A high-throughput assay shows that DNase-I binds actin monomers and polymers with similar affinity

Previous conflicting reports suggest that DNase-I binds F-actin with either equal or drastically different K(D) values compared to G-actin. We developed a high-throughput DNase-I inhibition assay to determine the K(D) of DNase-I for F-actin. We confirmed that phalloidin-stabilized F-actin is protected from depolymerization by DNase-I and that the critical concentration at the pointed end of phalloidin-F-actin is 45.5+/-13.9 nM. We found that DNase-I inhibition by actin follows ultrasensitive mechanics. Using varying lengths of gelsolin-capped phalloidin-F-actin, we concluded that the affinities of DNase-I for G- and the pointed end subunits of F-actin are almost indistinguishable, such that DNase-I may not distinguish between G- and F-actin conformations.     

Beyond isohydricity: The role of environmental variability in determining plant drought responses

Despite the appeal of the iso/anisohydric framework for classifying plant drought responses, recent studies have shown that such classifications can be strongly affected by a plant's environment. Here, we present measured in situ drought responses to demonstrate that apparent isohydricity can be conflated with environmental conditions that vary over space and time. In particular, we (a) use data from an oak species (Quercus douglasii) during the 2012–2015 extreme drought in California to demonstrate how temporal and spatial variability in the environment can influence plant water potential dynamics, masking the role of traits; (b) explain how these environmental variations might arise from climatic, topographic, and edaphic variability; (c) illustrate, through a “common garden” thought experiment, how existing trait‐based or response‐based isohydricity metrics can be confounded by these environmental variations, leading to Type‐1 (false positive) and Type‐2 (false negative) errors; and (d) advocate for the use of model‐based approaches for formulating alternate classification schemes. Building on recent insights from greenhouse and vineyard studies, we offer additional evidence across multiple field sites to demonstrate the importance of spatial and temporal drivers of plants' apparent isohydricity. This evidence challenges the use of isohydricity indices, per se, to characterize plant water relations at the global scale.     

Fine scale distribution for a population of New Zealand's only endemic dolphin (Cephalorhynchus hectori) shows long‐term stability of coastal hotspots

Marine mammal populations often have “hotspots” of distribution. Understanding what drives these is important for understanding relationships with habitat and evaluating exposure to threats. Few studies investigate the stability of hotspots, yet this information is vital in assessing their importance. In this study, over 9,000 sightings made during systematic surveys over 29 yr are used to establish the existence, locations, and temporal dynamics of hotspots for Hector's dolphins at Banks Peninsula, New Zealand. Sightings were divided into four seasons and three time periods to assess temporal trends in habitat use. Kernel density analysis was performed on sightings, weighted by survey effort. Density values at hotspots and reference areas were modeled according to season and time period using linear mixed models. Fifty percent of weighted sightings (n = 4,513) occurred within 21% of the study area. Hotspots had significantly higher densities during summer and these high‐density areas have remained consistent over time. Such consistency implies importance of these areas to the dolphins' ecology. This information adds to our knowledge of how this endangered species uses its habitat, suggests candidate areas for protection from threats, and provides a baseline for assessing habitat related impacts on Hector's dolphins at Banks Peninsula.© 2018 Society for Marine Mammalogy     

Why the Long Face? Kangaroos and Wallabies Follow the Same ‘Rule’ of Cranial Evolutionary Allometry (CREA) as Placentals

Evolutionary Biology - Among closely related species, larger mammals tend to have a longer face and proportionally smaller braincase. This putative ‘rule’ in mammalian macroevolution...     

LRRK2 pathobiology in Parkinson's disease

Mutations in the catalytic Roc‐COR and kinase domains of leucine‐rich repeat kinase 2 (LRRK2) are a common cause of familial Parkinson's disease (PD). LRRK2 mutations cause PD with age‐related penetrance and clinical features identical to late‐onset sporadic PD. Biochemical studies support an increase in LRRK2 kinase activity and a decrease in GTPase activity for kinase domain and Roc‐COR mutations, respectively. Strong evidence exists that LRRK2 toxicity is kinase dependent leading to extensive efforts to identify selective and brain‐permeable LRRK2 kinase inhibitors for clinical development. Cell and animal models of PD indicate that LRRK2 mutations affect vesicular trafficking, autophagy, protein synthesis, and cytoskeletal function. Although some of these biological functions are affected consistently by most disease‐linked mutations, others are not and it remains currently unclear how mutations that produce variable effects on LRRK2 biochemistry and function all commonly result in the degeneration and death of dopamine neurons. LRRK2 is typically present in Lewy bodies and its toxicity in mammalian models appears to be dependent on the presence of α‐synuclein, which is elevated in human iPS‐derived dopamine neurons from patients harboring LRRK2 mutations. Here, we summarize biochemical and functional studies of LRRK2 and its mutations and focus on aberrant vesicular trafficking and protein synthesis as two leading mechanisms underlying LRRK2‐linked disease.

     

The fur of mammals in exposed environments; do crypsis and thermal needs necessarily conflict? The polar bear and marsupial koala compared

The furs of mammals have varied and complex functions. Other than for thermoregulation, fur is involved in physical protection, sensory input, waterproofing and colouration, the latter being important for crypsis or camouflage. Some of these diverse functions potentially conflict. We have investigated how variation in cryptic colouration and thermal features may interact in the coats of mammals and influence potential heat inflows from solar radiation, much of which is outside the visible spectral range. The coats of the polar bear (Ursus maritimus) and the marsupial koala (Phascolarctus cinereus) have insulative similarities but, while they feature cryptic colouration, they are of contrasting colour, i.e. whitish and dark grey. The reflectance of solar radiation by coats was measured across the full solar spectrum using a spectroradiometer. The modulation of incident solar radiation and resultant heat flows in these coats were determined at a range of wind speeds by mounting them on a heat flux transducer/temperature-controlled plate apparatus in a wind tunnel. A lamp with a spectral distribution of radiation similar to the solar spectrum was used as a proxy for the sun. Crypsis by colour matching was apparent within the visible spectrum for the two species, U. maritimus being matched against snow and P. cinereus against Eucalyptus forest foliage. While reflectances across the full solar spectrum differed markedly, that of U. maritimus being 66 % as opposed to 10 % for P. cinereus, the heat influxes from solar radiation reaching the skin were similar. For both coats at low wind speed (1 m s^?1), 19 % of incident solar radiation impacted as heat at the skin surface; at higher wind speed (10 m s^?1) this decreased to approximately 10 %. Ursus maritimus and P. cinereus have high and comparable levels of fur insulation and although the patterns of reflectance and depths of penetrance of solar radiation differ for the coats, the considerable insulation limited the radiant heat reaching the skin. These data suggest that generally, if mammal coats have high insulation then heat flow from solar radiation into an animal is much restricted and the impact of coat colour is negligible. However, comparisons with published data from other species suggest that as fur insulation decreases, colour increasingly influences the heat inflow associated with solar radiation.