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Andoljsek D Preloznik Zupan I Zontar D Cernelc P Mlakar U Modic M Pretnar J Zver S 《Cellular & molecular biology letters》2002,7(2):343-345
The diagnosis of acute myeloblastic leukaemia (AML) is based on cell morphology, cytogenetic and molecular changes, cell markers and clinical data. Our aim was to establish whether morphology and cell markers are comparable in the evaluation of AML. Bone marrow smears were analysed, and flow cytometry and monoclonal antibodies were used to determine cell type and maturity. Morphology and cell markers correlated differently in different AML subtypes. 相似文献
73.
Specific activation of the Bacillus quorum-sensing systems by isoprenylated pheromone variants 总被引:3,自引:0,他引:3
Natural genetic competence in Bacillus subtilis is controlled by quorum-sensing (QS). The ComP- ComA two-component system detects the signalling molecule ComX, and this signal is transduced by a conserved phosphotransfer mechanism. ComX is synthesized as an inactive precursor and is then cleaved and modified by ComQ before export to the extracellular environment. The comQXP' loci of a set of natural Bacillus isolates have been sequenced and shown to possess a striking polymorphism that determines specific patterns of both activation and inhibition of the quorum-sensing response. We have developed a simple purification method for the modified peptide signalling pheromones allowing the characterization of four distinct ComX molecules representing different pherotypes. Genetic and biochemical evidence demonstrate that all the ComX variants are isoprenylated by the post-translational modification of a conserved tryptophan residue and that the modifications on the ComX peptide backbones vary in mass among the various pherotypes. These results give new insights into peptidemediated quorum-sensing signalling in Gram-positive bacteria and emphasize the role of isoprenylation in bacterial signal transduction. 相似文献
74.
Rijavec M Budic M Mrak P Müller-Premru M Podlesek Z Zgur-Bertok D 《Applied and environmental microbiology》2007,73(3):1029-1032
Colicin K exhibited pronounced inhibitory activity against uropathogenic Escherichia coli (UPEC) strains. Low prevalence of colicin K production and a relatively high prevalence of ColE1-like plasmids were determined among 215 UPEC strains from Slovenia. Sequencing of the colicin K-encoding pColK-K235 revealed a mosaic structure and the presence of the insertion sequence IS2. 相似文献
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Aleksandra Krivograd Klemenčič Nataša Smolar-Žvanut Darja Istenič Tjaša Griessler-Bulc 《Biologia》2010,65(3):422-437
In 2005 and 2006, epiphyton samples were collected from seven lowland and montane peat bogs in Slovenia. Water temperature,
pH, conductivity, dissolved oxygen and saturation were measured at the same time. Diatoms, desmids and Cyanobacteria were
the most abundand groups in species number. Canonical Correspondence Analysis (CCA) was carried out on Cyanobacteria, diatom
and desmid flora composition. This analysis showed that shading was the most important parameter in Cyanobacteria distribution
and bedrock the most important one in that of diatoms and desmids. Cluster analyses were carried out based on the Cyanobacteria,
diatom and desmid data. The Cyanobacteria and diatom data separated sites, whereas the desmid data revealed a temporal aspect. 相似文献
80.
Hostnik P Toplak I Barlic-Maganja D Grom J Bidovec A 《Journal of wildlife diseases》2006,42(2):459-465
Red foxes (Vulpes vulpes) are the main reservoir of rabies in Slovenia, whereas cases of rabies in other wildlife species occur sporadically. In 1995, a program of oral vaccination of wildlife in Slovenia was initiated; baits with oral vaccine were distributed by air at a density of 20 baits/km(2). During 1995, when the oral vaccination program was started, 1,089 cases of rabies (including both wild and domestic animals) were reported. Five years later (1999), only six positive animals were detected among 1,195 tested (0.5%). Despite an increase in bait density (25 baits/km(2)) during the years 2000 and 2001, reported rabies cases increased to 115 and 135, respectively. In 2003, following initiation of a new bait-dropping strategy, which incorporated perpendicular rather than parallel flight lines, the number of rabies cases decreased to eight. 相似文献