全文获取类型
收费全文 | 781873篇 |
免费 | 88746篇 |
国内免费 | 1011篇 |
出版年
2016年 | 8612篇 |
2015年 | 12619篇 |
2014年 | 14827篇 |
2013年 | 20614篇 |
2012年 | 23554篇 |
2011年 | 24172篇 |
2010年 | 16082篇 |
2009年 | 14795篇 |
2008年 | 21379篇 |
2007年 | 22252篇 |
2006年 | 21022篇 |
2005年 | 20225篇 |
2004年 | 20169篇 |
2003年 | 18995篇 |
2002年 | 18696篇 |
2001年 | 33551篇 |
2000年 | 33971篇 |
1999年 | 27190篇 |
1998年 | 9742篇 |
1997年 | 9959篇 |
1996年 | 9367篇 |
1995年 | 8959篇 |
1994年 | 8669篇 |
1993年 | 8689篇 |
1992年 | 22466篇 |
1991年 | 22061篇 |
1990年 | 21537篇 |
1989年 | 20838篇 |
1988年 | 19535篇 |
1987年 | 18546篇 |
1986年 | 17611篇 |
1985年 | 17605篇 |
1984年 | 14582篇 |
1983年 | 12556篇 |
1982年 | 9704篇 |
1981年 | 9001篇 |
1980年 | 8232篇 |
1979年 | 13918篇 |
1978年 | 11321篇 |
1977年 | 10236篇 |
1976年 | 9603篇 |
1975年 | 10975篇 |
1974年 | 12036篇 |
1973年 | 11823篇 |
1972年 | 10804篇 |
1971年 | 9900篇 |
1970年 | 8459篇 |
1969年 | 8342篇 |
1968年 | 7624篇 |
1967年 | 6670篇 |
排序方式: 共有10000条查询结果,搜索用时 5 毫秒
31.
P C de Visser N M A J Kriek P A V van Hooft A Van Schepdael D V Filippov G A van der Marel H S Overkleeft J H van Boom D Noort 《The journal of peptide research》2003,61(6):298-306
As part of a program towards the development of novel antibiotics, a convenient method for solid-phase synthesis of the cyclic cationic peptide polymyxin B1 and analogues thereof is described. The methodology, based on cleavage-by-cyclization using Kenner's safety-catch linker, yields crude products with purities ranging from 37-67%. Antibacterial assays revealed that analogues 23-26, in which the (S)-6-methyloctanoic acid moiety is replaced with shorter acyl chains, exhibit distinct antimicrobial activity. The results suggest that the length of the acyl chain is rather critical for antimicrobial activity. On the other hand, substitution of the hydrophobic ring-segment D-Phe-6/Leu-7 in polymyxin B1 with dipeptide mimics (i.e. analogues 27-33) resulted in almost complete loss of antimicrobial activity. 相似文献
32.
Localization of cathepsin D in rat liver. Immunocytochemical study using post-embedding immunoenzyme and protein A-gold techniques 总被引:1,自引:0,他引:1
Light and electron microscopic localization of cathepsin D in rat liver was investigated by post-embedding immunoenzyme and protein A-gold techniques. By light microscopy, cytoplasmic granules of parenchymal cells and Kupffer cells were stained for cathepsin D. Weak staining was also noted in sinusoidal endothelial cells. In the parenchymal cells many of positive granules located around bile canaliculi. In the Kupffer cells and the endothelial cells, diffuse staining was noted in the cytoplasm in addition to granular staining. By electron microscopy, gold particles representing the antigenic sites for cathepsin D were seen in typical secondary lysosomes and some multivesicular bodies of the parenchymal cells and Kupffer cells. The lysosomes of the endothelial cells and fat-storing cells were weakly labeled. Quantitative analysis of the labeling density in the lysosomes of these three types of cells demonstrated that the lysosomes of parenchymal cells and Kupffer cells are main containers of cathepsin D in rat liver. The results suggest that cathepsin D functions in the intracellular digestive system of parenchymal cells and Kupffer cells but not so much in that of the endothelial cells. 相似文献
33.
34.
35.
Experiments on conscious rabbits were made to elaborate motor conditioned reflexes through pairing stimuli with electrocutaneous reinforcement applied every 30 s. Neuronal activity in the sensorimotor cortex and putamen was recorded during formation and reproduction of the conditioned reflexes before and after haloperidol injection (0.2 mg/kg i. v.). In the putamen, haloperidol increased the number of neurons exhibiting trace conditioned activity and made the intensity and duration of these processes rise. The changes seen in the sensorimotor cortex were opposite in nature. Inhibition of trace conditioned activity in the sensorimotor cortex depended mainly on the decreased amplitude of the reaction conditioned component. The role of the dopaminergic system in the interaction of the neostriatum and sensorimotor cortex and in formation and reproduction of trace conditioned activity of both the structures is discussed. 相似文献
36.
37.
38.
Results are presented on the analysis of three-dimensional motion of compound cilia or cirri in voltage-clamped specimens of the protozoan Stylonychia mytilus. Time series of three-dimensional data were obtained by using the anaxial illumination method for simultaneous recording of stereoscopic video images. Data processing involved the following steps: determination of a reference coordinate system based solely on features present in each stereo-pair; tracing of cirral axes in digitized images, conversion to parameter curves by means of least-squares polynomial approximation, conversion of pairs of two-dimensional data to a series of three-dimensional data; correction for distortion due to projective shortening and conversion to a series of polynomial triplets, and analysis of the periodical components of the motion pattern in the frequency domain. Reconstructed beating cycles show typical differences between hyperpolarization-induced ciliary activity and depolarization-induced ciliary activity. Reconstructions of the motion of the basal segment of a cirrus are in agreement with existing data. Analysis of the curvature and torsion of a cirral axis during beating does not reveal any simple pattern of propagated activity within the axoneme. The return stroke may be subdivided into two phases. First, a curvature peak develops proximally. Secondly, a region with increased torsion arises more distally and spreads out in proximal direction. Both curvature and torsion return to minimal values by the beginning of the power stroke. 相似文献
39.
40.
U Mura A M Osman A S Mohamed D Di Martino P L Ipata 《Comparative biochemistry and physiology. B, Comparative biochemistry》1987,87(1):157-160
The preservation of purine ring as purine bases appears to be a common feature of camel liver. Hepatic guanine appears to be actively converted into GMP in the camel rather than further degraded. The limiting step of guanine degradation appears to be the lack of hepatic guanase activity. Higher purine bases over uric acid ratios were found in camel urine with respect to those of zebu. 相似文献