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961.
So Yung Yang Ji Hyun Baek Youngah Cho Eun-Young Cho Yujin Choi Yongkang Kim 《Chronobiology international》2018,35(3):405-415
ST8SIA2 and NCAM1 are functionally related genes forming polysialic acid (PSA) - neural cell adhesion molecule (NCAM) complex in suprachiasmatic nucleus (SCN), the regulating site of circadian biological rhythm. In this study, the relationship of ST8SIA2 and NCAM1 with circadian and seasonal rhythms of human behavior was explored. Subjects were 261 healthy Korean adults who were free of any history of clinically significant psychiatric symptoms. The phenotypes were circadian preference and seasonal change of mood and behavior (seasonality) measured by the Composite Scale of Morningness and the Seasonal Pattern Assessment Questionnaire, respectively. Thirty-four single nucleotide polymorphisms (SNPs) across the ST8SIA2 region and 15 SNPs of NCAM1 were analyzed. A nominally significant association with seasonality and circadian preference was observed in 21 variants of both genes. After corrections for multiple testing, associations of 8 SNPs of ST8SIA2 and 2 SNPs of NCAM1 with seasonality remained significant. Some of these SNPs were also associated with psychiatric disorders in previous studies. This study demonstrated a meaningful and/or suggestive evidence of association between behavioral phenotypes reflecting human biological rhythm and two interplaying genes involved in the plasticity of SCN’s neuronal network. 相似文献
962.
963.
964.
Jung Won Shin Sung Hee Choi Dong Eun Kim Hee Sook Kim Jae-Hwa Lee In Su Lee Eun Yeol Lee 《Bioprocess and biosystems engineering》2011,34(1):113-119
The marine alginate lyase from Streptomyces sp. ALG-5, which specifically degrades poly-G block of alginate, was functionally expressed as a His-tagged form with an
Escherichia coli expression system. The recombinant alginate lyase expressed with pColdI at 15 °C exhibited the highest alginate-degrading
activity. The recombinant alginate lyase was efficiently immobilized onto two types of magnetic nanoparticles, superparamagnetic
iron oxide nanoparticle, and hybrid magnetic silica nanoparticle, based on the affinity between His-tag and Ni2+ that displayed on the surfaces of nanoparticles. An alginate oligosaccharide mixture consisting of dimer and trimer was prepared
by the immobilized alginate lyase. The immobilized enzymes were re-used repeatedly more than 10 times after magnetic separation. 相似文献
965.
966.
The objectives of this study were to test the effects of light on melatonin rhythms in the pineal gland and gut of goldfish Carassius auratus and to investigate whether melatonin function differed in these two tissues, which are photosensitive and non-photosensitive respectively. Rhythms were evaluated by measuring arylalkylamine N-acetyltransferase (AANAT2) and melatonin receptor 1 (MT-R1) mRNA expression and melatonin concentration in the pineal gland, gut (in vivo), and cell cultures of the two tissues (in vitro). Compared to control, pineal gland melatonin secretion was higher at night, whereas the 24-h dark and ophthalmectomy groups maintained higher AANAT2 and MT-R1 mRNA expression during the day. Melatonin levels and AANAT2 and MT-R1 mRNA expression in the gut were also the highest at night, but the 24-h light, dark, and ophthalmectomy groups did not significantly differ from control. Furthermore, we measured AANAT2 and MT-R1 mRNA expression in high temperature water (30 °C) to investigate differences in the antioxidant capacity of pineal gland vs. gut melatonin. Melatonin and H2O2 levels, as well as AANAT2 and MT-R1 mRNA expression, were all higher in the two tissues under thermal stress, compared with their levels at 22 °C. Taken together, our results suggest that light has no effect on melatonin patterns in the gut, which appears to exhibit its own circadian rhythm, but both gut and pineal gland melatonin exhibit similar antioxidant function. 相似文献
967.
Yi SS Hwang IK Kim DW Shin JH Nam SM Choi JH Lee CH Won MH Seong JK Yoon YS 《Neurochemical research》2011,36(1):117-128
Because it appears that oxidative stress and inflammation are implicated with disease pathogenesis in the diabetic brain,
many researchers have used streptozotocin (STZ)-induced diabetic animals to study superoxide production and the effects of
superoxide scavengers like Cu,Zn-superoxide dismutase (SOD1). However, many studies have been conducted without considering
temporal changes after STZ injection. Interestingly, though SOD activities were not significantly different among the groups,
SOD1 and 4-hydroxy-2-nonenal (4-HNE) immunoreactivities were significantly enhanced at 3 weeks after an STZ injection (STZ3w)
versus only marginal levels in sham controls, whereas microglial activity was remarkably reduced in injected rats at this
time. However, SOD1 immunoreactivity and microglial activities were only at the sham level at STZ4w. The present study provides
important information concerning cell damage by ROS generated by STZ. Microglial response was found to be inactivated at STZ3w
and neuronal cells (NeuN) showed a non-significant tendency to be reduced in number at STZ4w except in the dentate gyrus.
We speculated that the above oxidative stress-related events should be accomplished at STZ3w in the brains of STZ-induced
diabetes animal models. Therefore, the aim of the present study was to investigate chronological changes in SOD1 immunoreactivity
associated with lipid peroxidation and inflammatory responses in the hippocampi of STZ-induced type I diabetic rats. 相似文献
968.
969.
Ji JE Kim SK Ahn KH Choi JM Jung SY Jung KM Jeon HJ Kim DK 《Prostaglandins & other lipid mediators》2011,94(3-4):88-95
Ceramide has been suggested to function as a mediator of exocytosis in response to the addition of a calcium ionophore from PC12 cells. Here, we show that although cell-permeable C(6)-ceramide or a calcium ionophore alone did not increase either the degranulation of serotonin or the release of arachidonic acid (AA) from RBL-2H3 cells, their combined effect significantly stimulated these processes in a time- and dose-dependent manner. This effect was inhibited by the presence of an exogenous calcium chelator and significantly suppressed by the CERK inhibitor (K1) and phospholipase A(2) (PLA(2)) inhibitors. Moreover, cytosolic PLA(2) GIVA (cPLA(2) GIVA) siRNA-transfected RBL-2H3 cells showed a lower level of serotonin release than scramble siRNA-transfected cells. Little is known about the regulation of degranulation proximal to the activation of cytosolic phospholipase A(2) GIVA, the initial rate-limiting step in RBL-2H3 cells. In this study, we suggest that CERK, ceramide-1-phosphate, and PLA(2) are involved in degranulation in a calcium-dependent manner. Inhibition of p44/p42 mitogen-activated protein kinase partially decreased the AA release, but did not affect degranulation. Furthermore, treatment of the cells with AA (ω-6, C20:4), not linoleic acid (ω-6, C18:2) or α-linolenic acid (ω-6, C18:3), induced degranulation. Taken together, these results suggest that ceramide is involved in mast cell degranulation via the calcium-mediated activation of PLA(2). 相似文献
970.
Hsing CH Lin MC Choi PC Huang WC Kai JI Tsai CC Cheng YL Hsieh CY Wang CY Chang YP Chen YH Chen CL Lin CF 《PloS one》2011,6(3):e17598