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91.
Hermans IF Silk JD Gileadi U Masri SH Shepherd D Farrand KJ Salio M Cerundolo V 《Journal of immunology (Baltimore, Md. : 1950)》2007,178(5):2721-2729
The quality of signals received by dendritic cells (DC) in response to pathogens influences the nature of the adaptive response. We show that pathogen-derived signals to DC mediated via TLRs can be modulated by activated invariant NKT (iNKT) cells. DC maturation induced in vivo with any one of a variety of TLR ligands was greatly improved through simultaneous administration of the iNKT cell ligand alpha-galactosylceramide. DC isolated from animals treated simultaneously with TLR and iNKT cell ligands were potent stimulators of naive T cells in vitro compared with DC from animals treated with the ligands individually. Injection of protein Ags with both stimuli resulted in significantly improved T cell and Ab responses to coadministered protein Ags over TLR stimulation alone. Ag-specific CD8(+) T cell responses induced in the presence of the TLR4 ligand monophosphoryl lipid A and alpha-galactosylceramide showed faster proliferation kinetics, and increased effector function, than those induced with either ligand alone. Human DC exposed to TLR ligands and activated iNKT cells in vitro had enhanced expression of maturation markers, suggesting that a cooperative action of TLR ligands and iNKT cells on DC function is a generalizable phenomenon across species. These studies highlight the potential for manipulating the interactions between TLR ligands and iNKT cell activation in the design of effective vaccine adjuvants. 相似文献
92.
Identification of key tissue type for antler regeneration through pedicle periosteum deletion 总被引:5,自引:0,他引:5
Epimorphic regeneration is the “holy grail” of regenerative medicine. Research aimed at investigating the various models of
epimorphic regeneration is essential if a fundamental understanding of the factors underpinning this process are to be established.
Deer antlers are the only mammalian appendages that are subject to an annual cycle of epimorphic regeneration. In our previous
studies, we have reported that histogenesis of antler regeneration relies on cells resident within the pedicle periosteum
(PP). The present study elaborates this finding by means of functional studies involving the deletion of PP. Four yearling
and four 2-year-old stags were selected for total PP deletion or partial PP deletion experiments. Of the animals in the total
PP deletion group, one showed no signs of antler regeneration throughout the antler growth season. Two showed substantial
and one showed marginal delays in antler regeneration (at 34, 20 and 7 days, respectively) compared with the corresponding
sham-operated sides. Histological investigation revealed that the delayed antlers were derived from regenerated PP. Unexpectedly,
the regenerative capacity of the antler from the total periosteum-deleted pedicles depended on antler length at surgery. Of
the four deer that had partial PP deletion, two regenerated antlers exclusively from the left-over PP on the pedicle shafts
in the absence of participation from the pedicle bone proper. The combined results from the PP deletion experiments convincingly
demonstrate that the cells of the PP are responsible for antler regeneration.
The authors thank the New Zealand Foundation of Research, Science and Technology and Deer Industry New Zealand for funding
their research. 相似文献
93.
The extended Moran effect and large-scale synchronous fluctuations in the size of great tit and blue tit populations 总被引:1,自引:0,他引:1
Saether BE Engen S Grøtan V Fiedler W Matthysen E Visser ME Wright J Møller AP Adriaensen F van Balen H Balmer D Mainwaring MC McCleery RH Pampus M Winkel W 《The Journal of animal ecology》2007,76(2):315-325
1. Synchronous fluctuations of geographically separated populations are in general explained by the Moran effect, i.e. a common influence on the local population dynamics of environmental variables that are correlated in space. Empirical support for such a Moran effect has been difficult to provide, mainly due to problems separating out effects of local population dynamics, demographic stochasticity and dispersal that also influence the spatial scaling of population processes. Here we generalize the Moran effect by decomposing the spatial autocorrelation function for fluctuations in the size of great tit Parus major and blue tit Cyanistes caeruleus populations into components due to spatial correlations in the environmental noise, local differences in the strength of density regulation and the effects of demographic stochasticity. 2. Differences between localities in the strength of density dependence and nonlinearity in the density regulation had a small effect on population synchrony, whereas demographic stochasticity reduced the effects of the spatial correlation in environmental noise on the spatial correlations in population size by 21.7% and 23.3% in the great tit and blue tit, respectively. 3. Different environmental variables, such as beech mast and climate, induce a common environmental forcing on the dynamics of central European great and blue tit populations. This generates synchronous fluctuations in the size of populations located several hundred kilometres apart. 4. Although these environmental variables were autocorrelated over large areas, their contribution to the spatial synchrony in the population fluctuations differed, dependent on the spatial scaling of their effects on the local population dynamics. We also demonstrate that this effect can lead to the paradoxical result that a common environmental variable can induce spatial desynchronization of the population fluctuations. 5. This demonstrates that a proper understanding of the ecological consequences of environmental changes, especially those that occur simultaneously over large areas, will require information about the spatial scaling of their effects on local population dynamics. 相似文献
94.
Rayner K Chen YX Hibbert B White D Miller H Postel EH O'Brien ER 《American journal of physiology. Regulatory, integrative and comparative physiology》2007,292(2):R743-R750
While estrogen receptor (ER) profile plays an important role in response to estrogens, receptor coregulators act as critical determinants of signaling. Although the clinical effects of ovarian hormones on various normal and pathological processes are an active area of research, the exact signaling effects on, for example, the vessel wall, are incompletely understood. Hence, we sought to discover proteins that associate with ERbeta, the isoform that shows upregulated mRNA expression after arterial injury. Using a yeast two-hybrid screen we identified NM23-H2, a multifaceted metastasis suppressor candidate protein, as an ERbeta-associated protein. Although NM23-H2 was immunodetected in arteries from young subjects (27 +/- 6 yr, 14 men and 6 women) with benign intimal hyperplasia, expression was diminished in fatty streaks/atheromas and altogether absent in advanced atherosclerotic lesions. Both nm23-H2 mRNA and protein were expressed by vascular cells in vitro. Treatment with 17beta-estradiol and an ERbeta-selective agonist, diarylpropionitrile, increased protein expression of NM23-H2; an effect that was not seen with an ERalpha-selective agonist, propylpyrazole-triol. Estrogen also prompted nuclear localization of NM23-H2 protein in human coronary smooth muscle cells (SMCs). An in vitro mimic of inflammation decreased the expression of NM23-H2 in SMCs, which was restored on addition of estrogen and dependent on the estrogen receptor. In summary, we report the novel association of NM23-H2 with ERbeta and show for the first time its expression in vascular cells and demonstrate regulation of its expression and localization by estrogen. In that the abundance of NM23-H2 diminishes with both the advancement of atherosclerosis and inflammation, this ERbeta-associated protein may play an important role in mediating the vasculoprotective effects of estrogens. 相似文献
95.
Complex interactions with females and rival males limit the evolution of sperm offence and defence 总被引:1,自引:0,他引:1
Bjork A Starmer WT Higginson DM Rhodes CJ Pitnick S 《Proceedings. Biological sciences / The Royal Society》2007,274(1619):1779-1788
Postcopulatory sexual selection favours males which are strong offensive and defensive sperm competitors. As a means of identifying component traits comprising each strategy, we used an experimental evolution approach. Separate populations of Drosophila melanogaster were selected for enhanced sperm offence and defence. Despite using a large outbred population and evidence of substantive genetic variation for each strategy, neither trait responded to selection in the two replicates of this experiment. Recent work with fixed chromosome lines of D. melanogaster suggests that complex genotypic interactions between females and competing males contribute to the maintenance of this variation. To determine whether such interactions could explain our lack of response to selection on sperm offence and defence, we quantified sperm precedence across multiple sperm competition bouts using an outbred D. melanogaster population exhibiting continuous genetic variation. Both offensive and defensive sperm competitive abilities were found to be significantly repeatable only across matings involving ejaculates of the same pair of males competing within the same female. These repeatabilities decreased when the rival male stayed the same but the female changed, and they disappeared when both the rival male and the female changed. Our results are discussed with a focus on the complex nature of sperm precedence and the maintenance of genetic variation in ejaculate characteristics. 相似文献
96.
Dawn B. Neill 《Human nature (Hawthorne, N.Y.)》2007,18(3):209-224
Health research has shown that overweight and obesity in children and adults are becoming significant public health problems
in the developing world. Evidence suggests that this phenomenon is more marked in urban than rural areas and may be associated
with modernization. However, the underlying reasons for this nutrition transition remain unclear. Dietary shifts, often in
conjunction with income and time constraints in urban environments, may entail a greater reliance on more convenient sugar
and fat-dense food. Also, the necessity of labor-intensive agricultural work to meet rural subsistence needs is supplanted
in urban environments by sedentary work. This paper extends the application of human behavioral ecology theory into the realm
of international development and policy by applying Kaplan’s embodied capital theory to explore differences in food habits
and nutritional status of Indo-Fijian children within the context of urbanization. Urban high-embodied-capital women demonstrate
higher rates of wage-earning employment than urban low-embodied-capital or rural women. Findings indicate that urban high-embodied-capital
households spend significantly more on food purchases, purchase a greater proportion of processed foods, and have children
with higher body mass indexes (BMI) than do urban low-embodied-capital or rural households. This suggests that urban high-embodied-capital
mothers, who tend to be employed, may be making trade-offs between income and food choices.
相似文献
Dawn B. NeillEmail: |
97.
Anderson DE 《Journal of strength and conditioning research / National Strength & Conditioning Association》2007,21(1):169-172
The purpose of this study was to examine the reliability of an air displacement plethysmography device (BOD POD) over trials performed on 3 different days. Subjects consisted of 24 healthy adults (8 men, 16 women), ages 18-38 years, with body weights 46.8-93.6 kg, body mass indexes of 19.1-30.1 kg x m(-2), and percentage body fats (BF) of 7.9-43.1%. Two estimates of BF were performed on 3 days. Paired t-tests revealed no significant within-day differences in body volume (BV), thoracic gas volume (V(TG)), body density (BD), and BF. Correlations between the two V(TG) measures on a day were r = 0.86 for day 1, r = 0.93 for day 2, and r = 0.96 for day 3. BF estimates within a day had high correlations of r = 0.98. Significant differences were found between days for measures of BV, V(TG), BD, and BF. These results indicate a high reliability for within-day estimates of BF and significant differences in between-day estimates of BF using air displacement plethysmography. Reliability of BF may be increased by requiring subjects to practice the procedure for V(TG) measurement. 相似文献
98.
99.
The ex situ population of maned wolves is not self‐sustaining due to poor reproduction, caused primarily by parental incompetence. Studies have shown that environmental enrichment can promote natural parental behaviors in zoo animals. The objective of this study was to determine the effects of environmental enrichment on behavioral and physiological responses of maned wolves. During an 8‐week experimental period, daily behavior observations and fecal sample collection were conducted on four adult wolves (2.2) individually housed in environments without enrichment. After 2 weeks, the wolves were chronologically provided with 2‐week intervals of hiding dead mice around the exhibit, no enrichment, and introduction of boomer balls. Responses of the wolves to enrichment were assessed based on activity levels and exploratory rates, as well as the level of corticoid metabolites in fecal samples collected daily throughout the study period. Providing wolves with environmental enrichment significantly increased exploratory behaviors (P<0.05), especially when mice were hidden in the enclosure. Fecal corticoid concentrations were increased during periods of enrichment in males (P<0.05), but not in females. Overall, there were no correlations between behavioral responses to enrichment and fecal corticoid levels. Behavioral results suggest that environmental enrichment elicits positive effects on the behavior of captive maned wolves. There is evidence suggesting that providing animals with ability to forage for food is a more effective enrichment strategy than introducing objects. There is need for a longer term study to determine the impact of environmental enrichment in this species. Zoo Biol 26:331–343, 2007. © 2007 Wiley‐Liss, Inc. 相似文献
100.