全文获取类型
收费全文 | 8514篇 |
免费 | 1033篇 |
出版年
2021年 | 113篇 |
2019年 | 75篇 |
2018年 | 91篇 |
2017年 | 81篇 |
2016年 | 152篇 |
2015年 | 228篇 |
2014年 | 267篇 |
2013年 | 322篇 |
2012年 | 367篇 |
2011年 | 401篇 |
2010年 | 257篇 |
2009年 | 214篇 |
2008年 | 313篇 |
2007年 | 379篇 |
2006年 | 324篇 |
2005年 | 311篇 |
2004年 | 276篇 |
2003年 | 258篇 |
2002年 | 260篇 |
2001年 | 303篇 |
2000年 | 287篇 |
1999年 | 229篇 |
1998年 | 110篇 |
1997年 | 105篇 |
1996年 | 92篇 |
1995年 | 95篇 |
1994年 | 92篇 |
1993年 | 88篇 |
1992年 | 181篇 |
1991年 | 161篇 |
1990年 | 162篇 |
1989年 | 179篇 |
1988年 | 146篇 |
1987年 | 169篇 |
1986年 | 158篇 |
1985年 | 166篇 |
1984年 | 136篇 |
1983年 | 121篇 |
1982年 | 97篇 |
1981年 | 95篇 |
1980年 | 78篇 |
1979年 | 115篇 |
1978年 | 92篇 |
1977年 | 115篇 |
1976年 | 90篇 |
1974年 | 119篇 |
1973年 | 86篇 |
1972年 | 76篇 |
1971年 | 90篇 |
1969年 | 81篇 |
排序方式: 共有9547条查询结果,搜索用时 281 毫秒
101.
Michael J. Sofia William T. Jackson Davis L. SaussyJr. Steven A. Silbaugh Larry L. Froelich Sandra L. Cockerham Peter W. Stengel 《Bioorganic & medicinal chemistry letters》1992,2(12)
A series of
-alkoxyphenols containing a tetrazole acid sidechain have been prepared as antagonists of leukotriene B4 receptors. These compounds were tested as receptor antagonists of human neutrophil and guinea pig lung membrane leukotriene B4 receptors. Compounds in this series were found to be up to 18-fold more potent than LY255283. These results indicate that the acyl group of the 1,2,4,5 substituted hydroxyacetophenone class of LTB4 antagonists is not critical to antagonist potency. 相似文献
102.
Evidence suggesting that the ARS elements associated with silencers of the yeast mating-type locus HML do not function as chromosomal DNA replication origins. 总被引:41,自引:21,他引:20 下载免费PDF全文
D D Dubey L R Davis S A Greenfeder L Y Ong J G Zhu J R Broach C S Newlon J A Huberman 《Molecular and cellular biology》1991,11(10):5346-5355
103.
Human neonates were exposed to an artificial odorant for 22h within the first two days after birth. When tested on days1618 postpartum, these infants displayed preferentialorientation to the exposure odor when paired with a novel odorant.The effects of early mere exposure on the stimulus propertiesof odors can therefore endure over a two-week interval, indicatingthat infants retain a memory trace of the exposure odor throughoutthat time period. 相似文献
104.
An osmotic lysis technique was developed to induce transient permeability in human placental microvillous membrane vesicles. The degree of vesicle opening and resealing was quantitated using the fluorescent markers, 6-carboxyfluorescein and fluorescein dextran. Compared to freeze-thaw and sonication methods, hypotonic lysis was significantly more efficient, causing greater than 90% lysis with greater than 90% subsequent resealing under optimal conditions. The transient increase in vesicle permeability permitted the unrestricted entry of macromolecules with molecular masses up to 70,000 kDa. Passive transport of water, protons, and erythritol and carrier-mediated transport of L-valine and sodium-proton exchange were unaltered by the lysis/resealing procedure. Bovine tracheal vesicles were lysed to an extent similar to placental microvillous vesicles, but rabbit renal cortical brush border and basolateral membranes were lysed to a lesser extent (approximately 60%). These results show that hypotonic lysis is a suitable method for the loading and trapping of macromolecules in isolated membrane vesicles for studies of intracellular regulation of transport. 相似文献
105.
A hybrid toxin from bacteriophage f1 attachment protein and colicin E3 has altered cell receptor specificity. 总被引:2,自引:0,他引:2 下载免费PDF全文
A hybrid protein was constructed in vitro which consists of the first 372 amino acids of the attachment (gene III) protein of filamentous bacteriophage f1 fused, in frame, to the carboxy-terminal catalytic domain of colicin E3. The hybrid toxin killed cells that had the F-pilus receptor for phage f1 but not F- cells. The activity of the hybrid protein was not dependent upon the presence of the colicin E3 receptor, BtuB protein. The killing activity was colicin E3 specific, since F+ cells expressing the colicin E3 immunity gene were not killed. Entry of the hybrid toxin was also shown to depend on the products of tolA, tolQ, and tolR which are required both for phage f1 infection and for entry of E colicins. TolB protein, which is required for killing by colicin E3, but not for infection by phage f1, was also found to be necessary for the killing activity of the hybrid toxin. The gene III protein-colicin E3 hybrid was released from producing cells into the culture medium, although the colicin E3 lysis protein was not present in those cells. The secretion was shown to depend on the 18-amino-acid-long gene III protein signal sequence. Deletion of amino acids 3 to 18 of the gene III moiety of the hybrid protein resulted in active toxin, which remained inside producing cells unless it was mechanically released. 相似文献
106.
107.
The aims of this study were to investigate the control of fatty acid synthesis and its relationship to surfactant production in the fetal lung during alteration of hormonal and substrate conditions. Lung explants from 18 day fetuses (term = 22 days) which were cultured 2 days in the presence of 10 mM lactate showed parallel acceleration of de novo fatty acid synthesis (3H2O incorporation) and [14C]choline incorporation into disaturated phosphatidylcholine (DSPC) compared to culture of explants in glucose. Both the cultured and fresh explants were resistant to the classical short term (4 h) cAMP inhibition of fatty acid synthesis with 3 mM dibutyryl cAMP or 0.5 mM aminophylline. In the cultured explants short term cAMP elevation increased DSPC production, and long term (2 day) cAMP elevation caused a further increase in DSPC synthesis and also stimulated fatty acid synthesis. In cultured explants from 17 day fetuses, dexamethasone (0.1 microM) caused a synergistic increase with aminophylline in both fatty acid synthesis and DSPC production whereas, in explants from 18 day fetuses, dexamethasone inhibited both processes and reduced the level of stimulation of DSPC and fatty acid synthesis seen with aminophylline alone. Dexamethasone also reduced the stimulation of both DSPC and fatty acid synthesis produced in the culture of 18 day explants with bacitracin (0.5 mg/ml), whereas the combination of bacitracin and aminophylline resulted in a synergistic increase in DSPC production. Culture with glucagon (0.1 microM) also stimulated DSPC synthesis but at physiological levels insulin had no effect on either DSPC or fatty acid synthesis. These data show that lung fatty acid synthesis exhibits unique features of fatty acid synthesis regulation compared to other lipogenic tissues and also suggest a link between de novo fatty acid synthesis and surfactant production during the critical period of accelerated lung maturation. 相似文献
108.
Rhizobium trifolii 0403 Is Capable of Growth in the Absence of Combined Nitrogen 总被引:1,自引:1,他引:0 下载免费PDF全文
Rhizobium trifolii 0403 was treated with 16.6 mM succinate and other nutrients and thereby induced to grow in nitrogen-free medium. The organism grew microaerophilically on either semisolid or liquid medium, fixing atmospheric nitrogen to meet metabolic needs. Nitrogen fixation was measured via 15N incorporation (18% 15N enrichment in 1.5 doublings) and acetylene reduction. Nitrogen-fixing cells had a Km for acetylene of 0.07 atm (ca. 7.09 kPa), required about 3% oxygen for optimum growth in liquid medium, and showed a maximal specific activity of 5 nmol of acetylene reduced per min per mg of protein at 0.04 atm (ca. 4.05 kPa) of acetylene. The doubling time on N-free liquid medium ranged from 1 to 5 days, depending on oxygen tension, with an optimum temperature for growth of about 30°C. Nodulation of white clover by the cultures showing in vitro nitrogenase activity indicates that at least part of the population maintained identity with wild-type strain 0403. 相似文献
109.
S Burman J C Davis M J Weber B A Averill 《Biochemical and biophysical research communications》1986,136(2):490-497
Reaction of the reduced (pink) form of the purple acid phosphatase from beef spleen with excess phosphate at pH 5.0, monitored by optical and low temperature EPR spectroscopy and by measurement of enzymatic activity, results in parallel loss of activity and oxidation of the iron chromophore. Colorimetric and radiochemical (32P) experiments indicate the presence of one mole of tightly bound phosphate in the oxidized (purple) form of the enzyme; this phosphate is released upon reduction. Acid hydrolysis of 32P-phosphate-containing enzyme, followed by high voltage paper electrophoresis, gave no evidence for significant amounts of acid-stable phosphoamino acids. 相似文献
110.
Rats treated with ethinylestradiol (5 mg kg-1 day-1 for 5 days) secrete de novo synthesized bile acids at a markedly reduced rate (-57%). Administration of the nonionic detergent Triton WR-1339 to estradiol-treated rats rapidly restored the rate of secretion of de novo synthesized bile acids to control levels. In contrast, when Triton was administered to control rats, the secretion rate of bile acids was unaffected. The reduction in bile acid synthesis displayed by estradiol-treated rats was similar to the 50% decrease in the activity of hepatic microsomal 7 alpha-hydroxylase. The activity of 7 alpha-hydroxylase was also restored to control levels by the administration of Triton to estradiol-treated rats. We examined the possibility that estradiol acts directly on the hepatic microsomes. Adding increasing amounts of estradiol to microsomes obtained from control rats resulted in decreasing activities of 7 alpha-hydroxylase. The inhibition by estradiol of 7 alpha-hydroxylase obtained in vitro occurred with amounts of estradiol that were found to accumulate in the liver via in vivo treatment. Double-reciprocal analysis showed that at and below 50 micrograms of estradiol/0.5 mg of protein uncompetitive inhibition was displayed. Additional experiments showed that adding Triton to microsomes obtained from estradiol-treated rats increased the activity of 7 alpha-hydroxylase to control levels. In contrast, Triton did not increase the activity of 7 alpha-hydroxylase when it was added to control microsomes. These data show for the first time that the estrogenic steroid estradiol acts directly on the microsomes and inhibits both the activity of 7 alpha-hydroxylase and the rate of bile acid synthesis.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献