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131.
Zusammenfassung
Pinguicula lusitanica wurde in vitro auf stickstoff- und phorphorfreiem Mineralsalzagar kultiviert; jede Pflanze stand für sich in einem Erlenmeyerkolben.Als die Pflanzen 8 Wochen alt waren, wurden 20 Exemplare innerhalb von 5 Wochen viermal mit Pinuspollen gefüttet. 20 gleichgroße und gleichalte dienten als Kontrollen.Durch die Fütterung steigerte sich die Blattzahl und der Durchmesser der Blattrosette, die Blätter wurden intensiver grün und alterten langsamer.Vor allem wurde durch die Pollenfütterung die Blütenbildung ausgelöst. Schon nach der 2. Fütterung traten die ersten Knospen auf, eine Woche darauf blühten bereits 70% und noch vor der letzten Fütterung alle gefütterten Pflanzen, von den ungefütterten dagegen keine einzige. In dem anschließenden halben Jahr entwickelten die 20 gefütterten Pflanzen im ganzen 127 Blüten; die größte Blütenzahl einer Einzelpflanze was 14. Die nichtgefütterten Pflanzen waren auch jetzt noch rein vegetativ.
Mit Unterstützung durch die Deutsche Forschungsgemeinschaft 相似文献
Flowering of in vitro cultures of Pinguicula lusitanica after feeding with pinus pollen
Summary Plants of Pinguicula lusitanica were grown in individual Erlenmeyer flasks on an inorganic agar medium containing no nitrogen or phosphorus. After 8 weeks of culture, twenty of the plants were fed Pinus pollen 4 times over a period of 5 weeks.As a result of the feeding, the number of leaves as well as the diameters of the rosettes were increased. The leaves became turned a deeper green and aged more slowly.The most spectacular effect of the pollen feeding was an initiation of flowering. The first buds were already visible after the second feeding. All of the treated plants flowered before the last feeding, whereas none of the untreated plants flowered. During the following 6 months, the treated plants developed 127 flowers, the largest number on a single specimen being 14. Even after this period of time the untreated plants remained vegetative.
Mit Unterstützung durch die Deutsche Forschungsgemeinschaft 相似文献
132.
133.
134.
135.
Roger Griffiths Angus Grieve John Dunlop Inge Damgaard Hanne Fosmark Arne Schousboe 《Neurochemical research》1989,14(4):333-343
A detailed kinetic study of the inhibitory effects ofl- andd-enantiomers of cysteate, cysteine sulphinate, homocysteine sulphinate, homocysteate, and S-sulpho-cysteine on the neuronal, astroglial and synaptosomal high-affinity glutamate transport system was undertaken.d-[3H] Aspartate was used as the transport substrate. Kinetic characterisation of uptake in the absence of sulphur compounds confirmed the high-affinity nature of the transport systems, the Michaelis constant (K
m) ford-aspartate uptake being 6 M, 21 M and 84 M, respectively, in rat brain cortical synaptosomes and primary cultures of mouse cerebellar granule cells and cortical astrocytes. In those cases where significant effects could be demonstrated, the nature of the inhibition was competitive irrespective of the neuronal versus glial systems. The rank order of inhibition was essentially similar in synaptosomes, neurons and astrocytes. Potent inhibition (K
iK
m) of transport in each system was exhibited byl-cysteate, andl- andd-cysteine sulphinate whereas substantially weaker inhibitory effects (K
i>10–1000 times the appropriateK
m value) were exhibited by the remaining sulphur amino acids. In general, inhibition: (i) was markedly stereospecific in favor of thel-enantiomers (except for cysteine sulphinate) and (ii) was found to decrease with increasing chain length. Computer-assisted molecular modelling studies, in which volume contour maps of the sulphur compounds were superimposed on those ofd-aspartate andl-glutamate, demonstrated an order of inhibitory potency which was, qualitatively, in agreement with that obtained quantitatively by in vitro kinetic studies.Special issue dedicated to Dr. Elling Kvamme 相似文献
136.
Nils A. Øristland 《Bulletin of mathematical biology》1990,52(5):643-655
A computer model of body mass and composition in relation to gross energy balance is constructed. The model is built using
conventional empirical physiological formulae rather than statistical or analytical mathematical techniques. The model is
applied to the Minnesota and other experiments and produces as good or better simulations of observed values of changes in
body weight than reported for other formulae or models. Alternative physiological mechanisms concerning metabolic adaptions
to starvation, changes in time activity budgets and the energy equivalents of weight loss offer equally good simulations of
experimental results. The present analysis highlights the survival value of a basal metabolic depression during starvation
and indicates an optimal body composition of 10% mobilizable fat for starvation survival for a 70 kg man. Proper quantification
of the effects of the physiological mechanisms involved depends on new experimental data, however. Long term continuous monitoring
of time activity budgets are a necessary part of such experiments. 相似文献
137.
Summary
Candida tropicalis S001 was grown on the lipid fraction of a protein-containing waste-water in order to (i) remove fat from the water, and (ii) produre yeast biomass for feed. The yeast cells were separated from the waste-water by sedimentation. Defatted waste-water was used for methane production and gave a yield of a 0.3 m3 methane/kg reduced chemical oxygen demand. The maximum specific growth rate (µmax) of C. tropicalis growing on waste-water fat at pH 4.0 was 0.35 h–1; the fat content was decreased from 8 g/l to about 0.1 g/l within 24 h. In continous culture a corresponding reduction was maintained at dilution rates up to 0.36 h–1. The effect on growth of pH, temperature and CO2 concentration was studied with triolein as the major carbon source. The µmax was nearly constant (0.16 h–1) in the pH and temperature range of 3.2–4.0 and 30°–38° C, respectively; 10% CO2 was optimal for growth. Growth on triolein resulted in a biomass yield of 0.70 g dry weight/g fat.
Offprint requests to: S. Rydin 相似文献
138.
An De Bondt Kristel Eggermont Iris Penninckx Inge Goderis Willem F. Broekaert 《Plant cell reports》1996,15(7):549-554
We have previously developed a protocol for efficient gene transfer and regeneration of transgenic calli following cocultivation of apple (cv. Jonagold) explants with Agrobacterium tumefaciens (De Bondt et al. 1994, Plant Cell Reports 13: 587–593). Now we report on the optimization of postcultivation conditions for efficient and reproducible regeneration of transgenic shoots from the apple cultivar Jonagold. Factors which were found to be essential for efficient shoot regeneration were the use of gelrite as a gelling agent and the use of the cytokinin-mimicing thidiazuron in the selective postcultivation medium. Improved transformation efficiencies were obtained by combining the hormones thidiazuron and zeatin and by using leaf explants from in vitro grown shoots not older than 4 weeks after multiplication. Attempts to use phosphinothricin acetyl transferase as a selectable marker were not successful. Using selection on kanamycin under optimal postcultivation conditions, about 2% of the leaf explants developed transgenic shoots or shoot clusters. The presence and expression of the transferred genes was verified by -glucuronidase assays and Southern analysis. The transformation procedure has also been succesfully applied to several other apple cultivars.Abbreviations BAP
benzylaminopurine
- CTAB
hexadecyltrimethylammoniumbromide
- Na2EDTA
ethylenediamine-tetra-acetate ferric-sodium salt
- FeNaEDTA
ethylenediamine-tetra-acetate ferric-sodium salt
- GA3
gibberellic acid 3
- GusA
-glucuronidase
-
gusA
-glucuronidase gene of Escherichia coli
- IAA
indole acetic acid
- IBA
indole butyric acid
- 2iP
N6-2-isopentenyl adenine
- NAA
naphthalene acetic acid
-
nptII
neomycinphosphotransferase II gene
-
bar
phosphinothricin acetyl transferase gene
- PCR
polymerase chain reaction
- PPT
phosphinothricin
- STS
silver thiosulphate
- T-DNA
transferred DNA
- TDZ
thidiazuron
- X-Gluc
5-bromo-4-chloro-3-indolyl -D-glucuronide
- Zea
trans-Zeatin 相似文献
139.
Independently of their agonistic or antagonistic activity on different isolated tissue preparations, the kinin analogues investigated induce histamine release on rat peritoneal mast cells. The effectivity of most compounds is 10 to 100 times higher than that of bradykinin. Beside the positively charged amino acids, the elongation at the N-terminus with hydrophobic amino acids and the replacement of amino acids in the bradykinin sequence (especially at position 7) with aromatic residues is important for a high histamine-releasing activity. 相似文献
140.
Kristel Eggermont Inge J. Goderis Willem F. Broekaert 《Plant Molecular Biology Reporter》1996,14(3):273-279
We describe here a reliable high-throughput method for extraction of RNA from fresh or frozen plant tissue that obviates laborious
and time-consuming homogenisation by mortar and pestle. The method is based on homogenisation by high-speed reciprocal shaking
in presence of a mixture of inexpensive abrasive materials; i.e., quartz sand and glass beads. After homogenisation, the method
follows a standard procedure for RNA extraction by phenol/LiCl. Yield and quality of RNA obtained by homogenisation with the
sand/glass bead mix are identical to those obtained by mortar and pestle. 相似文献