全文获取类型
收费全文 | 1249篇 |
免费 | 88篇 |
出版年
2022年 | 15篇 |
2021年 | 22篇 |
2020年 | 8篇 |
2019年 | 11篇 |
2018年 | 31篇 |
2017年 | 12篇 |
2016年 | 20篇 |
2015年 | 44篇 |
2014年 | 61篇 |
2013年 | 57篇 |
2012年 | 92篇 |
2011年 | 93篇 |
2010年 | 56篇 |
2009年 | 54篇 |
2008年 | 66篇 |
2007年 | 87篇 |
2006年 | 72篇 |
2005年 | 68篇 |
2004年 | 49篇 |
2003年 | 49篇 |
2002年 | 81篇 |
2001年 | 10篇 |
2000年 | 11篇 |
1999年 | 6篇 |
1998年 | 24篇 |
1997年 | 17篇 |
1996年 | 10篇 |
1995年 | 9篇 |
1994年 | 7篇 |
1993年 | 11篇 |
1992年 | 14篇 |
1991年 | 13篇 |
1990年 | 6篇 |
1989年 | 11篇 |
1988年 | 7篇 |
1987年 | 9篇 |
1986年 | 7篇 |
1985年 | 8篇 |
1984年 | 11篇 |
1982年 | 8篇 |
1981年 | 7篇 |
1980年 | 7篇 |
1979年 | 5篇 |
1977年 | 5篇 |
1976年 | 11篇 |
1974年 | 5篇 |
1973年 | 4篇 |
1972年 | 5篇 |
1971年 | 8篇 |
1969年 | 5篇 |
排序方式: 共有1337条查询结果,搜索用时 46 毫秒
191.
Scheel-Toellner D Raza K Assi L Pilling D Ross EJ Lee WY Curnow SJ Buckley CD Akbar AN Lord JM Salmon M 《Apoptosis : an international journal on programmed cell death》2008,13(1):109-117
Activated T cells require anti-apoptotic cytokines for their survival. The anti-apoptotic effects of these factors are mediated
by their influence on the balance of expression and localisation of pro- and anti-apoptotic members of the Bcl-2 family. Among
the anti-apoptotic Bcl-2 family members, the expression level of Bcl-2 itself and its interaction with the pro-apoptotic protein
Bim are now regarded as crucial for the regulation of survival in activated T cells. We studied the changes in Bcl-2 levels
and its subcellular distribution in relation to mitochondrial depolarisation and caspase activation in survival factor deprived
T cells. Intriguingly, the total Bcl-2 level appeared to remain stable, even after caspase 3 activation indicated entry into
the execution phase of apoptosis. However, cell fractionation experiments showed that while the dominant nuclear pool of Bcl-2
remained stable during apoptosis, the level of the smaller mitochondrial pool was rapidly downregulated. Signals induced by
anti-apoptotic cytokines continuously replenish the mitochondrial pool, but nuclear Bcl-2 is independent of such signals.
Mitochondrial Bcl-2 is lost rapidly by a caspase independent mechanism in the absence of survival factors, in contrast only
a small proportion of the nuclear pool of Bcl-2 is lost during the execution phase and this loss is a caspase dependent process.
We conclude that these two intracellular pools of Bcl-2 are regulated through different mechanisms and only the cytokine-mediated
regulation of the mitochondrial pool is relevant to the control of the initiation of apoptosis.
D. Scheel-Toellner and K. Raza have contributed equally to this study. 相似文献
192.
193.
A microdiversity study of anammox bacteria reveals a novel Candidatus Scalindua phylotype in marine oxygen minimum zones 总被引:4,自引:0,他引:4
Woebken D Lam P Kuypers MM Naqvi SW Kartal B Strous M Jetten MS Fuchs BM Amann R 《Environmental microbiology》2008,10(11):3106-3119
The anaerobic oxidation of ammonium (anammox) contributes significantly to the global loss of fixed nitrogen and is carried out by a deep branching monophyletic group of bacteria within the phylum Planctomycetes. Various studies have implicated anammox to be the most important process responsible for the nitrogen loss in the marine oxygen minimum zones (OMZs) with a low diversity of marine anammox bacteria. This comprehensive study investigated the anammox bacteria in the suboxic zone of the Black Sea and in three major OMZs (off Namibia, Peru and in the Arabian Sea). The diversity and population composition of anammox bacteria were investigated by both, the 16S rRNA gene sequences and the 16S-23S rRNA internal transcribed spacer (ITS). Our results showed that the anammox bacterial sequences of the investigated samples were all closely related to the Candidatus Scalindua genus. However, a greater microdiversity of marine anammox bacteria than previously assumed was observed. Both phylogenetic markers supported the classification of all sequences in two distinct anammox bacterial phylotypes: Candidatus Scalindua clades 1 and 2. Scalindua 1 could be further divided into four distinct clusters, all comprised of sequences from either the Namibian or the Peruvian OMZ. Scalindua 2 consisted of sequences from the Arabian Sea and the Peruvian OMZ and included one previously published 16S rRNA gene sequence from Lake Tanganyika and one from South China Sea sediment (97.9-99.4% sequence identity). This cluster showed only 相似文献
194.
195.
The viable propagule banks of a temporary stream were studied from sections with different agricultural history. Hatching
of zooplankton (copepods, rotifers and cladocerans) was recorded in the laboratory under controlled temperature and light
conditions from an agriculturally modified area with average hydroperiods of about a week per year and two semi-natural reference
areas with average hydroperiods of more than 3 weeks per year. We found significant differences in both taxon richness and
abundance of zooplankton hatching between areas, which were lower in the agriculturally modified section, compared to the
reference sections. Another factor likely to have influenced hatching in our experiment was conductivity, which differed between
the two reference sections and might have affected hatching at high conductivities. For restoration purposes, hydrological
reconnection of stream segments is important to facilitate dispersal from the high diversity upstream segments to the depleted
sites downstream. 相似文献
196.
Rosa Margesin Melanie Jud Dagmar Tscherko & Franz Schinner 《FEMS microbiology ecology》2009,67(2):208-218
Soil samples were collected along two slopes (south and north) at subalpine (1500–1900 m, under closed vegetation, up to the forest line) and alpine altitudes (2300–2530, under scattered vegetation, above the forest line) in the Grossglockner mountain area (Austrian central Alps). Soils were analyzed for a number of properties, including physical and chemical soil properties, microbial activity and microbial communities that were investigated using culture-dependent (viable heterotrophic bacteria) and culture-independent methods (phospholipid fatty acid analysis, FISH). Alpine soils were characterized by significantly ( P <0.01) colder climate conditions, i.e. lower mean annual air and soil temperatures, more frost and ice days and higher precipitation, compared with subalpine soils. Microbial activity (soil dehydrogenase activity) decreased with altitude; however, dehydrogenase activity was better adapted to cold in alpine soils compared with subalpine soils, as shown by the lower apparent optimum temperature for activity (30 vs. 37 °C) and the significantly ( P <0.01–0.001) higher relative activity in the low-temperature range. With increasing altitude, i.e. in alpine soils, a significant ( P <0.05–0.01) increase in the relative amount of culturable psychrophilic heterotrophic bacteria, in the relative amount of the fungal population and in the relative amount of Gram-negative bacteria was found, which indicates shifts in microbial community composition with altitude. 相似文献
197.
198.
199.
Siegfried Jahnke Marion I. Menzel Dagmar van Dusschoten Gerhard W. Roeb Jonas Bühler Senay Minwuyelet Peter Blümler Vicky M. Temperton Thomas Hombach Matthias Streun Simone Beer Maryam Khodaverdi Karl Ziemons Heinz H. Coenen Ulrich Schurr 《The Plant journal : for cell and molecular biology》2009,59(4):634-644
Unravelling the factors determining the allocation of carbon to various plant organs is one of the great challenges of modern plant biology. Studying allocation under close to natural conditions requires non-invasive methods, which are now becoming available for measuring plants on a par with those developed for humans. By combining magnetic resonance imaging (MRI) and positron emission tomography (PET), we investigated three contrasting root/shoot systems growing in sand or soil, with respect to their structures, transport routes and the translocation dynamics of recently fixed photoassimilates labelled with the short-lived radioactive carbon isotope 11 C. Storage organs of sugar beet ( Beta vulgaris ) and radish plants ( Raphanus sativus ) were assessed using MRI, providing images of the internal structures of the organs with high spatial resolution, and while species-specific transport sectoralities, properties of assimilate allocation and unloading characteristics were measured using PET. Growth and carbon allocation within complex root systems were monitored in maize plants ( Zea mays ), and the results may be used to identify factors affecting root growth in natural substrates or in competition with roots of other plants. MRI–PET co-registration opens the door for non-invasive analysis of plant structures and transport processes that may change in response to genomic, developmental or environmental challenges. It is our aim to make the methods applicable for quantitative analyses of plant traits in phenotyping as well as in understanding the dynamics of key processes that are essential to plant performance. 相似文献
200.