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A synthesis of transplant experiments and ecological niche models suggests that range limits are often niche limits 下载免费PDF全文
Julie A. Lee‐Yaw Heather M. Kharouba Megan Bontrager Colin Mahony Anna Mária Csergő Annika M.E. Noreen Qin Li Richard Schuster Amy L. Angert 《Ecology letters》2016,19(6):710-722
Global change has made it important to understand the factors that shape species’ distributions. Central to this area of research is the question of whether species’ range limits primarily reflect the distribution of suitable habitat (i.e. niche limits) or arise as a result of dispersal limitation. Over‐the‐edge transplant experiments and ecological niche models are commonly used to address this question, yet few studies have taken advantage of a combined approach for inferring the causes of range limits. Here, we synthesise results from existing transplant experiments with new information on the predicted suitability of sites based on niche models. We found that individual performance and habitat suitability independently decline beyond range limits across multiple species. Furthermore, inferences from transplant experiments and niche models were generally concordant within species, with 31 out of 40 cases fully supporting the hypothesis that range limits are niche limits. These results suggest that range limits are often niche limits and that the factors constraining species’ ranges operate at scales detectable by both transplant experiments and niche models. In light of these findings, we outline an integrative framework for addressing the causes of range limits in individual species. 相似文献
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Daniel Eibach Ralf Krumkamp Hassan M. Al-Emran Nimako Sarpong Ralf Matthias Hagen Yaw Adu-Sarkodie Egbert Tannich Jürgen May 《PLoS neglected tropical diseases》2015,9(3)
BackgroundThe relevance of Cryptosporidium infections for the burden of childhood diarrhoea in endemic settings has been shown in recent years. This study describes Cryptosporidium subtypes among symptomatic and asymptomatic children in rural Ghana to analyse subtype-specific demographic, geographical, seasonal and clinical differences in order to inform appropriate control measures in endemic areas.Conclusions/SignificanceCryptosporidiosis is characterized by seasonal anthroponotic transmission of strains typically found in Sub-Saharan Africa. The infection mainly affects young infants, with vomiting and diarrhoea being one of the leading symptoms in C. parvum infection. Combining molecular typing and clinical data provides valuable information for physicians and is able to track sources of infections. 相似文献
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Jan Felix Drexler Victor Max Corman Florian Gloza-Rausch Antje Seebens Augustina Annan Anne Ipsen Thomas Kruppa Marcel A. Müller Elisabeth K. V. Kalko Yaw Adu-Sarkodie Samuel Oppong Christian Drosten 《PloS one》2009,4(7)
Background
Henipaviruses (Hendra and Nipah virus) are highly pathogenic members of the family Paramyxoviridae. Fruit-eating bats of the Pteropus genus have been suggested as their natural reservoir. Human Henipavirus infections have been reported in a region extending from Australia via Malaysia into Bangladesh, compatible with the geographic range of Pteropus. These bats do not occur in continental Africa, but a whole range of other fruit bats is encountered. One of the most abundant is Eidolon helvum, the African Straw-coloured fruit bat.Methodology/Principal Findings
Feces from E. helvum roosting in an urban setting in Kumasi/Ghana were tested for Henipavirus RNA. Sequences of three novel viruses in phylogenetic relationship to known Henipaviruses were detected. Virus RNA concentrations in feces were low.Conclusions/Significance
The finding of novel putative Henipaviruses outside Australia and Asia contributes a significant extension of the region of potential endemicity of one of the most pathogenic virus genera known in humans. 相似文献25.
Seyed Javad Davarpanah Seo Hee Jung Yaw Joo Kim Youn-Il Park Sung Ran Min Jang Ryol Liu Won Joong Jeong 《Journal of Plant Biology》2009,52(3):244-250
Plastids from Nicotiana benthamiana were transformed with the vector for dicistronic expression of two genes—aminoglycoside 3'-adenyltransferase (aadA) and green fluorescent protein (gfp)—in the plastids of Nicotiana tabacum. Transplastomic shoots exhibited green fluorescence under UV light. Transformation efficiencies were similar between species.
Although the border sequence (trnI and trnA) for homologous recombination to transform the plastid genome of N. benthamiana was identical to that sequence of N. tabacum, the exception was a 9-bp addition in the intron of trnI. This indicated that the N. tabacum sequence used as a border region for recombination was sufficient to insert the foreign gene into the target site between
the trnI and trnA of N. benthamiana with similar efficiency. Southern blot analysis detected the presence of aadA and gfp between trnI and trnA in the plastid genome of N. benthamiana. Northern and western blot analyses revealed high expression of gfp in the plastids from petals and leaves. Our results suggest that the plastid transformation system established here is applicable
to investigations of the interactions between plastid and nucleus in N. benthamiana. 相似文献
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Peter O. Aikpokpodion Juan C. Motamayor Victor O. Adetimirin Yaw Adu-Ampomah Ivan Ingelbrecht Albertus B. Eskes Raymond J. Schnell Maria Kolesnikova-Allen 《Tree Genetics & Genomes》2009,5(4):699-711
Knowledge of genebank and on-farm genetic diversity, particularly in an introduced crop species, is crucial to the management
and utilization of the genetic resources available. Microsatellite markers were used to determine genetic diversity in 574
accessions of cacao, Theobroma cacao L., representing eight groups covering parental populations in West Africa, genebank, and farmers’ populations in Nigeria.
From the 12 microsatellite markers used, a total of 144 alleles were detected with a mean allelic richness of 4.39 alleles/locus.
The largest genetic diversity was found in the Upper Amazon parent population (H
nb
= 0.730), followed by the 1944 Posnette’s Introduction (H
nb
= 0.704), and was lowest in the Local parent population (H
nb
= 0.471). Gene diversity was appreciably high in the farmers’ populations (H
nb
= 0.563–0.624); however, the effective number of alleles was lower than that found in the genebank’s Posnette’s population.
Fixation index estimates indicated deficiency of heterozygotes in the Upper Amazon and the Local parent populations (F
is
= 0.209 and 0.160, respectively), and excess of heterozygotes in the Trinitario parent population (F
is
= −0.341). The presence of inbreeding in the Local parent populations and substructure (Wahlund effect) in the Upper Amazon
were suggested for the deficiency of heterozygotes observed. Non-significant genetic differentiation observed between the
genebank’s and farmers’ populations indicated significant impact of national breeding programs on varieties grown in farmers’
plantations. From this study, we showed that appreciable genetic diversity was present in on-farm and field genebank collections
of cacao that can be exploited for crop improvement in West Africa. Suggestions for future conservation of on-farm genetic
diversity and local landraces are further discussed. 相似文献
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Homocysteine stimulates inducible nitric oxide synthase expression in macrophages: Antagonizing effect of ginkgolides and bilobalide 总被引:6,自引:0,他引:6
Hyperhomocysteinemia is an independent risk factor for atherosclerotic diseases. Inducible nitric oxide synthase (iNOS) is mainly expressed in macrophages upon stimulation. Overproduction of nitric oxide (NO) by iNOS can exacerbate the development of atherosclerosis. Our previous studies demonstrated that the extract of ginkgo biloba leaves (EGb) inhibited the iNOS-mediated NO production in monocyte-derived macrophage. We also reported that homocysteine could stimulate monocyte chemoattractant protein-1 (MCP-1) expression in vascular cells causing enhanced monocyte chemotaxis. The objective of the present study was to investigate the effect of homocysteine on iNOS-mediated NO production in macrophages and the antagonizing effect of EGb. Human monocytic cell (THP-1)-derived macrophages were incubated with homocysteine for various time periods. Homocysteine at concentrations of 0.05–0.1 mM significantly stimulated NO production and iNOS activity in macrophages via increased expression of iNOS mRNA and protein. The increased iNOS expression was associated with activation of nuclear factor-kappa B (NF-B) arising from reduced expression of inhibitor protein (IB) mRNA as well as increased phosphorylation of IB protein in homocysteine-treated cells. EGb and its terpenoids (ginkgolide A, ginkgolide B and bilobalide) could antagonize the homocysteine effect on iNOS expression in macrophages via their antioxidant effect resulting in attenuation of NF-B activation. Taken together, our results have demonstrated that homocysteine, at pathophysiological concentrations, stimulates iNOS-mediated NO production in macrophages. EGb and its terpenoids can antagonize such stimulatory effect via antioxidation and attenuation of NF-B activation. 相似文献
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Appiagyei-Dankah Y Tapiador CD Evans JF Castro-Magana M Aloia JF Yeh JK 《American journal of physiology. Endocrinology and metabolism》2003,284(3):E566-E573
The hypophysectomized rat has been used as a model to study the effects of growth hormone deficiency on bone. Here, we have investigated the influence of growth hormone administration to hypophysectomized rats (HX) for 6 wk on accumulation of triglycerides in bone marrow and on the differentiation of primary marrow stromal cells into adipocytes under in vitro conditions. We found that hypophysectomy significantly increased triglyceride concentration in bone marrow, which was attenuated by growth hormone administration. Primary bone marrow stromal cells derived from HX rats also had more adipocytes at confluence compared with growth hormone-treated hypophysectomized (GH) rats. When stimulated with 3-isobutyl-1-methylxanthine plus dexamethasone (IBMX-Dex), preadipocyte colony counts increased more significantly in GH rats. Markers of adipocyte differentiation were higher in HX than in control or GH rats at confluence. However, after stimulation with IBMX-Dex, increased expression of markers was seen in GH compared with HX rats. In conclusion, growth hormone administration to hypophysectomized rats attenuated triglyceride accumulation in bone marrow and inhibited the differentiation of stromal cells into adipocytes in vitro. 相似文献
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Interactions between proteins and heparin play a crucial role in most of the cellular process. Unraveling the forces that govern the formation of these complexes is vital for understanding the specificities involved in these biomolecular events. In the present study, a detailed analysis has been undertaken to evaluate the effect(s) of peptide conformation on heparin-binding, using a chimeric peptide, apaK6—a chimera of a highly stable neurotoxic peptide from honey-bee venom and a de novo designed lysine-rich peptide. The dissociation constants of these peptide–heparin complexes were found to be in the submicromolar range. Comparison of the results obtained from the titration of the disulfide-reduced and disulfide-intact chimeric peptide with various sulfated oligosaccharides, derived from heparin, suggest that the initial structure of the peptide has pronounced effect on the binding affinity, binding modes and also on binding preferences. The results of this study indicate that the heparin-binding specificity of an isolated peptide and that exhibited by the same peptide when present in a globular protein could be significantly different, especially if the isolated peptide undergoes conformational change(s) upon binding to the sulfated oligosaccharides. In addition, such dependency of the binding specificity on the preformed structures could be utilized for the design of high-affinity and sequence-specific heparin-binding polypeptides. 相似文献