全文获取类型
收费全文 | 278969篇 |
免费 | 16352篇 |
国内免费 | 954篇 |
专业分类
296275篇 |
出版年
2021年 | 2441篇 |
2020年 | 1500篇 |
2019年 | 1912篇 |
2018年 | 14153篇 |
2017年 | 12763篇 |
2016年 | 10918篇 |
2015年 | 6041篇 |
2014年 | 6610篇 |
2013年 | 8355篇 |
2012年 | 14175篇 |
2011年 | 22327篇 |
2010年 | 18212篇 |
2009年 | 13976篇 |
2008年 | 18035篇 |
2007年 | 19480篇 |
2006年 | 8436篇 |
2005年 | 8327篇 |
2004年 | 8607篇 |
2003年 | 8328篇 |
2002年 | 7650篇 |
2001年 | 5584篇 |
2000年 | 5003篇 |
1999年 | 4225篇 |
1998年 | 2319篇 |
1997年 | 1919篇 |
1996年 | 1727篇 |
1995年 | 1586篇 |
1994年 | 1467篇 |
1993年 | 1577篇 |
1992年 | 3109篇 |
1991年 | 3041篇 |
1990年 | 3015篇 |
1989年 | 2738篇 |
1988年 | 2577篇 |
1987年 | 2450篇 |
1986年 | 2254篇 |
1985年 | 2419篇 |
1984年 | 2173篇 |
1983年 | 1917篇 |
1982年 | 1641篇 |
1981年 | 1515篇 |
1980年 | 1443篇 |
1979年 | 1815篇 |
1978年 | 1573篇 |
1977年 | 1408篇 |
1975年 | 1461篇 |
1974年 | 1602篇 |
1973年 | 1681篇 |
1972年 | 1636篇 |
1971年 | 1490篇 |
排序方式: 共有10000条查询结果,搜索用时 8 毫秒
961.
Several levels of control of elongation rate are revealed through the detailed study of responses of the Nitella internode to abrupt shifts in turgor. The immediate response, which apparently reflects the physical state of the cell, is approximately described by the equation r = (P — Y)m where r is rate, P is pressure, Y is the wall's yielding threshold, and m is related to the wall's apparent fluidity (reciprocal viscosity). Because P and Y are in the range 5 to 6 atmospheres, and (P — Y) is roughly 0.2 atmosphere, elongation rate is initially extremely sensitive to changes in P. A small step-down in turgor (0.7 atmosphere) stops growth, and a similar rise greatly accelerates it. These initial responses are, however, soon (15 minutes) compensated by changes in Y. An apparent metabolism-dependent reaction (azide-sensitive) lowers Y; strain hardening (azide-insensitive) raises it. These two opposing processes, acting on Y, serve as a governor on (P — Y), tending to maintain it at a given value despite changes in P. This ability to compensate is itself a function of turgor. Turgor step-downs are less and less well compensated, leading to lower rate, as turgor falls from 5 atmospheres to about 2 atmospheres where growth appears not to resume. This is the lowest attainable yield value, Y1. The turgor dependency of compensation reflects a turgor requirement of the Y-lowering (“wall-softening”) process. Thus the relation between steady state, rs, and turgor is an indirect one, derived from time-dependent alterations of the cell wall. This relationship superficially resembles the instantaneously valid one in that, roughly, rs = (P — Y1)ms. Y1 and ms, however, have much lower values than Y and m. The duality of the elongation rate versus turgor relation and the prominent role of Y in regulating rate are the major features of growth control in Nitella. 相似文献
962.
963.
Isolation and Genetic Analysis of a Strain of Escherichia coli K-12 with an Amber recA Mutation 总被引:11,自引:5,他引:6 下载免费PDF全文
David W. Mount 《Journal of bacteriology》1971,107(1):388-389
The isolation, properties, and genetic analysis of a strain of Escherichia coli K-12 with an amber recA mutation are described. The experiments demonstrate that the recA product is a protein that is probably not essential for growth. 相似文献
964.
Glucosylation of Teichoic Acid: Solubilization and Partial Characterization of the Uridine Diphosphoglucose:Polyglycerolteichoic Acid Glucosyl Transferase from Membranes of Bacillus subtilis 总被引:4,自引:2,他引:2 下载免费PDF全文
Polyglycerolteichoic acid:glucosyl transferase (TAG transferase), one of the three enzymes involved in the pathway leading to the glucosylation of teichoic acid in Bacillus subtilis 168, was investigated. During the early stages of the growth of B. subtilis, TAG transferase is predominantly a soluble enzyme found in the cytoplasm. As growth proceeds, the amount of soluble enzyme decreases and the proportion of insoluble, membrane-bound TAG transferase increases, reaching a maximal value at the close of the logarithmic phase. Data are presented which suggest that these are two forms of the same enzyme, or have some common component. The effects of chaotropic agents, such as sodium trichloroacetate and sodium perchlorate, on the cytoplasmic membrane were also studied. These data show that such compounds can effectively remove the TAG transferase from the membrane in a water-soluble form. A study of some of the physical properties of this solubilized enzyme suggests that there is little difference between the two forms of the enzyme. Experiments are described which indicate that the glucosyl transfer by both the membrane-bound and soluble enzymes is not mediated by lipids. 相似文献
965.
966.
967.
Summary In contrast with the toxicities of sulfate and chloride salts added to substrates, the anions SO4 and C1 were not injurious when accumulated without leaf burning by cotton and tomato plants from atmospheres enriched with
SO2 or HC1 gases. The foregoing results are discussed in terms of cationenzyme interactions which appear to represent at least
a major cause of salt toxicity. Although anions are largely unreactive with enzymes it has long been observed that chloride
salts in soil solutions are far more toxic than sulfate salts. Five of seven species have shown nearly equal growth repressions
on substrates with 100 me/1 of C1 salts versus 200 me of SO4 salts, each added as 50 per cent Na. The ion activities of the two solutions were equal and the sum of cations in the plant
saps were similar. The osmotic differentials (average about 10 atm) between the expressed tissue fluids and these substrate
solutions were remarkably uniform within species. It is projected that the downward transport of salts via the phloem provides
for root concentrations which supply ions to the xylem and thereby control the uptake of substrate salts. 相似文献
968.
The transport of abscisic acid in cotyledonary petiole sectionsof cotton seedlings was investigated using (2-14C)-ABA in adonor-receiver system. Using the intercept technique, a transportvelocity of 22.4 mm/hr was determined. This velocity was foundto be independent of the length of the petiole or of the concentrationof ABA applied. In a 24-hr period 70% of the applied ABA movedthrough the tissue sections. No polarity was found in any ofthe studies. The capacity of the transport system decreased with increasingage of the tissue and with increasing time of pre-exposure toABA. When metabolism of the tissue was restricted by cold temperature,low-O2 tension or DNP treatment, transport was inhibited 80to 98%.The distribution of ABA in the petiole during transportwas also investigated. The results are discussed in relationto the well-studied transport of IAA and in relation to thephysiologicalrole of ABA.
1 Present address: Stanford Research Institute-Irvine, 19722Jamboree Blvd., Irvine, Calif. 92664, U.S.A. (Received November 30, 1970; ) 相似文献
969.
Demonstration of an “Excitation-Contraction Recoupling” Mechanism in Mammalian Ventricular Myocardium 总被引:1,自引:0,他引:1
A PROCESS called “excitation-contraction coupling” has been generally accepted to take place only in the direction of excitation to contraction. Through this mechanism a propagated action potential initiates an active state in skeletal or cardiac muscle and the muscle contracts. We propose that, in the mammalian ventricular myocardium at least, the process is not unidirectional and an important reverse coupling between the contractile system and the excitable plasma membrane has been overlooked. Through this feedback interaction the mode of contraction (that is, isotonic or isometric) not only determines the instantaneous electrical state of the plasma membrane, but also influences the mechanical events of the subsequent beats. Thus when Kaufmann et al.1 recorded intracellular action potentials from cat papillary muscle, the time course of the repolarization was altered depending on the mode of contraction. Some kind of contraction-excitation feedback has also been suggested by Stauch2 and Lab3,4. They showed a difference in the shape of the monophasic action potential, as recorded by a suction electrode, when comparing isotonic and isovolumic contraction of the intact ventricle. But their experimental conditions did not allow satisfactory analysis of the phenomenon. 相似文献
970.
Correlated response in male and female sterility to selection for pupa weight in Tribolium castaneum
D. D. Kress F. D. Enfield O. Braskerud 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1971,41(5):197-202
Summary The correlated responses in male and female sterility to 50 generations of individual selection for pupa weight in Tribolium were analyzed. Two replicate lines (S-lines) were selected for heavier pupa weight and stabilizing selection for pupa weight was practiced in two replicate control lines (C-lines). There was close agreement between replicates in both sets of lines for direct and correlated responses. The rate of inbreeding has been constant for all lines (approximately 0.5% per generation).Regression of generation means for pupa weight on generation of selection indicated a significant linear regression in the direct response for both lines. The linear increases of 46 and 55 g. per generation in the S-lines accounted for 98% of the variation among generations and the linear decreases of 5 and 10 g. per generation in the C-lines accounted for 70–90% of the variation in the generation means.Maximum likelihood estimators were used to calculate the frequency of male and female sterility for each generation and line. Average sterility in the base population ranged from about 4 to 12% for both sexes. Polynomial regressions of percent sterility on generation of selection showed that quadratic and higher order regressions were occasionally significant but accounted for a relatively small fraction of the total variation. In the two S-line replicates, linear regression coefficients of percent sterility on generation number were 0.16±.09 and 0.20±.07 for males and 0.72±.08 and 0.54±.08 for females, suggesting a larger correlated response in female than in male sterility. In the C-lines, linear regression coefficients were 0.02±.08 and –.12±.05 for males in the two replicates and –.05±.05 and –.05±.05 for females. Estimates of realized genetic correlations between pupa weight and sterility in the S-lines ranged from 0.04 to 0.14 for males and from 0.14 to 0.37 for females when the heritability of sterility was allowed to take on values from 0.05 to 0.25.Supported by NSF Grants G-1238 and GB-5987, NIH Grant GM-16074 and NIH Fellowship 1 FO2 GM4 5130-01. 相似文献