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991.
Size‐based interactions and trophic transfer efficiency are modified by fish predation and cyanobacteria blooms in Lake Mývatn,Iceland
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Zeynep Ersoy Erik Jeppesen Serena Sgarzi Ignasi Arranz Miguel Cañedo‐Argüelles Xavier D. Quintana Frank Landkildehus Torben L. Lauridsen Mireia Bartrons Sandra Brucet 《Freshwater Biology》2017,62(11):1942-1952
992.
Trimethylamine N-oxide (TMAO) is a naturally occurring osmolyte that stabilizes proteins, induces folding, and counteracts the denaturing effects of urea, pressure, and ice. To establish the mechanism behind these effects, isotopic substitution neutron-scattering measurements were performed on aqueous solutions of TMAO and 1:1 TMAO-urea at a solute mole fraction of 0.05. The partial pair distribution functions were extracted using the empirical potential structure refinement method. The results were compared with previous results obtained with isosteric tert-butanol, as well as the available data from spectroscopy and molecular-dynamics simulations. In solution, the oxygen atom of TMAO is strongly hydrogen-bonded to, on average, between two and three water molecules, and the hydrogen-bond network is tighter in water than in pure water. In TMAO-urea solutions, the oxygen atom in TMAO preferentially forms hydrogen bonds with urea. This explains why the counteraction is completed at a 2:1 urea/TMAO concentration ratio, independently of urea concentration. These results strongly support models for the effect of TMAO on the stability of proteins based on a modification of the simultaneous equilibria that control hydrogen bonding between the peptide backbone and water or intramolecular sites, without any need for direct interaction between TMAO and the protein. 相似文献
993.
994.
Biodegradation of a crude oil by three microbial consortia of different origins and metabolic capabilities 总被引:6,自引:0,他引:6
Viñas M Grifoll M Sabaté J Solanas AM 《Journal of industrial microbiology & biotechnology》2002,28(5):252-260
Microbial consortia were obtained three by sequential enrichment using different oil products. Consortium F1AA was obtained
on a heavily saturated fraction of a degraded crude oil; consortium TD, by enrichment on diesel and consortium AM, on a mixture
of five polycyclic aromatic hydrocarbons [PAHs]. The three consortia were incubated with a crude oil in order to elucidate
their metabolic capabilities and to investigate possible differences in the biodegradation of these complex hydrocarbon mixtures
in relation to their origin. The efficiency of the three consortia in removing the saturated fraction was 60% (F1AA), 48%
(TD) and 34% (AM), depending on the carbon sources used in the enrichment procedures. Consortia F1AA and TD removed 100% of
n-alkanes and branched alkanes, whereas with consortium AM, 91% of branched alkanes remained. Efficiency on the polyaromatic
fraction was 19% (AM), 11% (TD) and 7% (F1AA). The increase in aromaticity of the polyaromatic fraction during degradation
of the crude oil by consortium F1AA suggested that this consortium metabolized the aromatic compounds primarily by oxidation
of the alkylic chains. The 500-fold amplification of the inocula from the consortia by subculturing in rich media, necessary
for use of the consortia in bioremediation experiments, showed no significant decrease in their degradation capability. Journal of Industrial Microbiology & Biotechnology (2002) 28, 252–260 DOI: 10.1038/sj/jim/7000236
Received 12 July 2001/ Accepted in revised form 11 November 2001 相似文献
995.
Genetic and morphological differentiation between the two largest breeding colonies of Audouin's Gull Larus audouinii 总被引:1,自引:0,他引:1
We assessed the genetic and morphological differences between the two largest breeding colonies of Audouin's Gull Larus audouinii , an endemic seabird species of the Mediterranean region. The two colonies comprise c. 75% of the total world population and are 655 km apart. The Ebro Delta colony was formed recently and, after dramatic growth mainly due to high rates of both immigration and reproductive success, is now the largest in the world (more than 60% of the total population). The Chafarinas Islands support an ancient colony with relatively little fluctuation in breeding numbers. The two colonies also differ greatly in environmental conditions, with the Ebro Delta being a higher quality breeding site. Very little movement occurs between the two colonies. We collected morphological data and blood samples from both colonies. Polymorphic microsatellite markers were used to study the genetic differentiation. These showed no significant variation between colonies, nor evidence of a founder effect in the Ebro Delta. Individuals from the Ebro Delta were larger than those from Chafarinas, the difference being greater for males. This probably reflects a stronger male susceptibility to worse environmental conditions during chick growth at the Chafarinas Islands. 相似文献
996.
Iñigo Zuberogoitia Jabi Zabala Jon Etxezarreta Ariñe Crespo Gorka Burgos Juan Arizaga 《Population Ecology》2016,58(2):303-313
Climate change affects the climatic disturbance patterns and regimes and is altering the frequency and intensity of subtropical cyclones. These events can affect population dynamics of seabirds (e.g., survival, reproduction). In this work we tested the effect of adverse weather on a colony of European storm petrels (Hydrobates pelagicus) located in a small islet (Aketx) in northern Spain. Over a long-term monitoring period (1993–2014) we ringed 3728 petrels. From 2003 onwards we also monitored breeding success, the percentage of immature individuals and moult scores. We used Cormack-Jolly-Seber models and Underhill and Zucchini models to analyze the effects of climatic conditions on a number of biological traits (survival, breeding parameters, moulting patterns). Our analyses revealed a constant value of adult survival over the 26-year monitoring period. Recapture probability, however, tended to be positively influenced by NAO conditions in spring, and negatively influenced by NAO conditions in winter (although this would only affect to a fraction of first-captured birds). Moreover, the impact of adverse weather, especially in 2011 and 2014, resulted in an increasing proportion of yearlings in the breeding population, a lower breeding success and a delayed onset of moult. These effects were similar to those observed during the Prestige oil spill catastrophe. 相似文献
997.
Replication study of 34 common SNPs associated with prostate cancer in the Romanian population
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Viorel Jinga Irma Eva Csiki Andrei Manolescu Paul Iordache Ioan Nicolae Mates Daniel Radavoi Stefan Rascu Daniel Badescu Paula Badea Dana Mates 《Journal of cellular and molecular medicine》2016,20(4):594-600
Prostate cancer is the third‐most common form of cancer in men in Romania. The Romanian unscreened population represents a good sample to study common genetic risk variants. However, a comprehensive analysis has not been conducted yet. Here, we report our replication efforts in a Romanian population of 979 cases and 1027 controls, for potential association of 34 literature‐reported single nucleotide polymorphisms (SNPs) with prostate cancer. We also examined whether any SNP was differentially associated with tumour grade or stage at diagnosis, with disease aggressiveness, and with the levels of PSA (prostate specific antigen). In the allelic analysis, we replicated the previously reported risk for 19 loci on 4q24, 6q25.3, 7p15.2, 8q24.21, 10q11.23, 10q26.13, 11p15.5, 11q13.2, 11q13.3. Statistically significant associations were replicated for other six SNPs only with a particular disease phenotype: low‐grade tumour and low PSA levels (rs1512268), high PSA levels (rs401681 and rs11649743), less aggressive cancers (rs1465618, rs721048, rs17021918). The strongest association of our tested SNP's with PSA in controls was for rs2735839, with 29% increase for each copy of the major allele G, consistent with previous results. Our results suggest that rs4962416, previously associated only with prostate cancer, is also associated with PSA levels, with 12% increase for each copy of the minor allele C. The study enabled the replication of the effect for the majority of previously reported genetic variants in a set of clinically relevant prostate cancers. This is the first replication study on these loci, known to associate with prostate cancer, in a Romanian population. 相似文献
998.
Annelene Pengerud Marie-France Dignac Giacomo Certini Line Tau Strand Claudia Forte Daniel P. Rasse 《Biogeochemistry》2017,135(3):277-292
Increased mineralization of the organic matter (OM) stored in permafrost is expected to constitute the largest additional global warming potential from terrestrial ecosystems exposed to a warmer climate. Chemical composition of permafrost OM is thought to be a key factor controlling the sensitivity of decomposition to warming. Our objective was to characterise OM from permafrost soils of the European Arctic: two mineral soils—Adventdalen, Svalbard, Norway and Vorkuta, northwest Russia—and a “palsa” (ice-cored peat mound patterning in heterogeneous permafrost landscapes) soil in Neiden, northern Norway, in terms of molecular composition and state of decomposition. At all sites, the OM stored in the permafrost was at an advanced stage of decomposition, although somewhat less so in the palsa peat. By comparing permafrost and active layers, we found no consistent effect of depth or permafrost on soil organic matter (SOM) chemistry across sites. The permafrost-affected palsa peat displayed better preservation of plant material in the deeper layer, as indicated by increasing contribution of lignin carbon to total carbon with depth, associated to decreasing acid (Ac) to aldehyde (Al) ratio of the syringyl (S) and vanillyl (V) units, and increasing S/V and contribution of plant-derived sugars. By contrast, in Adventdalen, the Ac/Al ratio of lignin and the Alkyl C to O-alkyl C ratio in the NMR spectra increased with depth, which suggests less oxidized SOM in the active layer compared to the permafrost layer. In Vorkuta, SOM characteristics in the permafrost profile did not change substantially with depth, probably due to mixing of soil layers by cryoturbation. The composition and state of decomposition of SOM appeared to be site-specific, in particular bound to the prevailing organic or mineral nature of soil when attempting to predict the SOM proneness to degradation. The occurrence of processes such as palsa formation in organic soils and cryoturbation should be considered when up-scaling and predicting the responses of OM to climate change in arctic soils. 相似文献
999.
The type of competition modulates the ecophysiological response of grassland species to elevated CO2 and drought
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J. Miranda‐Apodaca U. Pérez‐López M. Lacuesta A. Mena‐Petite A. Muñoz‐Rueda 《Plant biology (Stuttgart, Germany)》2015,17(2):298-310
The effects of elevated CO2 and drought on ecophysiological parameters in grassland species have been examined, but few studies have investigated the effect of competition on those parameters under climate change conditions. The objective of this study was to determine the effect of elevated CO2 and drought on the response of plant water relations, gas exchange, chlorophyll a fluorescence and aboveground biomass in four grassland species, as well as to assess whether the type of competition modulates that response. Elevated CO2 in well‐watered conditions increased aboveground biomass by augmenting CO2 assimilation. Drought reduced biomass by reducing CO2 assimilation rate via stomatal limitation and, when drought was more severe, also non‐stomatal limitation. When plants were grown under the combined conditions of elevated CO2 and drought, drought limitation observed under ambient CO2 was reduced, permitting higher CO2 assimilation and consequently reducing the observed decrease in aboveground biomass. The response to climate change was species‐specific and dependent on the type of competition. Thus, the response to elevated CO2 in well‐watered grasses was higher in monoculture than in mixture, while it was higher in mixture compared to monoculture for forbs. On the other hand, forbs were more affected than grasses by drought in monoculture, while in mixture the negative effect of drought was higher in grasses than in forbs, due to a lower capacity to acquire water and mineral nutrients. These differences in species‐level growth responses to CO2 and drought may lead to changes in the composition and biodiversity of the grassland plant community in future climate conditions. 相似文献
1000.
Identification of CD46 binding sites within the adenovirus serotype 35 fiber knob 总被引:2,自引:1,他引:2
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Wang H Liaw YC Stone D Kalyuzhniy O Amiraslanov I Tuve S Verlinde CL Shayakhmetov D Stehle T Roffler S Lieber A 《Journal of virology》2007,81(23):12785-12792
Species B human adenoviruses (Ads) are often associated with fatal illnesses in immunocompromised individuals. Recently, species B Ads, most of which use the ubiquitously expressed complement regulatory protein CD46 as a primary attachment receptor, have gained interest for use as gene therapy vectors. In this study, we focused on species B Ad serotype 35 (Ad35), whose trimeric fiber knob domain binds to three CD46 molecules with a KD (equilibrium dissociation constant) of 15.5 nM. To study the Ad35 knob-CD46 interaction, we generated an expression library of Ad35 knobs with random mutations and screened it for CD46 binding. We identified four critical residues (Phe242, Arg279, Ser282, and Glu302) which, when mutated, ablated Ad35 knob binding to CD46 without affecting knob trimerization. The functional importance of the identified residues was validated in surface plasmon resonance and competition binding studies. To model the Ad35 knob-CD46 interaction, we resolved the Ad35 knob structure at 2-Å resolution by X-ray crystallography and overlaid it onto the existing structure for Ad11-CD46 interaction. According to our model, all identified Ad35 residues are in regions that interact with CD46, whereby one CD46 molecule binds between two knob monomers. This mode of interaction might have potential consequences for CD46 signaling and intracellular trafficking of Ad35. Our findings are also fundamental for better characterization of species B Ads and design of antiviral drugs, as well as for application of species B Ads as in vivo and in vitro gene transfer vectors. 相似文献