排序方式: 共有31条查询结果,搜索用时 156 毫秒
21.
A Kunche MA Kiresur A Ananthaneni VS Guduru HK Puneeth B Bagalad 《Biotechnic & histochemistry》2017,92(8):547-551
Tumors of the oral cavity include combinations of hard and soft tissues that may be difficult to identify using routine hematoxylin and eosin (H & E) staining. Although combination stains can demonstrate hard and soft tissues, trichrome stains, such as VanGieson and Masson, cannot differentiate dental hard tissues, such as dentin, cementum and osteoid. Modified Gallegos (MGS) and verdeluz orange G-acid fuchsin (VOF) stains can differentiate components of teeth. We used 10 tissue sections of decalcified bone and 10 pathologic tissue sections that contained different calcified tissues including peripheral ossifying fibroma, odontoma, central ossifying fibroma and cemento-ossifying fibroma. Sections were stained with H & E, VOF or MGS. H and E stained both hard tissues pink. VOF stained bone purple-red, cementum red and collagen blue. MGS stained bone green-blue, cementum red and collagen blue. VOF staining intensity and differentiation was better than MGS staining. VOF staining demonstrated hard tissue components distinctly and exhibited good contrast with the surrounding connective tissue. VOF also is a simple, single step, rapid staining procedure. 相似文献
22.
Juliana HK Kim John B Davidson Oliver Röhrle Tanya K Soboleva Andrew J Pullan 《Biomedical engineering online》2007,6(1):48
Background
Functional Electrical Stimulation (FES) is a technique that aims to rehabilitate or restore functionality of skeletal muscles using external electrical stimulation. Despite the success achieved within the field of FES, there are still a number of questions that remain unanswered. One way of providing input to the answers is through the use of computational models. 相似文献23.
The major metabolic route for the synthesis of phosphoenolpyruvate is from 2-phosphoglycerate catalyzed by the enzyme enolase (EC 4.2.1.11). Enolase occurs at the converging point between glycolysis and gluconeogenesis and may be an important regulatory enzyme. Growth ofEscherichia coli JA 200 pLC 11-8 to stationary phase in low-phosphate medium containing32P-orthophosphate and glucose as the carbon source resulted in incorporation of label into the enzyme. In vivo labeling of enolase was demonstrated by immunoaffinity chromatography of the labeled crude extract. In addition,32P-enolase was identified with sodium dodecylsulfate polyacrylamide gels, two-dimensional gel electrophoresis, and Western blot analysis, followed by autoradiography. 相似文献
24.
Zhenming Zhao Nadia Timofeev Stephen W Hartley David HK Chui Supan Fucharoen Thomas T Perls Martin H Steinberg Clinton T Baldwin Paola Sebastiani 《BMC genetics》2008,9(1):1-8
Background
Polymorphism in genes of regulating enzymes, transporters and receptors of the neurotransmitters of the central nervous system have been associated with altered behaviour, and single nucleotide polymorphisms (SNPs) represent the most frequent type of genetic variation. The serotonin and dopamine signalling systems have a central influence on different behavioural phenotypes, both of invertebrates and vertebrates, and this study was undertaken in order to explore genetic variation that may be associated with variation in behaviour.Results
Single nucleotide polymorphisms in canine genes related to behaviour were identified by individually sequencing eight dogs (Canis familiaris) of different breeds. Eighteen genes from the dopamine and the serotonin systems were screened, revealing 34 SNPs distributed in 14 of the 18 selected genes. A total of 24,895 bp coding sequence was sequenced yielding an average frequency of one SNP per 732 bp (1/732). A total of 11 non-synonymous SNPs (nsSNPs), which may be involved in alteration of protein function, were detected. Of these 11 nsSNPs, six resulted in a substitution of amino acid residue with concomitant change in structural parameters.Conclusion
We have identified a number of coding SNPs in behaviour-related genes, several of which change the amino acids of the proteins. Some of the canine SNPs exist in codons that are evolutionary conserved between five compared species, and predictions indicate that they may have a functional effect on the protein. The reported coding SNP frequency of the studied genes falls within the range of SNP frequencies reported earlier in the dog and other mammalian species. Novel SNPs are presented and the results show a significant genetic variation in expressed sequences in this group of genes. The results can contribute to an improved understanding of the genetics of behaviour. 相似文献25.
26.
27.
H. Kathleen Dannelly Jean-Claude Cortay Alain J. Cozzone Henry C. Reeves 《Current microbiology》1989,19(4):237-240
Enolase is involved in both glycolysis and gluconeogenesis, catalyzing the interconversion of 2-phosphoglycerate to phosphoenolpyruvate. InEscherichia coli, enolase has been shown previously to incorporate [32P] when cells were labeled in vivo; the phosphorylation has been shown to modulate the activity of the enzyme. Reported here is the phosphoamino acid analysis of in vivo-labeled enolase, which was shown to be phosphorylated on a serine residue(s). Also presented are results of studies demonstrating the localization of phosphoenolase on a two-dimensional map based on coordinates reported earlier, and a phosphoamino acid analysis of that protein, which finding corroborates the identification of phosphoserine in enolase. 相似文献
28.
29.
30.