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951.
A determination of some of the factors that predict the outcome of contests between male tree lizards, Urosaurus ornatus, was made using logistic regression modelling on matched-pair data. Two-day-long encounters were staged between pairs of males differing in size (snout-vent length and mass), previous contest status (previous winners and previous losers), and coloration (dorsal coloration during their previous contest and throat coloration, a fixed trait). Mass proved to be the best single predictor of contest outcome, resulting in an 80% correct classification rate for predicting winners and losers, far better than the less than 57% correct classification rate for snout-vent length. Previous social status (winner or loser) also was a powerful single predictor of contest outcome with a 793% correct classification rate, as was previous dorsal coloration (76.7%). When combined, mass and previous status produced the strongest combination of predictors with a better than 86% correct classification rate. Contrary to several previous studies, which implicated throat coloration as an important status signal of dominance, our results failed to show that throat coloration is a strong predictor of contest outcome. Possible reasons for this discrepancy with earlier findings are discussed. The logistic regression models also allow prediction of the magnitude of difference in mass between two contestants for there to be an equal chance of winning, given a second asymmetry in contest predictors. 相似文献
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Vaughan Hurry Jan M. Anderson Murray R. Badger G. Dean Price 《Photosynthesis research》1996,50(2):159-169
We have examined tobacco transformed with an antisense construct against the Rieske-FeS subunit of the cytochromeb
6
f complex, containing only 15 to 20% of the wild-type level of cytochrome f. The anti-Rieske-FeS leaves had a comparable chlorophyll and Photosystem II reaction center stoichiometry and a comparable carotenoid profile to the wild-type, with differences of less than 10% on a leaf area basis. When exposed to high irradiance, the anti-Rieske-FeS leaves showed a greatly increased closure of Photosystem II and a much reduced capacity to develop non-photochemical quenching compared with wild-type. However, contrary to our expectations, the anti-Rieske-FeS leaves were not more susceptible to photoinhibition than were wild-type leaves. Further, when we regulated the irradiance so that the excitation pressure on photosystem II was equivalent in both the anti-Rieske-FeS and wild-type leaves, the anti-Rieske-FeS leaves experienced much less photoinhibition than wild-type. The evidence from the anti-Rieske-FeS tobacco suggests that rapid photoinactivation of Photosystem II in vivo only occurs when closure of Photosystem II coincides with lumen acidification. These results suggest that the model of photoinhibition in vivo occurring principally because of limitations to electron withdrawal from photosystem II does not explain photoinhibition in these transgenic tobacco leaves, and we need to re-evaluate the twinned concepts of photoinhibition and photoprotection.Abbreviations Chl
chlorophyll
- DCMU
3-(3,4-dichlophenyl)-1,-dimethylurea
- Fo and Fo
minimal fluorescence when all PS II reaction centers are open in dark- and light-acclimated leaves, respectively
- Fm and Fm
maximal fluorescence when all PS II reaction centers are closed in dark- and light-acclimated leaves, respectively
- Fv
variable fluorescence (Fm-Fo) in dark acclimated leaves
- Fv
variable fluorescence (Fm-Fo) in lightacclimated leaves
- NPQ
non-photochemical quenching of fluorescence
- PS I and PS II
Photosystem I and II
- P680
primary electron donor of the reaction center of PS II
- PFD
photosynthetic flux density
- QA
primary acceptor quinone of PS II
- qp
photochemical quenching of fluorescence
- V+A+Z
violaxanthin+antheraxanthin+zeaxanthin 相似文献
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David H. Lewis Garry K. Burge David M. Schmierer Paula E. Jameson 《Physiologia plantarum》1996,98(1):179-186
The cytokinin content in fruit tissue of the kiwifruit ( Actinidia deliciosa [A. Chev.] C. F. Liang et A. R. Ferguson var. deliciosa cv. Hayward) was monitored during fruit development to identify which cytokinins were present and if they were linked with specific stages of fruit growth. Cytokinins were isolated and purified by column chromatography and high-performance liquid chromatography and quantified by radioimmunoassay. A novel HPLC step utilising an amine column was successfully introduced as a preparative step in the separation of the O - and 9-glucosides from the free bases and ribosides. The radioimmunoassay results were validated, and the different cytokinins identified, by gas chromatography-mass spectrometry. Cytokinins detected in fruit included the cytokinin free bases, zeatin and isopentenyladenine, their ribosides, nucleotides and both O - and 9-glucosides. Both qualitative and quantitative changes of the cytokinins occurred during fruit development. A decrease in cytokinin concentration occurred after anthesis (from 342 pmol g−1 fresh weight at anthesis to 41 pmol g−1 fresh weight 27 days after anthesis). A large increase in cytokinin concentration and content per fruit occurred as the fruit reached commercial maturity (to 1900 pmol g−1 fresh weight). Individual cytokinins showed quite different patterns. Zeatin, in particular, showed a peak in concentration (13 pmol g−1 fresh weight) 11 days after anthesis that correlated with the beginning of the cell division phase of fruit growth. The accumulation of cytokinin (mostly zeatin riboside or zeatin nucleotide) in mature fruit may be of significance for the postharvest storage of kiwifruit fruit. 相似文献
958.
Keith R. Roesler Linda J. Savage David K. Shintani Basil S. Shorrosh John B. Ohlrogge 《Planta》1996,198(4):517-525
Acetyl-CoA carboxylase (ACCase; EC 6.4.1.2) is a regulatory enzyme of fatty acid synthesis, and in some higher-plant plastids is a multi-subunit complex consisting of biotin carboxylase (BC), biotin-carboxyl carrier protein (BCCP), and carboxyl transferase (CT). We recently described a Nicotiana tabacum L. (tobacco) cDNA with a deduced amino acid sequence similar to that of prokaryotic BC. We here provide further biochemical and immunological evidence that this higher-plant polypeptide is an authentic BC component of ACCase. The BC protein co-purified with ACCase activity and with BCCP during gel permeation chromatography of Pisum sativum L. (pea) chloroplast proteins. Antibodies to the Ricinus communis L. (castor) BC co-precipitated ACCase activity and BCCP. During castor seed development, ACCase activity and the levels of BC and BCCP increased and subsequently decreased in parallel, indicating their coordinate regulation. The BC protein comprised about 0.8% of the soluble protein in developing castor seed, and less than 0.05% of the protein in young leaf or root. Polypeptides cross-reacting with antibodies to castor BC were detected in several dicotyledons and in the monocotyledons Hemerocallis fulva L. (day lily), Iris L., and Allium cepa L. (onion), but not in the Gramineae species Hordeum vulgare L. (barley) and Panicum virgatum L. (switchgrass). The castor endosperm and pea chloroplast ACCases were not significantly inhibited by long-chain acyl-acyl carrier protein, free fatty acids or acyl carrier protein. The BC polypeptide was detected throughout Brassica napus L. (rapeseed) embryo development, in contrast to the multi-functional ACCase isoenzyme which was only detected early in development. These results firmly establish the identity of the BC polypeptide in plants and provide insight into the structure, regulation and roles of higherplant ACCases.Abbreviations ACCase
acetyl-CoA carboxylase
- ACP
acyl carrier protein
- BC
biotin carboxylase
- BCCP
biotin carboxyl carrier protein
- CT
carboxyl transferase
- MF
multi-functional
- MS
multi-subunit
We thank our colleagues Nicki Engeseth and Vicki Eccleston for advice on fatty acid analysis and Sarah Hunter for providing the developing Iris seed. This work was supported in part by grant MCB 9406466 from NSF. Acknowledgement is also made to the Michigan Agriculture Experiment Station for its support of this research. 相似文献
959.
Summary The role of tubular mastigonemes in the reversal of thrust of the anterior flagellum ofPhytophthora cinnamomi was analysed using mastigoneme-specific monoclonal antibodies and immunoflu-orescence and video microscopy. Exposure of live zoospores ofP. cinnamomi to the mastigoneme-specific Zg antibodies caused alterations in the arrangement of mastigonemes on the flagellar surface and at Zg concentrations above 0.3 /ml, mastigonemes became detached from the flagellum. As a consequence of antibody binding to the mastigonemes there were concentration-dependent perturbations in zoospore swimming behaviour and anterior flagellum beat pattern. With increasing antibody concentration zoospores swam more slowly and other parameters of their swimming pattern, such as the wavelength of the swimming helix and the frequency of rotation, were also reduced. The effects of Zg antibodies were specific at two levels: control immunoglobulins or antibodies that bound to other flagellar surface components did not have an effect on motility, and Zg antibodies did not interfere with the motility of zoospores of oomycete species to which they did not bind. The effects of antibody-induced disruption of mastigoneme arrangement strongly support previous hypotheses that tubular mastigonemes are responsible for thrust reversal by the anterior flagellum, enabling it to pull the cell through the surrounding medium. 相似文献
960.