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排序方式: 共有136条查询结果,搜索用时 15 毫秒
81.
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J Kawanami 《Journal of biochemistry》1967,62(1):105-117
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Thomas J. Carney Natália Martins Feitosa Carmen Sonntag Krasimir Slanchev Johannes Kluger Daiji Kiyozumi Jan M. Gebauer Jared Coffin Talbot Charles B. Kimmel Kiyotoshi Sekiguchi Raimund Wagener Heinz Schwarz Phillip W. Ingham Matthias Hammerschmidt 《PLoS genetics》2010,6(4)
Using forward genetics, we have identified the genes mutated in two classes of zebrafish fin mutants. The mutants of the first class are characterized by defects in embryonic fin morphogenesis, which are due to mutations in a Laminin subunit or an Integrin alpha receptor, respectively. The mutants of the second class display characteristic blistering underneath the basement membrane of the fin epidermis. Three of them are due to mutations in zebrafish orthologues of FRAS1, FREM1, or FREM2, large basement membrane protein encoding genes that are mutated in mouse bleb mutants and in human patients suffering from Fraser Syndrome, a rare congenital condition characterized by syndactyly and cryptophthalmos. Fin blistering in a fourth group of zebrafish mutants is caused by mutations in Hemicentin1 (Hmcn1), another large extracellular matrix protein the function of which in vertebrates was hitherto unknown. Our mutant and dose-dependent interaction data suggest a potential involvement of Hmcn1 in Fraser complex-dependent basement membrane anchorage. Furthermore, we present biochemical and genetic data suggesting a role for the proprotein convertase FurinA in zebrafish fin development and cell surface shedding of Fras1 and Frem2, thereby allowing proper localization of the proteins within the basement membrane of forming fins. Finally, we identify the extracellular matrix protein Fibrillin2 as an indispensable interaction partner of Hmcn1. Thus we have defined a series of zebrafish mutants modelling Fraser Syndrome and have identified several implicated novel genes that might help to further elucidate the mechanisms of basement membrane anchorage and of the disease''s aetiology. In addition, the novel genes might prove helpful to unravel the molecular nature of thus far unresolved cases of the human disease. 相似文献
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Akira Kimura Jun’ichi Kawanami Hideo Otsuka 《Bioscience, biotechnology, and biochemistry》2013,77(5):790-792
Calcineurin, which is a Ca2+/calmodulin-dependent protein phosphatase, is a key mediator in calcium signaling in diverse biological processes and of clinical importance as the target of the immunosuppressant FK506. To identify a mutant(s) in which calcineurin is activated, inhibiting cellular growth as a result, we screened for a mutant(s) whose temperature sensitivity would be suppressed by FK506 from the budding yeast non-essential gene deletion library. We found that the temperature sensitivity of cells in which the conserved Verprolin VRP1 gene had been deleted, which gene is required for actin organization and endocytosis, was suppressed by either FK506 or by cnb1 deletion. Indeed, the calcineurin activity increased significantly in the ?vrp1 cells. Finally, we demonstrated that the ?vrp1 strain to be useful as an indicator in a positive screening for bioactive compounds inhibiting calcineurin. 相似文献
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Naotoshi Sugimoto Osamu Shido Kentaro Matsuzaki Masanori Katakura Yoshiaki Hitomi Masao Tanaka Toshioki Sawaki Yoshimasa Fujita Takafumi Kawanami Yasufumi Masaki Toshiro Okazaki Hiroyuki Nakamura Shoichi Koizumi Akihiro Yachie Hisanori Umehara 《Cell biochemistry and biophysics》2014,70(1):301-307
Long-term continuous exposure to high ambient temperatures induces complete heat acclimation in humans and animals. However, to date, the effects of long-term exposure to heat stress on cells have not been fully evaluated. In this study, we investigated an adaptive physiological process induced in culture cells by continuous exposure to mild heat stress for 60 days. The results of this investigation provide evidence that after long-term heat acclimation in cells, (1) heat shock protein levels are increased, (2) hypoxia inducible factor-1α (HIF-1α) expression is upregulated, and (3) heat shock-induced and hypoxia-induced apoptoses are attenuated. These results suggest that the hypoxia response pathway is an intrinsic part of the heat acclimation repertoire and that the HIF-1 pathway following long-term heat acclimation induces cells with cross tolerance against hypoxia. 相似文献
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Shingo Noguchi Kazuhiro Yatera Ke-Yong Wang Keishi Oda Kentarou Akata Kei Yamasaki Toshinori Kawanami Hiroshi Ishimoto Yumiko Toyohira Hiroaki Shimokawa Nobuyuki Yanagihara Masato Tsutsui Hiroshi Mukae 《Respiratory research》2014,15(1):92