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61.
We describe here a new method for specific staining of mast cells using ferroin. Different hamster tissues were fixed in 4% formalin and processed for paraffin embedding. Sections were stained with hematoxylin followed by ferroin acidified with 2.5 N sulfuric acid to pH 4.0. Mast cells stained an intense orange color that contrasted markedly with bluish violet nuclei. High contrast was also observed when ferroin colored sections were counterstained with light green instead of hematoxylin. To evaluate the specificity of the stain, hamster cheek pouch sections were stained with toluidine blue, alcian blue-safranin O, and ferroin. Quantitative evaluation of mast cells stained with the three techniques showed no statistical difference. The simplicity and selectivity of this method is sufficient for image analysis of mast cells. 相似文献
62.
C. J. Fettig S. R. McKelvey C. P. Dabney R. R. Borys D. P. W. Huber 《Journal of Applied Entomology》2009,133(2):143-154
A blend of eight nonhost angiosperm volatiles (benzyl alcohol, benzaldehyde, guaiacol, nonanal, salicylaldehyde, (E)‐2‐hexenal, (E)‐2‐hexen‐1‐ol and (Z)‐2‐hexen‐1‐ol) without [NAV] and with [NAVV] (–)‐verbenone (4,6,6‐trimethylbicyclo[3.1.1]hept‐3‐en‐2‐one) were tested at low (L), medium (M) and high (H) release rates for their ability to reduce attraction of western pine beetle, Dendroctonus brevicomis LeConte, to attractant‐baited (exo‐brevicomin [racemic, 3 mg/d], frontalin [racemic, 3 mg/d] and myrcene [18 mg/d]) multiple funnel traps. NAV‐L (40 mg/d) had no significant effect. Verbenone alone (50 mg/d) and NAV‐M (240 mg/d) both significantly reduced attraction, but no significant difference was observed between the two treatment means. NAV‐H (430 mg/d) significantly reduced catches by ~60% and 78% compared to verbenone alone and the baited control, respectively. In a second experiment, combining (–)‐verbenone with NAV (NAVV) increased the effects observed in Experiment 1. NAVV‐M (240 mg/d) resulted in an ~69% and 83% reduction in trap catch compared to verbenone alone and the baited control, respectively. Significantly fewer D. brevicomis were captured in NAVV‐H (430 mg/d) than any other treatment resulting in an ~93% reduction in trap catch compared to the baited control. In a third experiment, NAVV was tested at three release rates for its ability to protect individual ponderosa pines, Pinus ponderosa Dougl. ex Laws., from attack by D. brevicomis. Cumulative release rates varied in direct proportion to tree diameter, but represented quarter, half and full NAVV rates. NAVV significantly reduced the density of D. brevicomis attacks, D. brevicomis successful attacks, and levels of tree mortality on attractant‐baited trees. Only three of 15 NAVV‐treated trees died from D. brevicomis attack while ~93% mortality (14/15) was observed in the untreated, baited control. Quarter and half rates were ineffective for reducing tree mortality. 相似文献
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The DNA binding characteristics of a series of homologous 2,6-disubstituted anthraquinone threading intercalators bearing one to four ethylene glycol units in their side chains have been studied. Binding constants were measured via surface plasmon resonance (SPR). These compounds bind to an AT-rich hairpin with slightly higher affinity than to a GC-rich hairpin. The binding constants decrease as the length of the side chain increases. 相似文献
65.
Sophisticated molecular biological research has revealed many virulence attributes in at least four pathogenic fungi, but the future study of fungal virulence requires investigators to distinguish between molecules that directly interact with the host, molecules that regulate these, and molecules that are always required for fungal growth and survival, independent of the host. 相似文献
66.
T Williams E Walz AR Lane M Pebole AC Hackney 《Biology of sport / Institute of Sport》2015,32(3):193-198
This study assessed the influence of estrogen (E2) on muscle damage biomarkers [skeletal muscle - creatine kinase (CK); cardiac muscle - CK-MB] responses to prolonged aerobic exercise. Eumenorrheic women (n=10) who were physically active completed two 60-minute treadmill running sessions at ∼60-65% maximal intensity during low E2 (midfollicular menstrual phase) and high E2 (midluteal menstrual phase) hormonal conditions. Blood samples were collected prior to exercise (following supine rest), immediately post-, 30 min post-, and 24 hours post-exercise to determine changes in muscle biomarkers. Resting blood samples confirmed appropriate E2 hormonal levels Total CK concentrations increased following exercise and at 24 hours post-exercise were higher in the midfollicular low E2 phase (p<0.001). However, CK-MB concentrations were unaffected by E2 level or exercise (p=0.442) resulting in the ratio of CK-MB to total CK being consistently low in subject responses (i.e., indicative of skeletal muscle damage). Elevated E2 levels reduce the CK responses of skeletal muscle, but had no effect on CK-MB responses following prolonged aerobic exercise. These findings support earlier work showing elevated E2 is protective of skeletal muscle from exercise-induced damage associated with prolonged aerobic exercise. 相似文献
67.
In normalizing two-channel expression arrays, the ANOVA approach explicitly incorporates the experimental design in its model, and the MA plot-based approach accounts for intensity-dependent biases. However, both approaches can lead to inaccurate normalization in fairly common scenarios. We propose a method called efficient Common Array Dye Swap (eCADS) for normalizing two-channel microarrays that accounts for both experimental design and intensity-dependent biases. Under reasonable experimental designs, eCADS preserves differential expression relationships and requires only a single array per sample pair. 相似文献
68.
A two-channel microarray measures the relative expression levels of thousands of genes from a pair of biological samples. In order to reliably compare gene expression levels between and within arrays, it is necessary to remove systematic errors that distort the biological signal of interest. The standard for accomplishing this is smoothing "MA-plots" to remove intensity-dependent dye bias and array-specific effects. However, MA methods require strong assumptions, which limit their general applicability. We review these assumptions and derive several practical scenarios in which they fail. The "dye-swap" normalization method has been much less frequently used because it requires two arrays per pair of samples. We show that a dye-swap is accurate under general assumptions, even under intensity-dependent dye bias, and that a dye-swap removes dye bias from a single pair of samples in general. Based on a flexible model of the relationship between mRNA amount and single-channel fluorescence intensity, we demonstrate the general applicability of a dye-swap approach. We then propose a common array dye-swap (CADS) method for the normalization of two-channel microarrays. We show that CADS removes both dye bias and array-specific effects, and preserves the true differential expression signal for every gene under the assumptions of the model. 相似文献
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