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91.
Background
Organisms are capable of developing different phenotypes by altering the genes they express. This phenotypic plasticity provides a means for species to respond effectively to environmental conditions. One of the most dramatic examples of phenotypic plasticity occurs in the highly social hymenopteran insects (ants, social bees, and social wasps), where distinct castes and sexes all arise from the same genes. To elucidate how variation in patterns of gene expression affects phenotypic variation, we conducted a study to simultaneously address the influence of developmental stage, sex, and caste on patterns of gene expression in Vespula wasps. Furthermore, we compared the patterns found in this species to those found in other taxa in order to investigate how variation in gene expression leads to phenotypic evolution. 相似文献92.
AB Zarafi AM Emechebe AD Akpa O Alabi 《Archives Of Phytopathology And Plant Protection》2013,46(4):261-268
Pearl millet downy mildew (DM) incidence, severity and yield losses of two pearl millet varieties (local and improved) due to the disease were determined in the field. Significant differences in the disease incidence and severity were recorded in the plots sown with metalaxyl-treated seeds and those sown with non-treated seeds, indicating the efficacy of the fungicide on the fungus. Yield losses due to non-treatment of seeds with metalaxyl was 40.88 and 45.39% in a local variety and 43.00 and 18.60% in an improved variety in the 2000 and 2001 cropping seasons respectively. Significant differences between plots sown with metalaxyl-treated and those sown with non-treated seeds were obtained for other yield components such as 1000-grains weight, panicle length and weight. 相似文献
93.
Sequence variation in nuclear ribosomal small subunit,internal transcribed spacer and large subunit regions of Rhizophagus irregularis and Gigaspora margarita is high and isolate‐dependent 下载免费PDF全文
Odile Thiéry Martti Vasar Teele Jairus John Davison Christophe Roux Paula‐Ann Kivistik Andres Metspalu Lili Milani Ülle Saks Mari Moora Martin Zobel Maarja Öpik 《Molecular ecology》2016,25(12):2816-2832
Arbuscular mycorrhizal (AM) fungi are known to exhibit high intra‐organism genetic variation. However, information about intra‐ vs. interspecific variation among the genes commonly used in diversity surveys is limited. Here, the nuclear small subunit (SSU) rRNA gene, internal transcribed spacer (ITS) region and large subunit (LSU) rRNA gene portions were sequenced from 3 to 5 individual spores from each of two isolates of Rhizophagus irregularis and Gigaspora margarita. A total of 1482 Sanger sequences (0.5 Mb) from 239 clones were obtained, spanning ~4370 bp of the ribosomal operon when concatenated. Intrasporal and intra‐isolate sequence variation was high for all three regions even though variant numbers were not exhausted by sequencing 12–40 clones per isolate. Intra‐isolate nucleotide variation levels followed the expected order of ITS > LSU > SSU, but the values were strongly dependent on isolate identity. Single nucleotide polymorphism (SNP) densities over 4 SNP/kb in the ribosomal operon were detected in all four isolates. Automated operational taxonomic unit picking within the sequence set of known identity overestimated species richness with almost all cut‐off levels, markers and isolates. Average intraspecific sequence similarity values were 99%, 96% and 94% for amplicons in SSU, LSU and ITS, respectively. The suitability of the central part of the SSU as a marker for AM fungal community surveys was further supported by its level of nucleotide variation, which is similar to that of the ITS region; its alignability across the entire phylum; its appropriate length for next‐generation sequencing; and its ease of amplification in single‐step PCR. 相似文献
94.
UPTAKE AND RELEASE OF TAURINE FROM RAT BRAIN SLICES 总被引:5,自引:8,他引:5
Abstract— Rapid efflux of [35 S]taurine from rat brain slices was observed on electrical stimulation. Slower release resulted when the Ca2+ content of the perfusion medium was replaced with Mg2+ . Uptake of [35 S]taurine into rat cortical slices was unaffected by GABA, glutamic acid, glycine and leucine but was inhibited by alanine, ouabain, KCN and 2,4-dinitrophenol. Of a number of analogues of taurine, 2-aminoethylsulphinic acid was the most potent in inhibiting the uptake of [35 S]taurine. The rate of uptake was found to be decreased by lowering the incubation temperature. The possibility that taurine may be a neurotransmitter is discussed. 相似文献
95.
96.
Fabbro T Davison AC Steinger T 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2007,115(7):933-944
Many quantitative genetic statistics are functions of variance components, for which a large number of replicates is needed
for precise estimates and reliable measures of uncertainty, on which sound interpretation depends. Moreover, in large experiments
the deaths of some individuals can occur, so methods for analysing such data need to be robust to missing values. We show
how confidence intervals for narrow-sense heritability can be calculated in a nested full-sib/half-sib breeding design (males
crossed with several females) in the presence of missing values. Simulations indicate that the method provides accurate results,
and that estimator uncertainty is lowest for sampling designs with many males relative to the number of females per male,
and with more females per male than progenies per female. Missing data generally had little influence on estimator accuracy,
thus suggesting that the overall number of observations should be increased even if this results in unbalanced data. We also
suggest the use of parametrically simulated data for prior investigation of the accuracy of planned experiments. Together
with the proposed confidence intervals an informed decision on the optimal sampling design is possible, which allows efficient
allocation of resources. 相似文献
97.
Kovacheva VP Mellott TJ Davison JM Wagner N Lopez-Coviella I Schnitzler AC Blusztajn JK 《The Journal of biological chemistry》2007,282(43):31777-31788
During gestation there is a high demand for the essential nutrient choline. Adult rats supplemented with choline during embryonic days (E) 11-17 have improved memory performance and do not exhibit age-related memory decline, whereas prenatally choline-deficient animals have memory deficits. Choline, via betaine, provides methyl groups for the production of S-adenosylmethionine, a substrate of DNA methyltransferases (DNMTs). We describe an apparently adaptive epigenomic response to varied gestational choline supply in rat fetal liver and brain. S-Adenosylmethionine levels increased in both organs of E17 fetuses whose mothers consumed a choline-supplemented diet. Surprisingly, global DNA methylation increased in choline-deficient animals, and this was accompanied by overexpression of Dnmt1 mRNA. Previous studies showed that the prenatal choline supply affects the expression of multiple genes, including insulin-like growth factor 2 (Igf2), whose expression is regulated in a DNA methylation-dependent manner. The differentially methylated region 2 of Igf2 was hypermethylated in the liver of E17 choline-deficient fetuses, and this as well as Igf2 mRNA levels correlated with the expression of Dnmt1 and with hypomethylation of a regulatory CpG within the Dnmt1 locus. Moreover, mRNA expression of brain and liver Dnmt3a and methyl CpG-binding domain 2 (Mbd2) protein as well as cerebral Dnmt3l was inversely correlated to the intake of choline. Thus, choline deficiency modulates fetal DNA methylation machinery in a complex fashion that includes hypomethylation of the regulatory CpGs within the Dnmt1 gene, leading to its overexpression and the resultant increased global and gene-specific (e.g. Igf2) DNA methylation. These epigenomic responses to gestational choline supply may initiate the long term developmental changes observed in rats exposed to varied choline intake in utero. 相似文献
98.
Background
The composition of the individual eukaryote's genome and its variation within a species remain poorly defined. Even for a sequenced genome such as that of the model plant Arabidopsis thaliana accession Col-0, the large arrays of heterochromatic repeats are incompletely sequenced, with gaps of uncertain size persisting in them. 相似文献99.
John Davison Timothy J. Roper Charles J. Wilson Matthew J. Heydon Richard J. Delahay 《European Journal of Wildlife Research》2011,57(1):67-76
Examples from a variety of taxa demonstrate that under certain circumstances, the exclusion or translocation of ‘problem’
animals is ineffective in resolving human–wildlife conflicts and may even elicit new problems elsewhere. Damage caused by
badger setts (burrows) is an important source of human–wildlife conflict in the UK and is commonly managed by excluding badgers
from all or part of problem setts. We used records of licences issued for the management of such problems and a novel statistical
approach to assess spatiotemporal associations between problem cases in England from 2002 to 2005. We predicted that management
at urban badgers' setts, and particularly exclusion of badgers from urban main setts, would give rise to subsequent problems
at focal setts and in neighbouring areas. Frequencies of problems occurring at individual setts were similar in urban and
rural areas. In areas neighbouring setts subjected to management action, the background frequency of problems was higher in
urban than in rural areas, reflecting the occurrence of problems at a higher proportion of urban setts. The frequency of new
cases arising at or in the vicinity of managed setts within a critical time period after management action was not significantly
different from the background frequency of problems for any combination of land use, sett type and management approach. This
finding suggests that the measures currently employed for managing problem setts do not importantly increase the likelihood
of problems reoccurring in the same location or emerging nearby. 相似文献
100.