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301.
Tritium concentration ratios of less than unity (0·19) were determined for adult crickets which were at equilibrium for that particular instar with a tritium tagged food source. This phenomenon is interpreted as meaning that simple diffusion-equilibrium assumptions are not adequate for describing tritium movement in food chains when true equilibrium conditions are not met. Additional factors, such as rates of tritium assimilation and turnover for tissue-bound and body-water fractions, food habits, and duration of developmental stages must be considered along with environmental tritium concentrations in determining food chain transfers of this radionuclide. 相似文献
302.
F J Cassels D L Jarboe R H Reid A Lees C D Deal 《Journal of industrial microbiology & biotechnology》1997,19(1):66-70
Enterotoxigenic Escherichia coli (ETEC) cause diarrhea in infants and in travelers to developing countries. The bacteria utilize colonization factors (CF)
for adherence to intestinal epithelia, then release toxins causing diarrhea. CF are strong immunogens as well as protective
antigens. While 20 ETEC CF have been described in the literature, 11 CF are prominent enough to be considered for vaccine
targeting. Of this group, six of the members fall into the CFA/I family of CF. Geysen pin (peptide) linear epitope analysis
demonstrated that three regions containing linear epitopes exist in CFA/I, and that both B- and T-cell linear epitopes of
CFA/I were concentrated at the N-terminus of the protein. We have determined N-terminal sequence of the CFA/I family members
not previously sequenced. Comparison of the protein sequence of the six members of the family showed a strong homology up
to residue 36. A peptide of 36 amino acids representing a consensus of the six sequences was synthesized and used to immunize
animals. The antibody induced to the peptide was reactive to the peptide as well as cross-reactive to each member of the
CFA/I family in Western blots. In addition, this antibody agglutinated three of the six members of the CFA/I family when
added to whole cells expressing the native CF. We are currently evaluating different carriers and conjugation methods to
maximize production of high titer, agglutinating antibody. It is hoped that this and related research will result in an
effective and inexpensive cross-reactive and cross-protective ETEC vaccine.
Received 04 October 1996/ Accepted in revised form 14 April 1997 相似文献