of SSU301 did not increase SSU911 expression significantly. The -291 to -204 SSU301 promoter fragment contributes significantly to quantitative differences in expression between the petunia rbcS genes. 相似文献
80.
From studies based on batch culture, it has been postulated that the expression of the virulence-associated proteins of Yersinia spp. is controlled by temperature and Ca2+ , such that these proteins are synthesized only at the higher temperature (37°C) and calcium-scarce conditions of the intracellular environment. It was found, however, that in Yersinia enterocolitica one of these proteins (140 kDa) is not synthesized at submaximal growth rates under any of the relevant conditions, and that another of the implicated proteins (34 kDa), is synthesized even at 28°C during nutrient-limited growth. Thus, temperature and Ca2+ influence the synthesis of these proteins differently under growth conditions that better approximate the natural environments than do batch cultures. 相似文献