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991.
Previous works of our group demonstrated that xenobiotic metabolism by brain microsomes or cultured cerebral cells may promote the formation of reactive oxygen species. In order to characterise the risk of oxidative stress to both the central nervous system and the blood-brain barrier, we measured in the present work the release of superoxide in the culture medium of rat cerebrovascular endothelial cells during the metabolism of menadione, anthraquinone, diquat or nitrofurazone. Assays were run in the same experimental conditions on primary cultures of rat neurones and astrocytes. Quinone metabolism efficiently produced superoxide, but the production of radicals during the metabolism of diquat or nitrofurazone was very low, as a probable result of their reduced transport inside the cells. In all cell types assayed, superoxide production was time- and concentration-dependent, and cultured astrocytes always produced the highest amounts of radicals. Superoxide formation by microsomes prepared from the cultured cells was decreased by immunoinhibition of NADPH-cytochrome P450 reductase or by its irreversible inhibition by diphenyliodonium chloride, suggesting the involvement of this flavoprotein in radical production. Cerebrovascular endothelial cells cultured on collagen-coated filters produced equivalent amounts of superoxide both at their luminal side and through the artificial basement membrane, suggesting that in vivo, endothelial superoxide production may endanger adjacent astrocytes and neurones. 相似文献
992.
Lièvre V Becuwe P Bianchi A Koziel V Franck P Schroeder H Nabet P Dauça M Daval JL 《Free radical biology & medicine》2000,29(12):1291-1301
993.
Patel HG Aras RV Dave KR Katyare SS 《Zeitschrift für Naturforschung. C, Journal of biosciences》2000,55(9-10):770-777
Kinetic properties of Na+, K+ ATPase of membranes from rat and human erythrocytes were examined. The enzyme stability decreased with incubation time. The Vmax of the human enzyme was about 4 times lower than the values of the rat enzyme. However the energies of activation were higher. Phase transition temperature for the rat and the human enzyme was 24 degrees C and 17 degrees C, respectively. The human erythrocyte membranes were characterized by lower total phospholipid and cholesterol contents and were relatively more fluid. The human membranes contained lower proportions of acidic phospholipids which correlated well with the lower Vmax of the enzyme; the proportion of lysophosphoglyceride and sphingomyelin was higher in the human membrane. 相似文献
994.
Wei?WuEmail author Nilesh?Dave George?C?Tseng Thomas?Richards Eric?P?Xing Naftali?Kaminski 《BMC bioinformatics》2005,6(1):309
Background
The quality of microarray data can seriously affect the accuracy of downstream analyses. In order to reduce variability and enhance signal reproducibility in these data, many normalization methods have been proposed and evaluated, most of which are for data obtained from cDNA microarrays and Affymetrix GeneChips. CodeLink Bioarrays are a newly emerged, single-color oligonucleotide microarray platform. To date, there are no reported studies that evaluate normalization methods for CodeLink Bioarrays. 相似文献995.
Background
Cyclic nucleotides are ubiquitous intracellular messengers. Until recently, the roles of cyclic nucleotides in plant cells have proven difficult to uncover. With an understanding of the protein domains which can bind cyclic nucleotides (CNB and GAF domains) we scanned the completed genomes of the higher plants Arabidopsis thaliana (mustard weed) and Oryza sativa (rice) for the effectors of these signalling molecules. 相似文献996.
997.
Amar P Ballet P Barlovatz-Meimon G Benecke A Bernot G Bouligand Y Bourguine P Delaplace F Delosme JM Demarty M Fishov I Fourmentin-Guilbert J Fralick J Giavitto JL Gleyse B Godin C Incitti R Képès F Lange C Le Sceller L Loutellier C Michel O Molina F Monnier C Natowicz R Norris V Orange N Pollard H Raine D Ripoll C Rouviere-Yaniv J Saier M Soler P Tambourin P Thellier M Tracqui P Ussery D Vincent JC Vannier JP Wiggins P Zemirline A 《Acta biotheoretica》2002,50(4):357-373
998.
Myeloperoxidase functions as a major enzymatic catalyst for initiation of lipid peroxidation at sites of inflammation 总被引:7,自引:0,他引:7
Zhang R Brennan ML Shen Z MacPherson JC Schmitt D Molenda CE Hazen SL 《The Journal of biological chemistry》2002,277(48):46116-46122
Initiation of lipid peroxidation and the formation of bioactive eicosanoids are pivotal processes in inflammation and atherosclerosis. Currently, lipoxygenases, cyclooxygenases, and cytochrome P450 monooxygenases are considered the primary enzymatic participants in these events. Myeloperoxidase (MPO), a heme protein secreted by activated leukocytes, generates reactive intermediates that promote lipid peroxidation in vitro. For example, MPO catalyzes oxidation of tyrosine and nitrite to form tyrosyl radical and nitrogen dioxide ((.)NO(2)), respectively, reactive intermediates capable of initiating oxidation of lipids in plasma. Neither the ability of MPO to initiate lipid peroxidation in vivo nor its role in generating bioactive eicosanoids during inflammation has been reported. Using a model of inflammation (peritonitis) with MPO knockout mice (MPO(-/-)), we examined the role for MPO in the formation of bioactive lipid oxidation products and promoting oxidant stress in vivo. Electrospray ionization tandem mass spectrometry was used to simultaneously quantify individual molecular species of hydroxy- and hydroperoxy-eicosatetraenoic acids (H(P)ETEs), F(2)-isoprostanes, hydroxy- and hydroperoxy-octadecadienoic acids (H(P)ODEs), and their precursors, arachidonic acid and linoleic acid. Peritonitis-triggered formation of F(2)-isoprostanes, a marker of oxidant stress in vivo, was reduced by 85% in the MPO(-/-) mice. Similarly, formation of all molecular species of H(P)ETEs and H(P)ODEs monitored were significantly reduced (by at least 50%) in the MPO(-/-) group during inflammation. Parallel analyses of peritoneal lavage proteins for protein dityrosine and nitrotyrosine, molecular markers for oxidative modification by tyrosyl radical and (.)NO(2), respectively, revealed marked reductions in the content of nitrotyrosine, but not dityrosine, in MPO(-/-) samples. Thus, MPO serves as a major enzymatic catalyst of lipid peroxidation at sites of inflammation. Moreover, MPO-dependent formation of (.)NO-derived oxidants, and not tyrosyl radical, appears to serve as a preferred pathway for initiating lipid peroxidation and promoting oxidant stress in vivo. 相似文献
999.
Fowler A Swift D Longman E Acornley A Hemsley P Murray D Unitt J Dale I Sullivan E Coldwell M 《Analytical biochemistry》2002,308(2):223-231
We used two kinases, c-jun N terminal kinase (JNK-1) and protein kinase C (PKC), as model enzymes to evaluate the potential of fluorescence polarization (FP) for high-throughput screening and the susceptibility of these assays to compound interference. For JNK-1 the enzyme kinetics in the FP assay were consistent with those found in a [gamma-33P]ATP filter wash assay. Determined pIC(50)s for nonfluorescent JNK-1 inhibitors were also consistent with those found in the filter wash assay. In contrast, fluorescent compounds were found to interfere with the JNK-1 FP assay, appearing as false positives, defined by their lack of activity in the filter wash assay. We also developed a second assay using a different kinase, protein kinase C, which was used to test a 5000 compound diversity set. As for JNK-1, interference from fluorescent compounds caused a high false positive rate. The Molecular Devices Corporation 'FLARe' instrument is capable of discriminating between fluorophores on the basis of their fluorescence (excited state) lifetime, and may assist in reducing compound interference in fluorescent assays. In both model FP kinase assays described here some, although not complete, reduction in interference from fluorescent compounds was achieved by the use of FLARe. 相似文献
1000.
Barfield JP Sells PD Rowe DA Hannigan-Downs K 《Journal of strength and conditioning research / National Strength & Conditioning Association》2002,16(3):472-473
The purpose of this study was to investigate the presence of a practice effect on the Wingate anaerobic test (WAnT). Twenty-five young adult men (mean age = 20 years) performed 2 trials of the WAnT, which were separated by 7 days. Mean peak power (PP) and mean power (MP) for trials I and II were compared using a 1-way repeated measures analysis of variance to determine if a practice effect existed. Mean PP and MP scores were significantly higher (p < 0.025) on trial II (867.64 and 634.68 W for PP and MP, respectively) than on trial I (764.48 and 604.92 W), indicating that a practice effect occurred. Effect size (Cohen's d) for PP and MP was 0.72 and 0.35, indicating a large effect and small effect, respectively. Therefore, at least 1 full administration should be performed prior to a baseline power output measurement. 相似文献