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191.
Anna Kletzmayr Flurina Clement Frey Miriam Zimmermann Daniel Eberli Christopher Millan 《Biotechnology journal》2020,15(5)
The microenvironment plays a major role in conferring chemoresistance to cancer cells. In order to better inform clinical response to chemoresistance, preclinical models that recapitulate its hallmark features are needed to enable screening for resistance‐specific therapeutic targets. A novel platform for seeding cancer cells in 3D hydrogels is presented utilizing derivatives of chitosan and alginate that, critically, is amenable to high throughput screening: cell seeding in hydrogels, media changes, dosing of anticancer compounds, and cell viability assays are all automated using a standard and commercially available liquid handling robot. Culture in these hydrogels elicits resistance in ovarian, lung, and prostate cancer cells to treatment by doxorubicin and paclitaxel. In correlation, proteomics analysis of SKOV3 cells cultured in 3D reveals enrichment of proteins associated with extreme drug resistance including HMOX1 and ALDH2. Subsequently, therapeutic antibodies targeted to tumor‐associated antigens upregulated in 3D cultures are shown to have higher efficacy compared to 2D cultures. Collectively, this automated 3D cell culture platform provides a powerful tool with utility in identification of drugs that may overcome chemoresistance. 相似文献
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Maurer Daniel Malique François Alfarraj Salah Albasher Gada Horn Marcus A. Butterbach-Bahl Klaus Dannenmann Michael Rennenberg Heinz 《Plant and Soil》2021,461(1-2):107-118
Plant and Soil - Cyperaceae are common on nutrient-poor soils, including in western Japan. We examined the ability of the native Cyperaceae from western Japan to form dauciform roots and assessed... 相似文献
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Neurons exhibit diverse intrinsic dynamics, which govern how they integrate synaptic inputs to produce spikes. Intrinsic dynamics are often plastic during development and learning, but the effects of these changes on stimulus encoding properties are not well known. To examine this relationship, we simulated auditory responses to zebra finch song using a linear-dynamical cascade model, which combines a linear spectrotemporal receptive field with a dynamical, conductance-based neuron model, then used generalized linear models to estimate encoding properties from the resulting spike trains. We focused on the effects of a low-threshold potassium current (KLT) that is present in a subset of cells in the zebra finch caudal mesopallium and is affected by early auditory experience. We found that KLT affects both spike adaptation and the temporal filtering properties of the receptive field. The direction of the effects depended on the temporal modulation tuning of the linear (input) stage of the cascade model, indicating a strongly nonlinear relationship. These results suggest that small changes in intrinsic dynamics in tandem with differences in synaptic connectivity can have dramatic effects on the tuning of auditory neurons. 相似文献
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O’Byrne Alice M. Lambourn Dyanna M. Rejmanek Daniel Haman Katherine O’Byrne Michael VanWormer Elizabeth Shapiro Karen 《EcoHealth》2021,18(1):84-94
EcoHealth - Increasing reports of marine mammal deaths have been attributed to the parasite Sarcocystis neurona. Infected opossums, the only known definitive hosts, shed S. neurona sporocysts in... 相似文献
200.
Whitfield Alan K. Adams Janine B. Harrison Trevor D. Lamberth Stephen J. Lemley Daniel A. MacKay Fiona Van Niekerk Lara Weyl Olaf L. F. 《Biological invasions》2021,23(9):2729-2747
Biological Invasions - We review the possible impacts of non-native biota on the indigenous fishes of South African estuaries, including macrophytes, algae, pathogens, invertebrates, and fishes.... 相似文献