全文获取类型
收费全文 | 22318篇 |
免费 | 2183篇 |
国内免费 | 2901篇 |
出版年
2024年 | 65篇 |
2023年 | 325篇 |
2022年 | 826篇 |
2021年 | 1327篇 |
2020年 | 998篇 |
2019年 | 1179篇 |
2018年 | 1037篇 |
2017年 | 802篇 |
2016年 | 1053篇 |
2015年 | 1545篇 |
2014年 | 1834篇 |
2013年 | 1886篇 |
2012年 | 2258篇 |
2011年 | 2214篇 |
2010年 | 1349篇 |
2009年 | 1152篇 |
2008年 | 1342篇 |
2007年 | 1117篇 |
2006年 | 951篇 |
2005年 | 784篇 |
2004年 | 626篇 |
2003年 | 584篇 |
2002年 | 472篇 |
2001年 | 153篇 |
2000年 | 154篇 |
1999年 | 152篇 |
1998年 | 144篇 |
1997年 | 101篇 |
1996年 | 94篇 |
1995年 | 73篇 |
1994年 | 86篇 |
1993年 | 49篇 |
1992年 | 68篇 |
1991年 | 64篇 |
1990年 | 50篇 |
1989年 | 45篇 |
1988年 | 32篇 |
1987年 | 25篇 |
1986年 | 24篇 |
1985年 | 49篇 |
1984年 | 23篇 |
1983年 | 20篇 |
1982年 | 28篇 |
1981年 | 25篇 |
1980年 | 22篇 |
1979年 | 15篇 |
1978年 | 19篇 |
1976年 | 20篇 |
1975年 | 15篇 |
1974年 | 19篇 |
排序方式: 共有10000条查询结果,搜索用时 31 毫秒
81.
几种昆虫生长调节剂对家白蚁的毒效试验 总被引:10,自引:2,他引:8
在室内条件下测定了卡死克、抑太保、灭幼豚3号、爱力螨克和扑虱灵五种昆虫生长调节剂对家白蚁的毒杀效果。初步筛选结果表明:卡死克、抑太保和爱力螨克对家白蚁的毒杀效果均较好,家白蚁对爱力螨克尤其敏感。2.30pm。yL爱力螨克、327.36pm0VL、卡死克和369.80V*wL抑太保处理白蚁5~6天后,其死亡率可达100%。忌避性试验表明:卡死克、抑太保和爱力螨克对家白蚁均无明显的驱避作用。 相似文献
82.
During periods of water deficit, plants accumulate late embryogenesis-abundant (LEA) proteins which are thought to protect cells from stresses associated with dehydration. One of these genes, le25, is expressed in tomato leaves and roots in response to water deficit and abscisic acid accumulation. To study the function of this protein and to test the effect of overproduction of the LE25 protein in Saccharomyces cerevisiae (Sc), a recombinant plasmid in which le25 is expressed under the control of the GAL1 promoter was constructed. The content of LE25 was high in Sc cells transformed with the recombinant plasmid. The transformant exhibited several stress-tolerant phenotypes. Growth of the transformant in a medium with 1.2 M NaCl was improved, as compared to a control strain. While the control strain showed a long lag phase of 40 h, le25-expressing cells showed a shortened lag phase of 10 h. However, no growth improvement was observed in a medium with 2 M sorbitol. In addition, the transformant had an increased survival rate after freezing stress, but not after high-temperature stress. These results, together with its predicted secondary structure, may indicate that LE25 functions as an ion scavenger. 相似文献
83.
84.
85.
We calculate the membrane-induced interaction between inclusions, in terms of the membrane stretching and bending moduli and the spontaneous curvature. We find that the membrane-induced interaction between inclusions varies nonmonotonically as a function of the inclusion spacing. The location of the energy minimum depends on the spontaneous curvature and the membrane perturbation decay length, where the latter is set by the membrane moduli. The membrane perturbation energy increases with the inclusion radius. The Ornstein-Zernike theory, with the Percus-Yevick closure, is used to calculate the radial distribution function of inclusions. We find that when the spontaneous curvature is zero, the interaction between inclusions due to the membrane deformation is qualitatively similar to the hard-core interaction. However, in the case of finite spontaneous curvature, the effective interaction is dramatically modified. 相似文献
86.
Noriko Usui Kouji Matsushima Anne M. Pilaro Dan L. Longo Robert H. Wiltrout 《Biotherapy》1996,9(4):199-208
Recombinant human interleukin 1α (rh IL-1α) and etoposide (VP-16) synergize for direct growth inhibition of several human
tumor cell linesin vitro. Our previous studies demonstrated that VP-16 increased the number of membrane-associated IL-1 receptors (IL-1Rs) and also
enhanced the internalization of receptor-bound rh IL-1α. The purposes of this study were to test our hypothess that these
events were critical to the synergy between rhIL-1α and VP-16, to determine whether rhIL-1α and VP-16 synergize to increase
superoxide (SO) anion radical productionin vitro since SO anion has been implicated in the toxic effects of IL-1, and to investigate the antitumor efficacy of the combinaton
against tumors in vivo. A375/C6 melanoma cells and OVCAR-3 ovarian carcinoma cells were tested with IL-1 receptor antagonist
(IL-1ra) before exposure to rhIL-1α, VP-16 and rhIL-1α plus VP-16. The synergistic or antagonistic effects were assessed by
MTT assay. SO production was measured by reduction of cytochrome C. Athymic female mice bearing the A375/C6 melanoma were
treated by rhIL-1α, VP-16, and rhIL-1α+VP-16. The antitumor effects were evaluated by quantitating tumor growth and survival
time. Pretreatment with the IL-1ra abrogated the synergistic effects of rhIL-1α and VP-16. The production of SO radical by
A375/C6 cells was increased 2.5 fold by the combination of rhIL-1α and VP-16, and the addition of exogenous SOD blocked the
synergy between rhIL-1α and VP-16. However, when A375/S0D15 cells which over-expressed manganese superoxide dismutase (MnSOD)
after MnSOD cDNA transfecton were exposed to rhIL-1α and VP-16, in vitro antagonism was observed. In vivo studies demonstrated
that the combination of rhIL-1α and VP-16 delayed tumor growth better than either agent alone, although long-term survival
was not improved because of substantial toxicity. Our results suggest that the synergistic antitumor effects of IL-1α and
VP-16 may be due to IL-1R modulation and increased internalization of IL-1-IL-1R complex by VP-16 treatment, as well as to
a subsequent increase in SO anion radical production from the tumor cells exposed to both drugs. Thus, the combnation of IL-1α
and VP-16 might prove useful for the treatment of malignant diseasein vivo, if the increased toxicity can be reduced or managed.
The US Government’s right to retain a non-exclusive royalty-free license on and to any copyright is acknowledged. 相似文献
87.
复合氨基酸在临床上的应用越来越被人们重视,我们利用水解猪血粉的方法得到含17种氨基酸的复合液,配制成滴眼剂用于多种原因引起的眼角膜溃疡疾病的治疗研究取得了较好的效果。 相似文献
88.
植食性同翅目昆虫在最终接受其宿主植物的过程中,将感受从叶表层直到韧皮部筛管途中存在的多种化学物质,并导致不同的刺探和取食行为。本文就植物叶表层存在的糖类和生物碱类物质,叶肉层的多糖类、酸碱度和酚类物质以及韧皮部内的氨基酸、蛋白质、碳水化合物和植物次生物质等对昆虫寻找及取食韧皮部行为的影响作一综述,并且还探讨了植物叶肉层的细胞结构对昆虫刺探及取食行为的可能影响。此外,结合刺探电位(electrical penetration graphs,EPGs)的研究结果,还讨论了昆虫感受植物叶组织内部的各种化学和细胞结构信息的可能机制。 相似文献
89.
90.
Paula A. Belinky Segula Masaphy Dan Levanon Yitzhak Hadar Carlos G. Dosoretz 《Applied microbiology and biotechnology》1994,40(5):629-633
The effect of nitrogen and fatty-acid-rich substrates on the production of 1-octen-3-ol by the edible fungus Pleurotus pulmonarius, during growth in both shaken flask and fermentor cultures, and in-vitro, in post-harvested mycelium, was studied. Addition of soybean flour and soybean oil to the growth medium enhanced 1-octen-3-ol production about sevenfold and doubled the fungal biomass, as compared to that obtained from P. pulmonarius cultured on a defined synthetic medium. A clear relationship between the production of 1-octen-3-ol and lipoxygenase activity was found during the growth of mushroom pellets. The highest in-vitro generation of 1-octen-3-ol was obtained upon addition of exogenous linoleic acid and pure O2 to pellets grown with soybean fluor and soybean oil. This generation was even higher than that of fruiting bodies exposed to the same conditions. These results suggest that lipoxygenase activity and, subsequently, 1-octen-3-ol biosynthesis in P. pulmonarius are enhanced by the presence of substrates containing fatty acids in the growth medium.
Correspondence to: D. Levanon 相似文献