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31.
Rui Cheng Xiaoman Zheng Yingmei Wang Xing Ma Xin Liu Wenjun Xu Mengyun Wang Yuanpeng Gao Xupeng Xing Chuan Zhou Hongzheng Sun Zekun Guo Fusheng Quan Jun Liu Song Hua Yongsheng Wang Yong Zhang Xu Liu 《中国科学:生命科学英文版》2022,65(11):2257-2268
Animal cloning can be achieved by somatic cell nuclear transfer(SCNT), but the resulting live birth rate is relatively low. We previously improved the efficiency of bovine SCNT by exogenous melatonin treatment or by overexpression of lysine-specific demethylase 4D(KDM4D) and 4E(KDM4E). In this study, we revealed abundant alternative splicing(AS) transitions during fertilization and embryonic genome activation, and demonstrated abnormal AS in bovine SCNT embryos compared with in vitro fertilized ... 相似文献
32.
Yingxin Ma Guobin Mao Weishan Yang Guoqiang Wu Guoqiang Li Xiaoying Li Xin Lin Junnan Lu Shijun Zhao Wei Zhao Junbiao Dai Xian-En Zhang 《中国科学:生命科学英文版》2022,65(9):1894-1897
<正>Dear Editor,Severe acute respiratory syndrome coronavirus 2(SARSCoV-2) was identified as the pathogen causing the coronavirus disease(COVID-19), which sometimes resulted in fatal pneumonia(Hu et al., 2021). SARS-CoV-2 is a biosafety level 3(BSL-3) pathogen, and the requirement for high containment conditions is a bottleneck for basic research on viral biology. 相似文献
33.
不同农业土地利用方式和管理对土壤有机碳的影响
——以北京市延庆盆地为例
总被引:7,自引:1,他引:6
——以北京市延庆盆地为例
总被引:7,自引:1,他引:6
自20世纪80年代以来,我国农业土地利用方式和农田管理发生了巨大变化,由此引起的土壤有机碳(SOC)含量、密度及其垂直分布发生了相应的变化,研究不同土地利用方式和管理对土壤有机碳的影响对于探讨农田生态系统的固碳作用具有重要的意义.以北京市延怀盆地为典型研究地区,选择6种农业土地利用和管理模式,共计42块样地,在1m深土体内分层采集197个土壤样品.研究结果表明:(1) 不同农业土地利用和管理方式对SOC含量的影响主要发生在0~25 cm土层中,剖面中SOC含量自上向下明显降低.(2) 通过对6种土地利用和管理方式下土壤SOC含量进行比较,结果发现果园和高投入的玉米地土壤在0~100 cm土层中SOC含量均较高,变化范围分别为4.16~10.00 g kg^-1和4.73~9.31 g kg^-1; 菜地土壤在0~40 cm土层中SOC含量较高,变化范围为6.42~9.67 g kg^-1;大豆地、中、低投入玉米地土壤在0~100 cm土层中SOC含量较低,变化范围分别为3.27~7.73 g kg^-1、3.14~8.33 g kg^-1和1.83~7.67 g kg^-1. (3) 不同农业土地利用方式对SOC密度影响的趋势与对SOC含量影响的趋势基本一致,在0~100 cm土壤中,SOC密度的顺序为果园>菜地>高投入玉米地>中投入玉米地>大豆地>低投入玉米地,变化范围为4.15~8.22 kg m^-2. 相似文献
34.
Chang Ge Huakang Sheng Xin Chen Xiaolin Shen Xinxiao Sun Yajun Yan Jia Wang Qipeng Yuan 《Biotechnology journal》2020,15(6)
Quorum sensing (QS) is a ubiquitous cell–cell communication mechanism in microbes that coordinates population‐level cell behaviors, such as biofilm production, virulence, swarming motility, and bacterial persistence. Efforts to engineer QS systems to take part in metabolic network regulation represent a promising strategy for synthetic biology and pathway engineering. Recently, design, construction, and implementation of QS circuits for programmed control of bacterial phenotypes and metabolic pathways have gained much attention, but have not been reviewed recently. In this article, the architectural organizations and genetic contributions of the naturally occurring QS components to understand the mechanisms are summarized. Then, the most recent progress in application of QS toolkits to develop synthetic networks for novel cell behaviors creation and metabolic pathway engineering is highlighted. The current challenges in large‐scale application of these QS circuits in synthetic biology and metabolic engineering fields are discussed and future perspectives for further engineering efforts are provided. 相似文献
35.
Liu X Follmer D Zweier JR Huang X Hemann C Liu K Druhan LJ Zweier JL 《Biochemistry》2012,51(25):5072-5082
The endogenous vasodilator nitric oxide (NO) is metabolized in tissues in an O(2)-dependent manner. This regulates NO levels in the vascular wall; however, the underlying molecular basis of this O(2)-dependent NO consumption remains unclear. While cytoglobin (Cygb) was discovered a decade ago, its physiological function remains uncertain. Cygb is expressed in the vascular wall and can consume NO in an O(2)-dependent manner. Therefore, we characterize the process of the O(2)-dependent consumption of NO by Cygb in the presence of the cellular reductants and reducing systems ascorbate (Asc) and cytochrome P(450) reductase (CPR), measure rate constants of Cygb reduction by Asc and CPR, and propose a reaction mechanism and derive a related kinetic model for this O(2)-dependent NO consumption involving Cygb(Fe(3+)) as the main intermediate reduced back to ferrous Cygb by cellular reductants. This kinetic model expresses the relationship between the rate of O(2)-dependent consumption of NO by Cygb and rate constants of the molecular reactions involved. The predicted rate of O(2)-dependent consumption of NO by Cygb is consistent with experimental results supporting the validity of the kinetic model. Simulations based on this kinetic model suggest that the high efficiency of Cygb in regulating the NO consumption rate is due to the rapid reduction of Cygb by cellular reductants, which greatly increases the rate of consumption of NO at higher O(2) concentrations, and binding of NO to Cygb, which reduces the rate of consumption of NO at lower O(2) concentrations. Thus, the coexistence of Cygb with efficient reductants in tissues allows Cygb to function as an O(2)-dependent regulator of NO decay. 相似文献
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39.
低钾胁迫对不同基因型水稻苗期根系生长和内源激素含量的影响 总被引:3,自引:0,他引:3
以2个耐低钾基因型水稻N18、N19和2个低钾敏感基因型水稻N27、N28为材料,采用溶液培养试验,研究低钾胁迫对其苗期根系生长和内源激素含量的影响。结果表明,低钾胁迫下,水稻根长、地上部干重和根干重均降低,但N18和N19显著高于N27和N28。低钾胁迫使4个基因型水稻的根冠比增大,而各基因型之间差异不显著。低钾胁迫下,水稻根中IAA、GA1和ZR含量均减少,ABA含量增加;N18、N19根中IAA、GA1和ZR含量都高于N27、N28。此外,低钾胁迫使水稻根中IAA/ABA、ZR/ABA、GA1/ABA值降低,但N18、N19的上述比值高于N27、N28。 相似文献
40.
rmlB and rmlC genes are essential for growth of mycobacteria 总被引:4,自引:0,他引:4