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171.
Correlates of protection from rotavirus infection are controversial. We compared the roles of B and T lymphocytes in protective immunity induced either by intranasally administered nonreplicating viruslike particles or inactivated virus or by orally administered murine rotavirus. We found that protection induced by nonreplicating vaccines requires CD4+ T cells and CD40/CD40L. In contrast, T cells were not required for short-term protective immunity induced by infection, but both T-cell-dependent and -independent mechanisms contributed to long-term maintenance of protection. Our findings indicate that more than one marker of protective immunity exists and that these markers depend on the vaccine that is administered. 相似文献
172.
M. O’Callaghan E. M. Gerard N. W. Waipara S. D. Young T. R. Glare P. J. Barrell A. J. Conner 《Plant and Soil》2005,266(1-2):47-56
Antimicrobial peptide magainin II, isolated from the skin of the African clawed toad, has shown activity in vitro against a range of micro-organisms. Transgenic potato lines expressing a synthetic magainin gene show improved resistance
to the bacterial plant pathogen, Erwinia carotovora. Culturable bacterial and fungal communities associated with magainin-producing potato plants were compared with those communities
from the non-transgenic parental control and with another potato cultivar. Total numbers of aerobic bacteria recovered from
the leaves of the magainin-producing line, its non-transgenic parent line and an unrelated cultivar did not differ significantly.
There were no detectable differences in the numbers of Gram-positive and Gram-negative bacteria, pseudomonad populations or
fungi recovered from foliage from the three plant lines. Bacterial populations recovered from the roots of a magainin-expressing
plant line did not differ significantly from populations recovered from the unmodified parental line. Tubers from the magainin-expressing
transgenic potatoes, however, had significantly lower total numbers of bacteria than tubers produced by unmodified plants.
In vitro testing of rhizosphere isolates against magainin analogues found that bacterial isolates varied in their susceptibility to
the peptides. There were no significant differences in the total numbers of fungi and yeasts recovered from the various plant
lines, with one exception: higher numbers of fungi were recovered from roots of magainin-expressing plants than the unmodified
control plants. 相似文献
173.
174.
Production of Transgenic Soybean Plants with Two Anti-Fungal Protein Genes Via Agrobacterium and Particle Bombardment 总被引:1,自引:0,他引:1
H.Y. Li Y.M. Zhu Q. Chen R.L. Conner X.D. Ding J. Li B.B. Zhang 《Biologia Plantarum》2004,48(3):367-374
Utilizing either Agrobacterium-mediated transformation or particle bombardment we obtained transgenic soybean [Glycine max (L.) Merr.] plants expressing the chitinase gene (chi) and the barley ribosome-inactivating protein gene (rip). Six regenerated plants were grown to maturity and set seed. The identification of transgenic soybean plants that co-integrated the two anti-fungal protein genes was determined by polymerase chain reaction (PCR) and Southern blot analysis. Protein detection from the soybean leaves demonstrated the expression of the chitinase (CHI) and the ribosome-inactivating protein (RIP) in the six R0 transformants. Soybean cotyledonary nodes were transformed using the bivalent plant expression vector pBRC containing chi and rip both driven by the CaMV 35S double promoter. Following vacuum (0.06 MPa) infiltration treatment of the tissue for 5 min, Agrobacterium was co-cultivated with the cotyledonary nodes for 3 d on MSB medium (MS salts and B5 vitamins) (pH 5.2), the transformation frequency reached a maximum of 1.33 %. The chi and rip genes were present in all the transgenic plants. Co-bombardment of immature cotyledons with plasmids pBchE (encoding chi) and pARIP (encoding rip) resulted in a maximum transformation frequency of 0.52 % with a 50 % co-integration rate. Our results demonstrate efficient co-transformation of multiple genes in soybean. 相似文献
175.
Meghan A Duffy Chad E Brassil Spencer R Hall Alan J Tessier Carla E Cáceres Jeffrey K Conner 《BMC evolutionary biology》2008,8(1):80
Background
A mismatch has emerged between models and data of host-parasite evolution. Theory readily predicts that parasites can promote host diversity through mechanisms such as disruptive selection. Yet, despite these predictions, empirical evidence for parasite-mediated increases in host diversity remains surprisingly scant. 相似文献176.
Not all weeds are created equal: A database approach uncovers differences in the sexual system of native and introduced weeds
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Megan L. Van Etten Jeffrey K. Conner Shu‐Mei Chang Regina S. Baucom 《Ecology and evolution》2017,7(8):2636-2642
Weedy species provide excellent opportunities to examine the process of successful colonization of novel environments. Despite the influence of the sexual system on a variety of processes from reproduction to genetic structure, how the sexual system of species influences weediness has received only limited consideration. We examined the hypothesis that weedy plants have an increased likelihood of being self‐compatible compared with nonweedy plants; this hypothesis is derived from Baker's law, which states that species that can reproduce uniparentally are more likely to successfully establish in a new habitat where mates are lacking. We combined a database of the weed (weedy/nonweedy) and introduction status (introduced/native) of plant species found in the USA with a database of plant sexual systems and determined whether native and introduced weeds varied in their sexual systems compared with native and introduced nonweeds. We found that introduced weeds are overrepresented by species with both male and female functions present within a single flower (hermaphrodites) whereas weeds native to the USA are overrepresented by species with male and female flowers present on a single plant (monoecious species). Overall, our results show that Baker's law is supported at the level of the sexual system, thus providing further evidence that uniparental reproduction is an important component of being either a native or introduced weed. 相似文献
177.
Hardwood management and restoration of longleaf pine ecosystems may affect raccoon daytime resting sites
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Fire‐maintained Pinus palustris (longleaf pine) ecosystems are species rich and considered a top conservation priority in the southeastern United States. Ground‐nesting species such as Gopherus polyphemus (gopher tortoise) and Colinus virginianus (northern bobwhite) thrive in longleaf ecosystems. However, the generalist carnivore Procyon lotor (raccoon) is a significant predator of these endemic ground nesters. In forested ecosystems, raccoons prefer hardwood‐dominated habitats. Removal of hardwood trees, which is a common longleaf pine ecosystem restoration tool, affects habitat use of this predator. We examined 269 daytime resting sites (DRS) associated with 31 radio‐collared adult raccoons (18 M, 13 F) during 2014–2015 on a longleaf pine‐dominated site in southwestern Georgia. We developed 26 a priori models using an information theoretic approach to evaluate factors affecting use of DRS by raccoons. The top two models (ΔAIC < 2) had combined model weights of 0.75 and contained tree diameter, tree type, presence of nearby hardwood, and distances to pine, hardwood, mixed forest, and agriculture as predictors. However, the only informative variables were tree type and tree diameter. Raccoons used DRS in all available forest types, but were less likely to use pine trees (n = 7) relative to hardwoods (n = 247), and there was a positive relationship with tree diameter. Females used smaller trees farther from agriculture and primary roads, and were closer to wetlands than those used by males. Hardwood removal from within longleaf pine ecosystem affects habitat use of this predator, specifically DRS. 相似文献
178.
Christine E. Conner Rodney W. Kelly Robert Hume 《Histochemistry and cell biology》2001,116(4):313-319
Specialisation of the respiratory portion of human fetal lung commences around 20-24 weeks gestation. In contrast, human fetal lung in vitro has the capacity to self-differentiate from 12 weeks gestation when grown in media devoid of growth factors or hormones, suggesting activation of autocrine or paracrine factors in vitro, or removal of the fetus from in utero inhibitory mechanisms. Prostaglandins play a key role during in vitro human fetal lung development and are synthesised by prostaglandin H synthase-1 (PGHS-1) and inactivated by 15-hydroxyprostaglandin dehydrogenase (PGDH) with formation of inactive 13,14-dihydro-15-keto-prostaglandins. We have used quantitative immunohistochemistry to determine expression and localisation of PGHS-1, PGDH, PGE2 and 13,14-dihydro-15-keto-PGE2 (PGEM) in human fetal lung with in situ hybridisation to localise PGHS-1 and PGDH mRNA. For the catabolic enzyme PGDH, amounts of mRNA, protein and enzyme product PGEM are increased within epithelium of distal as compared to more proximal airways. For PGHS-1, comparable amounts of mRNA, protein and enzyme product PGE2 are found in proximal and distal lung epithelium. Catabolism by PGDH is a sensitive mechanism for regulating bioavailability of prostaglandins and we propose that active catabolism of prostaglandins within human fetal lung epithelium is an inhibitory mechanism retarding epithelial differentiation in utero. 相似文献
179.
180.
Andrew P. Castagno Till J. W. Wagner Mattias R. Cape Conner W. Lester Elizabeth Bailey Catharina Alves-de-Souza Robert A. York Alyson H. Fleming 《Global Change Biology》2023,29(17):5087-5098
Phytoplankton primary production in the Arctic Ocean has been increasing over the last two decades. In 2019, a record spring bloom occurred in Fram Strait, characterized by a peak in chlorophyll that was reached weeks earlier than in other years and was larger than any previously recorded May bloom. Here, we consider the conditions that led to this event and examine drivers of spring phytoplankton blooms in Fram Strait using in situ, remote sensing, and data assimilation methods. From samples collected during the May 2019 bloom, we observe a direct relationship between sea ice meltwater in the upper water column and chlorophyll a pigment concentrations. We place the 2019 spring dynamics in context of the past 20 years, a period marked by rapid change in climatic conditions. Our findings suggest that increased advection of sea ice into the region and warmer surface temperatures led to a rise in meltwater input and stronger near-surface stratification. Over this time period, we identify large-scale spatial correlations in Fram Strait between increased chlorophyll a concentrations and increased freshwater flux from sea ice melt. 相似文献