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241.
Pyrophosphate and Ethylenediaminetetraacetate as Relaxants for Lower Invertebrates Prior to Fixation
The method is based on the use of substances that can stop muscular contraction, such as chelants of calcium (EDTA) or known plasticizer of muscle (such as Na4P2O7). Saturated aqueous Na4P2O7 solution and 0.01 M EDTA were used at a room temperature of 22 C. A standard time of 3 hr was required for the substance to have full effect. The method was successfully tested on bdelloid rotifers and subsequently on rotifers other than bdelloid, gastrotrichs, tardigrades, amoebae and vorticellids. 相似文献
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Christian Viskov Stefano Elli Elena Urso Davide Gaudesi Pierre Mourier Frederic Herman Christian Boudier Benito Casu Giangiacomo Torri Marco Guerrini 《The Journal of biological chemistry》2013,288(36):25895-25907
The antithrombin (AT) binding properties of heparin and low molecular weight heparins are strongly associated to the presence of the pentasaccharide sequence AGA*IA (ANAc,6S-GlcUA-ANS,3,6S-I2S-ANS,6S). By using the highly chemoselective depolymerization to prepare new ultra low molecular weight heparin and coupling it with the original separation techniques, it was possible to isolate a polysaccharide with a biosynthetically unexpected structure and excellent antithrombotic properties. It consisted of a dodecasaccharide containing an unsaturated uronate unit at the nonreducing end and two contiguous AT-binding sequences separated by a nonsulfated iduronate residue. This novel oligosaccharide was characterized by NMR spectroscopy, and its binding with AT was determined by fluorescence titration, NMR, and LC-MS. The dodecasaccharide displayed a significantly increased anti-FXa activity compared with those of the pentasaccharide, fondaparinux, and low molecular weight heparin enoxaparin. 相似文献
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Anna Lange-Consiglio Stefano Tassan Bruna Corradetti Aurora Meucci Roberta Perego Davide Bizzaro Fausto Cremonesi 《Cytotherapy》2013,15(8):1011-1020
Background aimsThis is the first study to compare the treatment of horse tendon and ligament injuries with the use of mesenchymal stromal cells (MSCs) obtained from two different sources: amniotic membrane (AMSCs) and bone marrow (BM-MSCs). The objective was to prove the ability of AMSCs to exert beneficial effects in vivo.MethodsFive million allogeneic frozen-thawed AMSCs or autologous fresh BM-MSCs were injected intralesionally in horses belonging to group A (51 horses) and group B (44 horses). The interval lesion/implantation was of 6–15 days for the AMSCs and 16–35 days for the BM-MSCs. Healing was assessed clinically and ultrasonographically. Follow-up was monitored for 2 further years from return to full work.ResultsNo significant adverse effects after MSCs treatment were seen in any of the horses studied, independent of the type of stromal cell implanted. All animals belonging to group A resumed their activities between 4–5 months after treatment, whereas animals of group B resumed their activities after 4–12 months. The rate of re-injury in horses treated with AMSCs is lower (4.00%) compared with the average observed when horses were treated with BM-MSCs (23.08%).ConclusionsThe possibility to inject allogeneic AMSCs in real time, before any ultrasonographic change occurs within the injured tendon and ligament, together with the higher plasticity and proliferative capacity of these cells compared with BM-MSCs, represents the main features of interest for this novel approach for the treatment of equine tendon diseases. An obvious active proliferative healing in the area injected with AMSCs makes these cells more effective than BM-MSCs. 相似文献
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Monda Hiarhi Cozzolino Vincenza Vinci Giovanni Drosos Marios Savy Davide Piccolo Alessandro 《Plant and Soil》2018,429(1-2):407-424
Plant and Soil - The use of composted agricultural wastes as source of biostimulant compounds provides an added value to the recycling of biomasses. This study aims to expand the knowledge on the... 相似文献
247.
Altered modulation of lamin A/C‐HDAC2 interaction and p21 expression during oxidative stress response in HGPS 下载免费PDF全文
Elisabetta Mattioli Davide Andrenacci Cecilia Garofalo Sabino Prencipe Katia Scotlandi Daniel Remondini Davide Gentilini Anna Maria Di Blasio Sergio Valente Emanuela Scarano Lucia Cicchilitti Giulia Piaggio Antonello Mai Giovanna Lattanzi 《Aging cell》2018,17(5)
Defects in stress response are main determinants of cellular senescence and organism aging. In fibroblasts from patients affected by Hutchinson–Gilford progeria, a severe LMNA‐linked syndrome associated with bone resorption, cardiovascular disorders, and premature aging, we found altered modulation of CDKN1A, encoding p21, upon oxidative stress induction, and accumulation of senescence markers during stress recovery. In this context, we unraveled a dynamic interaction of lamin A/C with HDAC2, an histone deacetylase that regulates CDKN1A expression. In control skin fibroblasts, lamin A/C is part of a protein complex including HDAC2 and its histone substrates; protein interaction is reduced at the onset of DNA damage response and recovered after completion of DNA repair. This interplay parallels modulation of p21 expression and global histone acetylation, and it is disrupted by LMNAmutations leading to progeroid phenotypes. In fact, HGPS cells show impaired lamin A/C‐HDAC2 interplay and accumulation of p21 upon stress recovery. Collectively, these results link altered physical interaction between lamin A/C and HDAC2 to cellular and organism aging. The lamin A/C‐HDAC2 complex may be a novel therapeutic target to slow down progression of progeria symptoms. 相似文献
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Contribution of crop model structure,parameters and climate projections to uncertainty in climate change impact assessments 总被引:2,自引:0,他引:2 下载免费PDF全文
Fulu Tao Reimund P. Rötter Taru Palosuo Carlos Gregorio Hernández Díaz‐Ambrona M. Inés Mínguez Mikhail A. Semenov Kurt Christian Kersebaum Claas Nendel Xenia Specka Holger Hoffmann Frank Ewert Anaelle Dambreville Pierre Martre Lucía Rodríguez Margarita Ruiz‐Ramos Thomas Gaiser Jukka G. Höhn Tapio Salo Roberto Ferrise Marco Bindi Davide Cammarano Alan H. Schulman 《Global Change Biology》2018,24(3):1291-1307
Climate change impact assessments are plagued with uncertainties from many sources, such as climate projections or the inadequacies in structure and parameters of the impact model. Previous studies tried to account for the uncertainty from one or two of these. Here, we developed a triple‐ensemble probabilistic assessment using seven crop models, multiple sets of model parameters and eight contrasting climate projections together to comprehensively account for uncertainties from these three important sources. We demonstrated the approach in assessing climate change impact on barley growth and yield at Jokioinen, Finland in the Boreal climatic zone and Lleida, Spain in the Mediterranean climatic zone, for the 2050s. We further quantified and compared the contribution of crop model structure, crop model parameters and climate projections to the total variance of ensemble output using Analysis of Variance (ANOVA). Based on the triple‐ensemble probabilistic assessment, the median of simulated yield change was ?4% and +16%, and the probability of decreasing yield was 63% and 31% in the 2050s, at Jokioinen and Lleida, respectively, relative to 1981–2010. The contribution of crop model structure to the total variance of ensemble output was larger than that from downscaled climate projections and model parameters. The relative contribution of crop model parameters and downscaled climate projections to the total variance of ensemble output varied greatly among the seven crop models and between the two sites. The contribution of downscaled climate projections was on average larger than that of crop model parameters. This information on the uncertainty from different sources can be quite useful for model users to decide where to put the most effort when preparing or choosing models or parameters for impact analyses. We concluded that the triple‐ensemble probabilistic approach that accounts for the uncertainties from multiple important sources provide more comprehensive information for quantifying uncertainties in climate change impact assessments as compared to the conventional approaches that are deterministic or only account for the uncertainties from one or two of the uncertainty sources. 相似文献
250.
Davide Facchinelli Gessica Marchesini Gianpaolo Nadali Livio Pagano 《Current fungal infection reports》2018,12(4):179-186