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101.
A winter bloom dominated by Pseudo-nitzschia calliantha Lundholm, Moestrup et Hasle (Bacillariophyceae), a potential domoic acid producer, is reported for the first time in the Aegean Sea, Greece, in a semi-enclosed embayment (Kalloni Gulf) surrounded by agricultural land and drained by intermittent rivers. Abundances of this species in the inner part of the Gulf during February were extremely high (max 1.1 × 107 cells l−1). The species Alexandrium insuetum Balech (Dinophyceae) was also found in considerable cell numbers (max 1.4 × 105 cells l−1) during the bloom and reached up to 40% of the total biovolume. This study demonstrates an evident cause and effect relationship between nutrient inflows originating from agricultural activities in the watershed and the development of a potential HAB. The massive bloom formation was observed soon after an episodic rainfall event during the fertilizer application period (December to February). A bloom was also observed the following year, but it was less pronounced due to the fact that rainfalls were more evenly spaced in time and were of moderate intensity. 相似文献
102.
Christelle Hély Kelly K. Caylor Peter Dowty Samuel Alleaume Robert J. Swap Hank H. Shugart Christopher O. Justice 《Ecosystems》2007,10(7):1116-1132
Abstract
We present a regional fuel load model (1 km2 spatial resolution) applied in the southern African savanna region. The model is based on a patch-scale production efficiency
model (PEM) scaled up to the regional level using empirical relationships between patch-scale behavior and multi-source remote
sensing data (spatio-temporal variability of vegetation and climatic variables). The model requires the spatial distribution
of woody vegetation cover, which is used to determine separate respiration rates for tree and grass. Net primary production,
grass and tree leaf death, and herbivory are also taken into account in this mechanistic modeling approach. The fuel load
model has been calibrated and validated from independent measurements taken from savanna vegetation in Africa southward from
the equator. A sensitivity analysis on the effect of climate variables (incoming radiation, air temperature, and precipitation)
has been conducted to demonstrate the strong role that water availability has in determining productivity and subsequent fuel
load over the southern African region. The model performance has been tested in four different areas representative of a regional
increasing rainfall gradient—Etosha National Park, Namibia, Mongu and Kasama, Zambia, as well as in Kruger National Park,
South Africa. Within each area, we analyze model output from three different magnitudes of canopy coverage (<5, 30, and 50%).
We find that fuel load ranges predicted by the model are globally in agreement with field measurements for the same year.
High rainfall sustains green herbaceous production late in the dry season and delays tree leaf litter production. Effect of
water on production varies across the rainfall gradient with delayed start of green material production in more arid regions. 相似文献
103.
104.
105.
Melissa A. Partridge Sumana Gopinath Simon J. Myers Jens R Coorssen 《Journal of chemical biology》2016,9(1):9-18
An initial proteomic analysis of the cuprizone mouse model to characterise the breadth of toxicity by assessing cortex, skeletal muscle, spleen and peripheral blood mononuclear cells. Cuprizone treated vs. control mice for an initial characterisation. Select tissues from each group were pooled, analysed in triplicate using two-dimensional gel electrophoresis (2DE) and deep imaging and altered protein species identified using liquid chromatography tandem mass spectrometry (LC/MS/MS). Forty-three proteins were found to be uniquely detectable or undetectable in the cuprizone treatment group across the tissues analysed. Protein species identified in the cortex may potentially be linked to axonal damage in this model, and those in the spleen and peripheral blood mononuclear cells to the minimal peripheral immune cell infiltration into the central nervous system during cuprizone mediated demyelination. Primary oligodendrocytosis has been observed in type III lesions in multiple sclerosis. However, the underlying mechanisms are poorly understood. Cuprizone treatment results in oligodendrocyte apoptosis and secondary demyelination. This initial analysis identified proteins likely related to axonal damage; these may link primary oligodendrocytosis and secondary axonal damage. Furthermore, this appears to be the first study of the cuprizone model to also identify alterations in the proteomes of skeletal muscle, spleen and peripheral blood mononuclear cells. Notably, protein disulphide isomerase was not detected in the cuprizone cohort; its absence has been linked to reduced major histocompatibility class I assembly and reduced antigen presentation. Overall, the results suggest that, like experimental autoimmune encephalomyelitis, results from the standard cuprizone model should be carefully considered relative to clinical multiple sclerosis. 相似文献
106.
Tapas K. Nandi Hridoy R. Bairagya Bishnu P. Mukhopadhyay K. Sekar Dipankar Sukul Asim K. Bera 《Journal of biosciences》2009,34(1):27-34
The role of invariant water molecules in the activity of plant cysteine protease is ubiquitous in nature. On analysing the
11 different Protein DataBank (PDB) structures of plant thiol proteases, the two invariant water molecules W1 and W2 (W220
and W222 in the template 1PPN structure) were observed to form H-bonds with the Ob atom of Asn 175. Extensive energy minimization and molecular dynamics simulation studies up to 2 ns on all the PDB and solvated
structures clearly revealed the involvement of the H-bonding association of the two water molecules in fixing the orientation
of the asparagine residue of the catalytic triad. From this study, it is suggested that H-bonding of the water molecule at
the W1 invariant site better stabilizes the Asn residue at the active site of the catalytic triad. 相似文献
107.
Keith G. Danielson Janice E. Knepper Frances S. Kittrell Janet S. Butel Daniel Medina Elisa M. Durban 《In vitro cellular & developmental biology. Plant》1989,25(6):535-543
Summary Clonal populations were isolated from the mouse mammary cell line, COMMA-D, by transfection with a dominant-selectable gene,
pSV2Neo, which confers resistance to the antibiotic, G418. Seven of twenty-four clones isolated retained the ability of the
parental line to repopulate cleared mammary fat pads in vivo as ductal-alveolar hyperplasias. Two sublines designated CDNR2
and CDNR4 retained hyperplastic growth potential after multiple passages in vitro with low incidence of tumor formation. A
third subpopulation, CDNR1, contained a single integration site for the pSV2Neo plasmid indicating a bonafide clonal origin
for this subline. CDNR1 cells displayed heterogeneous growth phenotypes in vivo including hyperplasia, adenocarcinoma, and
bone formation. Functional differentiation of CDNR1 cells organized as alveolarlike structures in vivo or on floating collagen
gels in vitro was observed as determined by immunoperoxidase staining for the milk-specific protein, casein. Overall, the
results indicate that a subset of cells from the COMMA-D cell line may be functionally analogous to stem cells existing in
the mammary gland.
Supported by NCI research grants CA-38650, CA-33369, CA-39017, and CA-25215. 相似文献
108.
Daniel A. Vardy Csaba Kari Gerald S. Lazarus Pamela J. Jensen Asher Zilberstein Gregory D. Plowman Ulrich Rodeck 《Journal of cellular physiology》1995,163(2):257-265
Autocrine activation of the epidermal growth factor (EGF) receptor on keratinocytes has been recognized as an important growth regulatory mechanism involved in epithelial homeostasis, and, possibly, hyperproliferative diseases. Insulin-like growth factor (IGF)-1 and insulin have been shown to be paracrine keratinocyte mitogens that bind to the type I IGF receptor which is expressed on actively proliferating keratinocytes in situ. In this report, we demonstrate that IGF-1/insulin induced production of keratinocyte-derived autocrine growth factors that bind to the EGF receptor. Increased steady-state mRNA levels for transforming growth factor alpha (TGF-α) and for amphiregulin (AR) were observed upon incubation of keratinocytes with mitogenic concentrations of IGF-1. IGF-1 also induced production and secretion of TGF-α and AR proteins as detected by immunoassays. An EGF receptor antagonistic monoclonal antibody abolished the mitogenic effect of IGF-1 on cultured keratinocytes. These results suggest that stimulation of keratinocyte growth by IGF-1 requires activation of an EGF receptor-mediated autocrine loop. © 1995 Wiley-Liss, Inc. 相似文献
109.
110.
Kathryn L. Butler Vijayakrishnan Ambravaneswaran Nitin Agrawal Maryelizabeth Bilodeau Mehmet Toner Ronald G. Tompkins Shawn Fagan Daniel Irimia 《PloS one》2010,5(7)
Thermal injury triggers a fulminant inflammatory cascade that heralds shock, end-organ failure, and ultimately sepsis and death. Emerging evidence points to a critical role for the innate immune system, and several studies had documented concurrent impairment in neutrophil chemotaxis with these post-burn inflammatory changes. While a few studies suggest that a link between neutrophil motility and patient mortality might exist, so far, cumbersome assays have prohibited exploration of the prognostic and diagnostic significance of chemotaxis after burn injury. To address this need, we developed a microfluidic device that is simple to operate and allows for precise and robust measurements of chemotaxis speed and persistence characteristics at single-cell resolution. Using this assay, we established a reference set of migration speed values for neutrophils from healthy subjects. Comparisons with samples from burn patients revealed impaired directional migration speed starting as early as 24 hours after burn injury, reaching a minimum at 72–120 hours, correlated to the size of the burn injury and potentially serving as an early indicator for concurrent infections. Further characterization of neutrophil chemotaxis using this new assay may have important diagnostic implications not only for burn patients but also for patients afflicted by other diseases that compromise neutrophil functions. 相似文献