排序方式: 共有128条查询结果,搜索用时 15 毫秒
101.
Danila Cuccu Marco Mereu Pamela Masala Angelo Cau Patrizia Jereb 《Invertebrate reproduction & development.》2013,57(1):16-21
The male reproductive system of the bobtail squid Neorossia caroli (Cephalopoda: Sepiolidae) is described in detail, based on observations of 90 mature males caught from 500 to 1600?m depth in Sardinian waters (western Mediterranean Sea). Reproductive organs in mature specimens accounted for up to 6% of total body weight. Of this, 70% was represented by the spermatophoric complex. Up to 83 spermatophores were found inside the Needham's sac. Mean spermatophore length was 16.7?mm. Sperm mass, cement body, and ejaculatory apparatus represented 63.2%, 13.1%, and 23.7% of the total spermatophore length, respectively. Inverted spermatophores, empty spermatophore sheaths, and spermatangia were also found in the sac, and their presence is discussed. Spermatangia implanted in several parts of the bodies of males (e.g., head, funnel, and eyes) were recorded and their occurrence is discussed. The spermatophoric reaction was induced in the laboratory by submerging spermatophores in seawater, and it is described briefly. 相似文献
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Flavia Agata Cimini Andrea Arena Ilaria Barchetta Antonella Tramutola Valentina Ceccarelli Chiara Lanzillotta Mario Fontana Laura Bertoccini Frida Leonetti Danila Capoccia Gianfranco Silecchia Claudio Di Cristofano Caterina Chiappetta Fabio Di Domenico Marco Giorgio Baroni Marzia Perluigi Maria Gisella Cavallo Eugenio Barone 《生物化学与生物物理学报:疾病的分子基础》2019,1865(6):1490-1501
Biliverdin reductase-A (BVR-A) is a serine/threonine/tyrosine kinase involved in the regulation of insulin signaling. In vitro studies have demonstrated that BVR-A is a substrate of the insulin receptor and regulates IRS1 by avoiding its aberrant activation, and in animal model of obesity the loss of hepatic BVR-A has been associated with glucose/insulin alterations and fatty liver disease. However, no studies exist in humans. Here, we evaluated BVR-A expression levels and activation in peripheral blood mononuclear cells (PBMC) from obese subjects and matched lean controls and we investigated the related molecular alterations of the insulin along with clinical correlates. We showed that BVR-A levels are significantly reduced in obese subjects and associated with a hyper-activation of the IR/IRS1/Akt/GSK-3β/AS160/GLUT4 pathway. Low BVR-A levels also associate with the presence of obesity, metabolic syndrome, NASH and visceral adipose tissue inflammation. These data suggest that the reduction of BVR-A may be responsible for early alterations of the insulin signaling pathway in obesity and in this context may represent a novel molecular target to be investigated for the comprehension of the process of insulin resistance development in obesity. 相似文献
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James M. Kelley Laura B. Hughes Jeffrey D. Faggard Maria I. Danila Monica H. Crawford Yuanqing Edberg Miguel A. Padilla Hemant K. Tiwari Andrew O. Westfall Graciela S. Alarcn Doyt L. Conn Beth L. Jonas Leigh F. Callahan Edwin A. Smith Richard D. Brasington Jr David B. Allison Robert P. Kimberly Larry W. Moreland Jeffrey C. Edberg S. Louis Bridges Jr 《PLoS genetics》2009,5(3)
Cytotoxic T-lymphocyte associated protein 4 (CTLA4) is a negative regulator of T-cell proliferation. Polymorphisms in CTLA4 have been inconsistently associated with susceptibility to rheumatoid arthritis (RA) in populations of European ancestry but have not been examined in African Americans. The prevalence of RA in most populations of European and Asian ancestry is ~1.0%; RA is purportedly less common in black Africans, with little known about its prevalence in African Americans. We sought to determine if CTLA4 polymorphisms are associated with RA in African Americans. We performed a 2-stage analysis of 12 haplotype tagging single nucleotide polymorphisms (SNPs) across CTLA4 in a total of 505 African American RA patients and 712 African American controls using Illumina and TaqMan platforms. The minor allele (G) of the rs231778 SNP was 0.054 in RA patients, compared to 0.209 in controls (4.462×10−26, Fisher's exact). The presence of the G allele was associated with a substantially reduced odds ratio (OR) of having RA (AG+GG genotypes vs. AA genotype, OR 0.19, 95% CI: 0.13–0.26, p=2.4×10−28, Fisher's exact), suggesting a protective effect. This SNP is polymorphic in the African population (minor allele frequency [MAF] 0.09 in the Yoruba population), but is very rare in other groups (MAF=0.002 in 530 Caucasians genotyped for this study). Markers associated with RA in populations of European ancestry (rs3087243 [+60C/T] and rs231775 [+49A/G]) were not replicated in African Americans. We found no confounding of association for rs231778 after stratifying for the HLA-DRB1 shared epitope, presence of anti-cyclic citrullinated peptide antibody, or degree of admixture from the European population. An African ancestry-specific genetic variant of CTLA4 appears to be associated with protection from RA in African Americans. This finding may explain, in part, the relatively low prevalence of RA in black African populations. 相似文献
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There is a growing need to develop novel therapeutics for targeted treatment of cancer. The prerequisite to success is the knowledge about which types of molecular alterations are predominantly driving tumorigenesis. To shed light onto this subject, we have utilized the largest database of human cancer mutations–TCGA PanCanAtlas, multiple established algorithms for cancer driver prediction (2020plus, CHASMplus, CompositeDriver, dNdScv, DriverNet, HotMAPS, OncodriveCLUSTL, OncodriveFML) and developed four novel computational pipelines: SNADRIF (Single Nucleotide Alteration DRIver Finder), GECNAV (Gene Expression-based Copy Number Alteration Validator), ANDRIF (ANeuploidy DRIver Finder) and PALDRIC (PAtient-Level DRIver Classifier). A unified workflow integrating all these pipelines, algorithms and datasets at cohort and patient levels was created. We have found that there are on average 12 driver events per tumour, of which 0.6 are single nucleotide alterations (SNAs) in oncogenes, 1.5 are amplifications of oncogenes, 1.2 are SNAs in tumour suppressors, 2.1 are deletions of tumour suppressors, 1.5 are driver chromosome losses, 1 is a driver chromosome gain, 2 are driver chromosome arm losses, and 1.5 are driver chromosome arm gains. The average number of driver events per tumour increases with age (from 7 to 15) and cancer stage (from 10 to 15) and varies strongly between cancer types (from 1 to 24). Patients with 1 and 7 driver events per tumour are the most frequent, and there are very few patients with more than 40 events. In tumours having only one driver event, this event is most often an SNA in an oncogene. However, with increasing number of driver events per tumour, the contribution of SNAs decreases, whereas the contribution of copy-number alterations and aneuploidy events increases. 相似文献
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Natalia I Minaeva Evgeny R Gak Danila V Zimenkov Aleksandra Yu Skorokhodova Irina V Biryukova Sergey V Mashko 《BMC biotechnology》2008,8(1):63
Background
The development of modern producer strains with metabolically engineered pathways poses special problems that often require manipulating many genes and expressing them individually at different levels or under separate regulatory controls. The construction of plasmid-less marker-less strains has many advantages for the further practical exploitation of these bacteria in industry. Such producer strains are usually constructed by sequential chromosome modifications including deletions and integration of genetic material. For these purposes complex methods based on in vitro and in vivo recombination processes have been developed. 相似文献108.
Maria Cristina Follesa Danila Cuccu Rita Cannas Serenella Cabiddu Matteo Murenu Andrea Sabatini Angelo Cau 《Hydrobiologia》2008,606(1):63-68
The contribution that a small restocking area (central western Mediterranean) has made to the Palinurus elephas (Fabr., 1787) population was examined by comparing the abundances recorded inside and outside the area before (1997) and
after the establishment of the reserve (1998–2005). From 1998 to 2002 a progressive percentage increase of P. elephas biomass values was recorded both inside the area and in the surrounding zones. The total mean abundance within the reserve
(CPUE = 0.23 ± 0.10 kg/50 m/boat) was 7.5 times greater than that for the neighbouring zone (CPUE = 0.03 ± 0.07 kg/50 m/boat).
The inter-annual analysis of lobster size inside the area also showed a progressive increase of adults and juveniles. The
results highlighted the effectiveness of fishing restrictions in rebuilding the lobsters population and suggest that small
MPAs should be set up.
Guest editors: J. Davenport, G. Burnell, T. Cross, M. Emmerson, R. McAllen, R. Ramsay & E. Rogan
Challenges to Marine Ecosystems 相似文献
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Sheehy JE Gunawardana D Ferrer AB Danila F Tan KG Mitchell PL 《The New phytologist》2008,179(3):579-582