全文获取类型
收费全文 | 175329篇 |
免费 | 10444篇 |
国内免费 | 90篇 |
专业分类
185863篇 |
出版年
2021年 | 1665篇 |
2020年 | 982篇 |
2019年 | 1248篇 |
2018年 | 2387篇 |
2017年 | 2418篇 |
2016年 | 4896篇 |
2015年 | 9682篇 |
2014年 | 9813篇 |
2013年 | 10345篇 |
2012年 | 11455篇 |
2011年 | 8647篇 |
2010年 | 6261篇 |
2009年 | 6008篇 |
2008年 | 6290篇 |
2007年 | 6288篇 |
2006年 | 5940篇 |
2005年 | 11617篇 |
2004年 | 10199篇 |
2003年 | 8275篇 |
2002年 | 5656篇 |
2001年 | 2020篇 |
2000年 | 1022篇 |
1999年 | 2452篇 |
1998年 | 1608篇 |
1997年 | 1042篇 |
1995年 | 915篇 |
1992年 | 2576篇 |
1991年 | 2565篇 |
1990年 | 2632篇 |
1989年 | 2538篇 |
1988年 | 2453篇 |
1987年 | 2278篇 |
1986年 | 2055篇 |
1985年 | 2259篇 |
1984年 | 1766篇 |
1983年 | 1449篇 |
1982年 | 1176篇 |
1981年 | 1078篇 |
1980年 | 1027篇 |
1979年 | 1457篇 |
1978年 | 1224篇 |
1977年 | 1002篇 |
1976年 | 1026篇 |
1975年 | 1173篇 |
1974年 | 1345篇 |
1973年 | 1350篇 |
1972年 | 1163篇 |
1971年 | 1111篇 |
1970年 | 993篇 |
1969年 | 999篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
951.
Summary Microbodies are ubiquitous organelles in fungal cells, occurring in both vegetative hyphae and spores. They are bounded by a single membrane and may contain a crystalloid inclusion with subunits spaced at regular intervals. Typically, they contain catalase which reacts with the cytochemical stain 3,3-diaminobenzidine to yield an electron-opaque product, urate oxidase,l--hydroxy acid oxidase andd-amino acid oxidase. Their fragility and the necessity to disrupt the tough fungal cell wall before isolating them make them difficult to isolate. Analysis of enzymes in purified or partially purified microbodies from fungi indicates that they participate in fatty acid degradation, the glyoxylate cycle, purine metabolism, methanol oxidation, assimilation of nitrogenous compounds, amine metabolism and oxalate synthesis. In organisms where microbodies are known to contain enzymes of the glyoxylate cycle, they are known as glyoxysomes; where they are known to contain peroxidatic activity, they are known as peroxisomes. In some cases microbodies contain enzymes for only a portion of a pathway or cycle. Thus, they must be involved in metabolic cooperation with other organelles, particularly mitochondria. The number, size and shape of microbodies in cells, their buoyant density and their enzyme contents may vary with the composition of the medium; their proliferation in cells is regulated by the growth environment. The isolation from the same organism of microbodies with different buoyant densities and different enzymes suggests strongly that more than one type of microbody can be formed by fungi. 相似文献
952.
Photodynamic and non-photodynamic action of several porphyrins on the activity of some heme-enzymes 总被引:1,自引:0,他引:1
S G Afonso S Chinarro J J Mu?oz R E de Salamanca A M Batlle 《Journal of enzyme inhibition》1990,3(4):303-310
The action of porphyrins, uroporphyrin I and III (URO I and URO III), pentacarboxylic porphyrin I (PENTA I), coproporphyrin I and III (COPRO I and COPRO III), protoporphyrin IX (PROTO IX) and mesoporphyrin (MESO), on the activity of human erythrocytes delta-aminolevulinic acid dehydratase, porphobilinogenase, deaminase and uroporphyrinogen decarboxylase in the dark and under UV light was investigated. Both photoinactivation and light-independent inactivation was found in all four enzymes using URO I as sensitizer. URO III had a similar action as URO I on porphobilinogenase and deaminase and PROTO IX exerted equal effect as URO I on delta-aminolevulinic acid dehydratase and uroporphyrinogen decarboxylase. Photodynamic efficiency of the porphyrins was dependent on their molecular structure. Selective photodecomposition of enzymes by URO I, greater specificity of tumor uptake by URO I and enhanced porphyrin synthesis by tumors from delta-aminolevulic acid, with predominant formation of URO I, underline the possibility of using URO I in detection of malignant cells and photodynamic therapy. 相似文献
953.
Ana M. Moura-da-Silva R. David G. Theakston Julian M. Cramptonl 《Journal of molecular evolution》1996,43(3):263-269
The evolution of the Metalloproteinase Disintegrin Cysteine-rich (MDC) gene family and that of the mammalian Matrix-degrading
Metalloproteinases (MMPs) are compared. The alignment of snake venom and mammalian MDC and MMP precursor sequences generated
a phylogenetic tree that grouped these proteins mainly according to their function. Based on this observation, a common ancestry
is suggested for mammalian and snake venom MDCs; it is also possible that gene duplication of the already-assembled domain
structure, followed by divergence of the copies, may have significantly contributed to the evolution of the functionally diverse
MDC proteins. The data also suggest that the structural resemblance of the zinc-binding motif of venom MDCs and MMPs may best
be explained by common ancestry and conservation of the proteolytic motifs during the divergence of the proteins rather than
through convergent evolution.
Correspondence to: J.M. Crampton 相似文献
954.
L C Sellin K Mattila A Annila J J Schmidt J J McArdle M Hyv?nen T T Rantala T Kivist? 《Biophysical journal》1996,70(1):3-13
The 22-residue toxic peptide (WTX1) from the venom of the Southeast Asian snake Trimeresurus wagleri has multiple sites of action, but its lethal effect has been attributed to blocking the postsynaptic acetylcholine receptor at the neuromuscular junction. The 3-dimensional structure of WTX1 was studied using 2-dimensional nuclear magnetic resonance spectroscopy, circular dichroism, and computer simulations. In aqueous solution, WTX1 was shown to have extended and flexible "tails" defined by a short, rigid disulfide-bonded loop. The flexible regions can undergo structural rearrangement when moved from an aqueous to a less polar environment and may contribute to its effectiveness at different receptor sites. By substituting Gly or Phe for His at position 10, significant effects on the disulfide bond formation and, thereby, the activity of the peptide were observed. These results suggest that even subtle differences in single residues can have profound effects on the dynamics of folding, disulfide bond formation, and activity of this toxic peptide. 相似文献
955.
956.
David R. Thompson Jurgen E. Pohl Yiu-Yu S. Tse Robert W. Hiorns 《International journal of biometeorology》1996,39(3):116-120
Analysis of the time of onset of chest pain in 2254 patients with a myocardial infarction admitted to a coronary care unit in Leicester during a 10-year period shows an association with temperature and humidity. During both the most cold and humid times of the year, the relationship is a strong one. A generalized linear model with a log link was used to fit the data and the backward elimination selection procedure suggested a humid, cold day might help to trigger the occurrence of myocardial infarction. In addition, cold weather was found to have a stronger effect on the male population while those men aged between 50 and 70 years were more sensitive to the effect of high humidity. 相似文献
957.
Hydrilla verticillata (L. f.) Royle tubers from monoecious plants andPotamogeton gramineus L. winter buds were sprouted and allowed to grow in the dark for 120 days. We measured plant length and counted the number
of leaves at 2–3 day intervals.Hydrilla grew most rapidly during the first 16–17 days andPotamogeton grew most rapidly during the first 16–25 days. Measurement of propagule carbon content over time indicated that cessation
of rapid growth coincided with depletion of tuber carbon by one-half forHydrilla. ForPotamogeton, growth was reduced after 16 to 25 days while the winter bud C half-life was 37 days. Calculations indicated thatHydrilla mobilized 49% andPotamogeton 39% of the initial propagule carbon to support growth. In a second experiment, in which plants were grown in substrate the
plants grew taller and produced slightly more leaves per plant.Potamogeton removed from darkness after specified time periods, and allowed to grow for 21 days in a greenhouse recovered from 20–30
days in the dark. Similarly treatedHydrilla plants recovered from up to 80 days in the dark.Potamogeton had mobilized 79% of initial C by the time it was unable to recover from the dark treatment. Combined results for both species
indicate that the majority of propagule C was utilized in the first 16 to 30 days following sprouting. In conjunction with
an understanding propagule sprouting requirements, this information will be useful in the timing of application for management
techniques.
The U.S. Government right to retain a non-exclusive, royalty-free licence in and to any copyright is acknowledged.
The U.S. Government right to retain a non-exclusive, royalty-free licence in and to any copyright is acknowledged. 相似文献
958.
959.
Summary The role of tubular mastigonemes in the reversal of thrust of the anterior flagellum ofPhytophthora cinnamomi was analysed using mastigoneme-specific monoclonal antibodies and immunoflu-orescence and video microscopy. Exposure of live zoospores ofP. cinnamomi to the mastigoneme-specific Zg antibodies caused alterations in the arrangement of mastigonemes on the flagellar surface and at Zg concentrations above 0.3 /ml, mastigonemes became detached from the flagellum. As a consequence of antibody binding to the mastigonemes there were concentration-dependent perturbations in zoospore swimming behaviour and anterior flagellum beat pattern. With increasing antibody concentration zoospores swam more slowly and other parameters of their swimming pattern, such as the wavelength of the swimming helix and the frequency of rotation, were also reduced. The effects of Zg antibodies were specific at two levels: control immunoglobulins or antibodies that bound to other flagellar surface components did not have an effect on motility, and Zg antibodies did not interfere with the motility of zoospores of oomycete species to which they did not bind. The effects of antibody-induced disruption of mastigoneme arrangement strongly support previous hypotheses that tubular mastigonemes are responsible for thrust reversal by the anterior flagellum, enabling it to pull the cell through the surrounding medium. 相似文献
960.
Vlastimil K?ivan 《Theoretical population biology》1996,49(3):265-290
A system consisting of a population of predators and two types of prey is considered. The dynamics of the system is described by differential equations with controls. The controls model how predators forage on each of the two types of prey. The choice of these controls is based on the standard assumption in the theory of optimal foraging which requires that each predator maximizes the net rate of energy intake during foraging. Since this choice depends on the densities of populations involved, this allows us to link the optimal behavior of an individual with the dynamics of the whole system. Simple qualitative analysis and some simulations show the qualitative behavior of such a system. The effect of the optimal diet choice on the stability of the system is discussed. 相似文献