Ethnobotany of Horseradish (<Emphasis Type="Italic">Armoracia rusticana</Emphasis>, Brassicaceae) and Its Wild Relatives (<Emphasis Type="Italic">Armoracia</Emphasis> spp.): Reproductive Biology and Local Uses in Their Native Ranges

Ethnobotany of Horseradish ( Armoracia rusticana , Brassicaceae) and Its Wild Relatives ( Armoracia spp.): Reproductive Biology and Local Uses in Their Native Ranges. Horseradish (Armoracia rusticana) has been cultivated for its root for over 2,000 years. Today, commercial production takes place primarily in North America and Europe where A. rusticana is propagated exclusively vegetatively. Like many vegetatively-propagated crops, cultivated A. rusticana plants are generally sterile. Armoracia rusticana is one of three species in the genus: A. macrocarpa is native to the Central Danube River Basin, A. rusticana is putatively native to Eastern Europe, and A. sisymbrioides is native to Siberia. Documenting the ways in which the three Armoracia species are used in their putative native ranges and the reproductive biology of Armoracia in these regions will advance understanding of the role of humans in the evolution of sterility in A. rusticana. In this broad-scale ethnobotanical analysis, we document 1) which Armoracia species are used in different geographic regions, 2) the reproductive biology of cultivated and natural Armoracia populations, and 3) the uses of Armoracia species in their native areas. Fieldwork was conducted in Austria, Bulgaria, Romania, and Russia. Ethnobotanical data were collected through written questionnaires. In all areas visited, A. rusticana is the primary Armoracia species; however, there is regional variation in the way the plant is used. Armoracia rusticana is propagated vegetatively and is not found in the wild. Armoracia macrocarpa and A. sisymbrioides reproduce by seed in nature. These data provide an understanding of contemporary uses and management strategies of Armoracia species in their native ranges, demonstrate the differential reproductive biology of a clonally-propagated crop relative to its wild progenitors, and emphasize the importance of ethnobotanical data collection for crops and their wild relatives from a broad geographic range.     

Analysis of multiparasitism by Eupelmus vuilleti (Craw) (Eupelmidae) and Dinarmus basalis (Rond) (Pteromalidae) in the presence of one of their common hosts,Bruchidius atrolineatus (Pic) (Coleoptera Bruchidae)

Bruchidius atrolineatus (Pic) is a tropical beetle (Coleoptera Bruchidae) that develops during the larval and pupal stages in the seeds of a legume Vigna unguiculata (Walp). Two species of Hymenoptera, Dinarmus basalis (Rond) and Eupelmus vuilleti (Craw), solitary ectoparasitoids of the larvae and pupae of B. atrolineatus, were introduced successively in the presence of their hosts, varying the interval between the two introductions. When D. basalis females were introduced 24 h, 3 days or 7 days after E. vuilleti, multiparasitism was low. The females had low fecundity, and their eggs were not distributed randomly over the different available hosts. When E. vuilleti females were introduced second, they oviposited on the different hosts availabe and did not avoid multiparasitism. The presence of hosts already parasitised by D. basalis increased the reproduction of E. vuilleti, and the fecundity of the females was higher than in control batches with E. vuilleti alone. E. vuilleti seems capable of detecting the ovipositor shafts drilled by the D. basalis females, and by introducing its own ovipositors killing the D. basalis eggs or larvae. When interspecific competition was occurring the number of E. vuilleti adults emerging from the seeds was no different from that observed in control batches with E. vuilleti alone, and there were always fewer D. basalis adults than in control batches (D. basalis alone). This interspecific competition reduces the influence of the two parasitoids in the biological control of bruchid populations.     

Cloning and nucleotide sequence of the bglA gene from Erwinia herbicola and expression of β-glucosidase activity in Escherichia coli

Abstract Genomic DNA fragments encoding β-glucosidase activity from the wild-type strain WD4 of Erwinia herbicola were cloned into Escherichia coli . Two clones containing a common fragment encoded a polypeptide of 58000 Da. Cloned β-glucosidase, expressed in E. coli , showed activity against natural β-glucoside sugars except for cellobiose. An open reading frame of 1442 bp termed bglA was identified by nucleotide sequencing and it coded for a protein of 480 amino acids ( M _r 53896) which showed significant homology with β-glucosidases from glycosyl hydrolase family 1.     

Axoplasmic flow of adrenaline and monoamine oxidase in amphibian sympathetic nerves

Summary The accumulation of both A and MAO proximal to a ligature on toad spinal nerves has been shown to occur at a slower rate than in mammals. As in mammals, there are two components of axonal transport in amphibian nerves, with the accumulation of A reaching a peak at between 4 and 7 days (cf. 2–4 days for NA in mammals), while MAO accumulation does not reach its maximum before 9 days (cf. 7 days in mammals). No accumulation occurs after sympathectomy, providing evidence for localization of MAO within amphibian sympathetic adrenergic nerves. Distal accumulation of MAO occurs in toad sympathetic nerves; this has not been reported to occur in mammalian nerves. Distal accumulation reaches a peak at 2–4 days, which suggests either a fast retrograde flow of MAO or that induction of MAO is occurring. These results are discussed in relation to differences between mammalian and amphibian sympathetic nerves and to the events occurring following ligation of these nerves.We wish to thank Judy Lenk, Vivienne Einhorn and Barbara Peachey for their assistance with the initial MAO histochemical work. This work was supported by grants from the National Heart Foundation of Australia and the Australian Research Grants Committee.