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排序方式: 共有123条查询结果,搜索用时 15 毫秒
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Slingluff CL Colella TA Thompson L Graham DD Skipper JC Caldwell J Brinckerhoff L Kittlesen DJ Deacon DH Oei C Harthun NL Huczko EL Hunt DF Darrow TL Engelhard VH 《Cancer immunology, immunotherapy : CII》2000,48(12):661-672
Melanoma-reactive HLA-A*0201-restricted cytotoxic T lymphocyte (CTL) lines generated in vitro lyse autologous and HLA-matched
allogeneic melanoma cells and recognize multiple shared peptide antigens from tyrosinase, MART-1, and Pmel17/gp100. However,
a subset of melanomas fail to be lysed by these T cells. In the present report, four different HLA-A*0201+ melanoma cell lines not lysed by melanoma-reactive allogeneic CTL have been evaluated in detail. All four are deficient in
expression of the melanocytic differentiation proteins (MDP) tyrosinase, Pmel17/gp100, gp75/trp-1, and MART-1/Melan-A. This
concordant loss of multiple MDP explains their resistance to lysis by melanoma-reactive allogeneic CTL and confirms that a
subset of melanomas may be resistant to tumor vaccines directed against multiple MDP-derived epitopes. All four melanoma lines
expressed normal levels of HLA-A*0201, and all were susceptible to lysis by xenoreactive-peptide-dependent HLA-A*0201-specific
CTL clones, indicating that none had identifiable defects in antigen-processing pathways. Despite the lack of shared MDP-derived
antigens, one of these MDP-negative melanomas, DM331, stimulated an effective autologous CTL response in vitro, which was
restricted to autologous tumor reactivity. MHC-associated peptides isolated by immunoaffinity chromatography from HLA-A1 and
HLA-A2 molecules of DM331 tumor cells included at least three peptide epitopes recognized by DM331 CTL and restricted by HLA-A1
or by HLA-A*0201. Recognition of these CTL epitopes cannot be explained by defined, shared melanoma antigens; instead, unique
or undefined antigens must be responsible for the autologous-cell-specific anti-melanoma response. These findings suggest
that immunotherapy directed against shared melanoma antigens should be supplemented with immunotherapy directed against unique
antigens or other undefined antigens, especially in patients whose tumors do not express MDP.
Received: 31 October 1997 / Accepted: 4 August 1999 相似文献
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Kye Won Wang Karolyn Barker Steven Benner Tania Betancourt 《Molecular simulation》2018,44(12):1004-1015
In this paper, we develop a coarse-grained nucleotide model for the purpose of simulating large-scale aptamer-based hydrogel network formation in future research. In the model, each nucleotide is represented by a single interaction site containing sugar, phosphate and base. Discontinuous molecular dynamics (DMD) simulations are performed to simulate formation and denaturation of oligonucleotide duplexes as a function of temperature. The simulated melting temperatures of oligonucleotide duplexes are calculated in simulations of systems with different sequences, lengths and concentrations of oligonucleotides, and compared to data from the OligoAnalyzer tool. The denaturation of oligonucleotide triplexes containing a hybridised structure of three different oligonucleotides is analysed using both simulations and experiments. The nucleotide model is found to be a good predictor of the oligonucleotide’s hybridised state for both duplexes and triplexes. This coarse-grained model has wide ranging applications in the development or optimisation of DNA-based technologies including DNA origami, DNA-enabled hydrogels and DNA-based biosensors. 相似文献
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Intact chloroplasts isolated from mature leaf tissue of the homosporous fern Athyrium filixfemina were osmotically ruptured and subjected to starch gel electrophoresis in side by side comparisons with whole leaf extracts. The single enzyme activities of reportedly cytosolic [NADP]IDH and [NADP]ME were not expressed in the chloroplast fraction, and these were used as controls ensuring the cytosol-free quality of the chloroplast preparations. Isozymes F1,6DP-1, PGI-1, PGM-1, 6PGDH-1, ALDO-1, TPI-2, [NAD(P)]G3PDH-1, and [NAD(P)]G3PDH-2 are active in the chloroplast fraction, whereas Fl,6DP-2, PGI-2, PGM-2, 6PGDH-2, ALDO-2, and TPI-1 were lacking from the chloroplast fraction and are considered cytosolic. The single enzyme activities observed for AAT and SkDH, are chloroplastic. These data indicate that the two isozymes of certain enzymes in Athyrium filix-femina are not the products of duplicated loci resulting from polyploidy, but are distinct and subcellularly compartmentalized as demonstrated in heterosporous plants. Thus A. filix-femina is functionally diploid in spite of its high chromosome number of 2n = 80. 相似文献
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Anathy V Roberson E Cunniff B Nolin JD Hoffman S Spiess P Guala AS Lahue KG Goldman D Flemer S van der Vliet A Heintz NH Budd RC Tew KD Janssen-Heininger YM 《Molecular and cellular biology》2012,32(17):3464-3478
We recently demonstrated that S-glutathionylation of the death receptor Fas (Fas-SSG) amplifies apoptosis (V. Anathy et al., J. Cell Biol. 184:241-252, 2009). In the present study, we demonstrate that distinct pools of Fas exist in cells. Upon ligation of surface Fas, a separate pool of latent Fas in the endoplasmic reticulum (ER) underwent rapid oxidative processing characterized by the loss of free sulfhydryl content (Fas-SH) and resultant increases in S-glutathionylation of Cys294, leading to increases of surface Fas. Stimulation with FasL rapidly induced associations of Fas with ERp57 and glutathione S-transferase π (GSTP), a protein disulfide isomerase and catalyst of S-glutathionylation, respectively, in the ER. Knockdown or inhibition of ERp57 and GSTP1 substantially decreased FasL-induced oxidative processing and S-glutathionylation of Fas, resulting in decreased death-inducing signaling complex formation and caspase activity and enhanced survival. Bleomycin-induced pulmonary fibrosis was accompanied by increased interactions between Fas-ERp57-GSTP1 and S-glutathionylation of Fas. Importantly, fibrosis was largely prevented following short interfering RNA-mediated ablation of ERp57 and GSTP. Collectively, these findings illuminate a regulatory switch, a ligand-initiated oxidative processing of latent Fas, that controls the strength of apoptosis. 相似文献